Literature DB >> 1702378

In situ nick translation of human metaphase chromosomes with the restriction enzymes MspI and HpaII reveals an R-band pattern.

S Adolph1, H Hameister.   

Abstract

Several restriction enzymes (HindIII, HaeIII, MspI, HpaII, EcoRI, KpnI, and NotI) were evaluated for their ability to induce bands in human metaphase chromosomes during in situ nick translation. MspI and HpaII were able to induce a completely developed R-band pattern. Preferential cleavage of R-band chromatin is due to the presence of unmethylated CpG-residues present in CpG-rich islands, which are apparently unevenly distributed and mainly concentrated in R-bands.

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Year:  1990        PMID： 1702378     DOI： 10.1159/000132976

Source DB:  PubMed          Journal:  Cytogenet Cell Genet        ISSN： 0301-0171


  8 in total

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2.  Restriction endonuclease/nick translation procedure on fixed chromosomes of the Atlantic salmon fish cell line.

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3.  Non-uniform distribution of methylatable CCGG sequences on human chromosomes as shown by in situ methylation.

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4.  New sites of methylcytosine-rich DNA detected on metaphase chromosomes.

Authors:  A Barbin; C Montpellier; N Kokalj-Vokac; A Gibaud; A Niveleau; B Malfoy; B Dutrillaux; C A Bourgeois
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5.  DNA methylation of the X chromosomes of the human female: an in situ semi-quantitative analysis.

Authors:  J Bernardino; E Lamoliatte; M Lombard; A Niveleau; B Malfoy; B Dutrillaux; C A Bourgeois
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6.  In human chromosomes telomeric regions are enriched in CpGs relative to R-bands.

Authors:  M Ferraro; V Predazzi; G Prantera
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Review 7.  The distribution of genes on chromosomes: a cytological approach.

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8.  Common methylation characteristics of sex chromosomes in somatic and germ cells from mouse, lemur and human.

Authors:  J Bernardino; M Lombard; A Niveleau; B Dutrillaux
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  8 in total

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