Literature DB >> 2999794

TnphoA: a transposon probe for protein export signals.

C Manoil, J Beckwith.   

Abstract

We constructed a derivative of transposon Tn5 that permits the generation of hybrid proteins composed of alkaline phosphatase (EC 3.1.3.1) lacking its signal peptide fused to amino-terminal sequences of other proteins. Such a hybrid gives alkaline phosphatase activity if the protein fused to alkaline phosphatase contributes sequences that promote export and thus compensate for the missing alkaline phosphatase signal peptide. Fusions to both a secreted periplasmic protein and a complex cytoplasmic membrane protein led to alkaline phosphatase activity. TnphoA fusions should help localize export signals within the structure of a protein, such as a transmembrane protein, as well as identify new chromosomal genes for secreted and transmembrane proteins.

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Year:  1985        PMID： 2999794      PMCID： PMC391456          DOI： 10.1073/pnas.82.23.8129

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  28 in total

1.  Mating aggregates in Escherichia coli conjugation.

Authors:  M Achtman
Journal:  J Bacteriol       Date:  1975-08       Impact factor: 3.490

2.  Use of gene fusion to study secretion of maltose-binding protein into Escherichia coli periplasm.

Authors:  P J Bassford; T J Silhavy; J R Beckwith
Journal:  J Bacteriol       Date:  1979-07       Impact factor: 3.490

3.  Complete nucleotide sequence of the Escherichia coli plasmid pBR322.

Authors:  J G Sutcliffe
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1979

4.  Fusions of secreted proteins to alkaline phosphatase: an approach for studying protein secretion.

Authors:  C S Hoffman; A Wright
Journal:  Proc Natl Acad Sci U S A       Date:  1985-08       Impact factor: 11.205

5.  Transposition of the Lac region of E. coli.

Authors:  J R Beckwith; E R Signer; W Epstein
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1966

6.  Structural analysis of Tn5.

Authors:  E A Auerswald; G Ludwig; H Schaller
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1981

7.  Protein localization in E. coli: is there a common step in the secretion of periplasmic and outer-membrane proteins?

Authors:  K Ito; P J Bassford; J Beckwith
Journal:  Cell       Date:  1981-06       Impact factor: 41.582

8.  Deletion map of the Escherichia coli structural gene for alkaline phosphatase, phoA.

Authors:  A Sarthy; S Michaelis; J Beckwith
Journal:  J Bacteriol       Date:  1981-01       Impact factor: 3.490

9.  Secretion of beta-lactamase requires the carboxy end of the protein.

Authors:  D Koshland; D Botstein
Journal:  Cell       Date:  1980-07       Impact factor: 41.582

10.  The plasmid cloning vector pBR325 contains a 482 base-pair-long inverted duplication.

Authors:  P Prentki; F Karch; S Iida; J Meyer
Journal:  Gene       Date:  1981-09       Impact factor: 3.688
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  476 in total

1.  Expression of the putative siderophore receptor gene bfrZ is controlled by the extracytoplasmic-function sigma factor BupI in Bordetella bronchiseptica.

Authors:  E Pradel; C Locht
Journal:  J Bacteriol       Date:  2001-05       Impact factor: 3.490

Review 2.  The structural gene for microcin H47 encodes a peptide precursor with antibiotic activity.

Authors:  E Rodríguez; C Gaggero; M Laviña
Journal:  Antimicrob Agents Chemother       Date:  1999-09       Impact factor: 5.191

3.  Green fluorescent protein functions as a reporter for protein localization in Escherichia coli.

Authors:  B J Feilmeier; G Iseminger; D Schroeder; H Webber; G J Phillips
Journal:  J Bacteriol       Date:  2000-07       Impact factor: 3.490

4.  Cloning the soil metagenome: a strategy for accessing the genetic and functional diversity of uncultured microorganisms.

Authors:  M R Rondon; P R August; A D Bettermann; S F Brady; T H Grossman; M R Liles; K A Loiacono; B A Lynch; I A MacNeil; C Minor; C L Tiong; M Gilman; M S Osburne; J Clardy; J Handelsman; R M Goodman
Journal:  Appl Environ Microbiol       Date:  2000-06       Impact factor: 4.792

5.  Membrane topology of the Bacillus subtilis pro-sigma(K) processing complex.

Authors:  D H Green; S M Cutting
Journal:  J Bacteriol       Date:  2000-01       Impact factor: 3.490

6.  Functional analysis of the active partition region of the Coxiella burnetii plasmid QpH1.

Authors:  Z Lin; L P Mallavia
Journal:  J Bacteriol       Date:  1999-03       Impact factor: 3.490

7.  Protein folding in the periplasm in the absence of primary oxidant DsbA: modulation of redox potential in periplasmic space via OmpL porin.

Authors:  C Dartigalongue; H Nikaido; S Raina
Journal:  EMBO J       Date:  2000-11-15       Impact factor: 11.598

8.  In vivo and in vitro effects of (p)ppGpp on the sigma(54) promoter Pu of the TOL plasmid of Pseudomonas putida.

Authors:  M Carmona; M J Rodríguez; O Martínez-Costa; V De Lorenzo
Journal:  J Bacteriol       Date:  2000-09       Impact factor: 3.490

Review 9.  Measurement of bacterial gene expression in vivo.

Authors:  I Hautefort; J C Hinton
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2000-05-29       Impact factor: 6.237

10.  Proteolytic activity of YibP protein in Escherichia coli.

Authors:  Toshiharu Ichimura; Mitsuyoshi Yamazoe; Maki Maeda; Chieko Wada; Sota Hiraga
Journal:  J Bacteriol       Date:  2002-05       Impact factor: 3.490
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