Literature DB >> 10629198

The N-terminal region of the Escherichia coli WecA (Rfe) protein, containing three predicted transmembrane helices, is required for function but not for membrane insertion.

A O Amer1, M A Valvano.   

Abstract

The correct site for translation initiation for Escherichia coli WecA (Rfe), presumably involved in catalyzing the transfer of N-acetylglucosamine 1-phosphate to undecaprenylphosphate, was determined by using its FLAG-tagged derivatives. The N-terminal region containing three predicted transmembrane helices was found to be necessary for function but not for membrane localization of this protein.

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Year:  2000        PMID： 10629198      PMCID： PMC94301          DOI： 10.1128/JB.182.2.498-503.2000

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  36 in total

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Journal:  Glycobiology       Date:  1998-06       Impact factor: 4.313

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Authors:  M J Osborn; J E Gander; E Parisi; J Carson
Journal:  J Biol Chem       Date:  1972-06-25       Impact factor: 5.157

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Authors:  U Meier-Dieter; R Starman; K Barr; H Mayer; P D Rick
Journal:  J Biol Chem       Date:  1990-08-15       Impact factor: 5.157

Review 5.  Bacterial polysaccharide synthesis and gene nomenclature.

Authors:  P R Reeves; M Hobbs; M A Valvano; M Skurnik; C Whitfield; D Coplin; N Kido; J Klena; D Maskell; C R Raetz; P D Rick
Journal:  Trends Microbiol       Date:  1996-12       Impact factor: 17.079

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Authors:  J D Klena; C A Schnaitman
Journal:  Mol Microbiol       Date:  1993-07       Impact factor: 3.501

7.  Nucleotide sequence of the Escherichia coli rfe gene involved in the synthesis of enterobacterial common antigen. Molecular cloning of the rfe-rff gene cluster.

Authors:  U Meier-Dieter; K Barr; R Starman; L Hatch; P D Rick
Journal:  J Biol Chem       Date:  1992-01-15       Impact factor: 5.157

8.  Genetic analysis of the O-specific lipopolysaccharide biosynthesis region (rfb) of Escherichia coli K-12 W3110: identification of genes that confer group 6 specificity to Shigella flexneri serotypes Y and 4a.

Authors:  Z Yao; M A Valvano
Journal:  J Bacteriol       Date:  1994-07       Impact factor: 3.490

9.  Role of the rfe gene in the synthesis of the O8 antigen in Escherichia coli K-12.

Authors:  P D Rick; G L Hubbard; K Barr
Journal:  J Bacteriol       Date:  1994-05       Impact factor: 3.490

10.  Role of the rfe gene in the biosynthesis of the Escherichia coli O7-specific lipopolysaccharide and other O-specific polysaccharides containing N-acetylglucosamine.

Authors:  D C Alexander; M A Valvano
Journal:  J Bacteriol       Date:  1994-11       Impact factor: 3.490
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  13 in total

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2.  Membrane topology and identification of critical amino acid residues in the Wzx O-antigen translocase from Escherichia coli O157:H4.

Authors:  Cristina L Marolda; Bo Li; Michael Lung; Mei Yang; Anna Hanuszkiewicz; Amanda Roa Rosales; Miguel A Valvano
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3.  Analysis of the Topology and Active-Site Residues of WbbF, a Putative O-Polysaccharide Synthase from Salmonella enterica Serovar Borreze.

Authors:  Samantha S Wear; Brittany A Hunt; Bradley R Clarke; Chris Whitfield
Journal:  J Bacteriol       Date:  2020-02-11       Impact factor: 3.490

4.  Defective O-antigen polymerization in tolA and pal mutants of Escherichia coli in response to extracytoplasmic stress.

Authors:  Enrique D Vinés; Cristina L Marolda; Aran Balachandran; Miguel A Valvano
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5.  Fluorescence-based assay for polyprenyl phosphate-GlcNAc-1-phosphate transferase (WecA) and identification of novel antimycobacterial WecA inhibitors.

Authors:  Katsuhiko Mitachi; Shajila Siricilla; Dong Yang; Ying Kong; Karolina Skorupinska-Tudek; Ewa Swiezewska; Scott G Franzblau; Michio Kurosu
Journal:  Anal Biochem       Date:  2016-08-13       Impact factor: 3.365

6.  Functional characterization and membrane topology of Escherichia coli WecA, a sugar-phosphate transferase initiating the biosynthesis of enterobacterial common antigen and O-antigen lipopolysaccharide.

Authors:  Jason Lehrer; Karen A Vigeant; Laura D Tatar; Miguel A Valvano
Journal:  J Bacteriol       Date:  2007-01-19       Impact factor: 3.490

7.  A novel outer membrane protein, Wzi, is involved in surface assembly of the Escherichia coli K30 group 1 capsule.

Authors:  Andrea Rahn; Kostantinos Beis; James H Naismith; Chris Whitfield
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8.  Chemoenzymatic synthesis of the bacterial polysaccharide repeating unit undecaprenyl pyrophosphate and its analogs.

Authors:  Lei Li; Robert L Woodward; Weiqing Han; Jingyao Qu; Jing Song; Cheng Ma; Peng G Wang
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9.  Purification and characterization of the bacterial UDP-GlcNAc:undecaprenyl-phosphate GlcNAc-1-phosphate transferase WecA.

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10.  DNA polymorphism analysis of Brucella lipopolysaccharide genes reveals marked differences in O-polysaccharide biosynthetic genes between smooth and rough Brucella species and novel species-specific markers.

Authors:  Michel S Zygmunt; José M Blasco; Jean-Jacques Letesson; Axel Cloeckaert; Ignacio Moriyón
Journal:  BMC Microbiol       Date:  2009-05-13       Impact factor: 3.605
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