Literature DB >> 8419306

Membrane topology model of Escherichia coli alpha-ketoglutarate permease by phoA fusion analysis.

W Seol1, A J Shatkin.   

Abstract

Escherichia coli alpha-ketoglutarate permease (KgtP) is a 432-amino-acid protein that symports alpha-ketoglutarate and protons. KgtP was predicted to contain 12 membrane-spanning domains on the basis of a calculated hydropathy profile. The membrane topology model of KgtP was analyzed by using kgtP-phoA gene fusions and measuring alkaline phosphatase activities in cells expressing the chimeric proteins. Comparisons of the phosphatase activity levels and the locations of the KgtP-PhoA junctions are consistent with the predicted membrane topology model of KgtP.

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Year:  1993        PMID： 8419306      PMCID： PMC196175          DOI： 10.1128/jb.175.2.565-567.1993

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  16 in total

1.  The use of transposon TnphoA to detect genes for cell envelope proteins subject to a common regulatory stimulus. Analysis of osmotically regulated genes in Escherichia coli.

Authors:  C Gutierrez; J Barondess; C Manoil; J Beckwith
Journal:  J Mol Biol       Date:  1987-05-20       Impact factor: 5.469

2.  Fusions of secreted proteins to alkaline phosphatase: an approach for studying protein secretion.

Authors:  C S Hoffman; A Wright
Journal:  Proc Natl Acad Sci U S A       Date:  1985-08       Impact factor: 11.205

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Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  A comprehensive set of sequence analysis programs for the VAX.

Authors:  J Devereux; P Haeberli; O Smithies
Journal:  Nucleic Acids Res       Date:  1984-01-11       Impact factor: 16.971

5.  Escherichia coli alpha-ketoglutarate permease is a constitutively expressed proton symporter.

Authors:  W Seol; A J Shatkin
Journal:  J Biol Chem       Date:  1992-03-25       Impact factor: 5.157

6.  Site-directed mutants of Escherichia coli alpha-ketoglutarate permease (KgtP).

Authors:  W Seol; A J Shatkin
Journal:  Biochemistry       Date:  1992-04-07       Impact factor: 3.162

7.  Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes.

Authors:  F W Studier; B A Moffatt
Journal:  J Mol Biol       Date:  1986-05-05       Impact factor: 5.469

8.  Membrane topology analysis of Escherichia coli mannitol permease by using a nested-deletion method to create mtlA-phoA fusions.

Authors:  J E Sugiyama; S Mahmoodian; G R Jacobson
Journal:  Proc Natl Acad Sci U S A       Date:  1991-11-01       Impact factor: 11.205

9.  TnphoA: a transposon probe for protein export signals.

Authors:  C Manoil; J Beckwith
Journal:  Proc Natl Acad Sci U S A       Date:  1985-12       Impact factor: 11.205

Review 10.  Homologous sugar transport proteins in Escherichia coli and their relatives in both prokaryotes and eukaryotes.

Authors:  P J Henderson; M C Maiden
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  1990-01-30       Impact factor: 6.237
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  11 in total

1.  The N-terminal region of the Escherichia coli WecA (Rfe) protein, containing three predicted transmembrane helices, is required for function but not for membrane insertion.

Authors:  A O Amer; M A Valvano
Journal:  J Bacteriol       Date:  2000-01       Impact factor: 3.490

Review 2.  Osmosensing by bacteria: signals and membrane-based sensors.

Authors:  J M Wood
Journal:  Microbiol Mol Biol Rev       Date:  1999-03       Impact factor: 11.056

Review 3.  Linkage map of Escherichia coli K-12, edition 10: the traditional map.

Authors:  M K Berlyn
Journal:  Microbiol Mol Biol Rev       Date:  1998-09       Impact factor: 11.056

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Authors:  I T Paulsen; M H Brown; R A Skurray
Journal:  Microbiol Rev       Date:  1996-12

5.  Multidrug resistance proteins QacA and QacB from Staphylococcus aureus: membrane topology and identification of residues involved in substrate specificity.

Authors:  I T Paulsen; M H Brown; T G Littlejohn; B A Mitchell; R A Skurray
Journal:  Proc Natl Acad Sci U S A       Date:  1996-04-16       Impact factor: 11.205

Review 6.  Computer-aided analyses of transport protein sequences: gleaning evidence concerning function, structure, biogenesis, and evolution.

Authors:  M H Saier
Journal:  Microbiol Rev       Date:  1994-03

7.  Ketoglutarate transport protein KgtP is secreted through the type III secretion system and contributes to virulence in Xanthomonas oryzae pv. oryzae.

Authors:  Wei Guo; Lu-Lu Cai; Hua-Song Zou; Wen-Xiu Ma; Xi-Ling Liu; Li-Fang Zou; Yu-Rong Li; Xiao-Bin Chen; Gong-You Chen
Journal:  Appl Environ Microbiol       Date:  2012-06-08       Impact factor: 4.792

Review 8.  Major facilitator superfamily.

Authors:  S S Pao; I T Paulsen; M H Saier
Journal:  Microbiol Mol Biol Rev       Date:  1998-03       Impact factor: 11.056

9.  Transcriptional Control of Dual Transporters Involved in α-Ketoglutarate Utilization Reveals Their Distinct Roles in Uropathogenic Escherichia coli.

Authors:  Wentong Cai; Xuwang Cai; Yongwu Yang; Shigan Yan; Haibin Zhang
Journal:  Front Microbiol       Date:  2017-02-21       Impact factor: 5.640

10.  Topological analysis of a haloacid permease of a Burkholderia sp. bacterium with a PhoA-LacZ reporter.

Authors:  Yuk Man Tse; Manda Yu; Jimmy S H Tsang
Journal:  BMC Microbiol       Date:  2009-10-31       Impact factor: 3.605
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