Literature DB >> 8388893

Nested polymerase chain reaction for high-sensitivity detection of enteroviral RNA in biological samples.

G M Severini1, L Mestroni, A Falaschi, F Camerini, M Giacca.   

Abstract

A method based on nested polymerase chain reaction was developed for the detection of enteroviral genomes in biological samples. By taking advantage of the conserved 5' noncoding region of the enteroviral RNA, two sets of primers were utilized, enabling the detection either of a broad range of enteroviruses or of group B coxsackieviruses only. The sensitivity of the method is close to the detection of single molecules of viral RNA in as much as 1 mg of tissue sample. A preliminary study showed the usefulness of this technique for the analysis of endomyocardial biopsy samples from patients with idiopathic dilated cardiomyopathy and myocarditis.

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Year:  1993        PMID： 8388893      PMCID： PMC262935          DOI： 10.1128/jcm.31.5.1345-1349.1993

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  27 in total

1.  Enzymatic RNA amplification of the enteroviruses.

Authors:  H A Rotbart
Journal:  J Clin Microbiol       Date:  1990-03       Impact factor: 5.948

2.  Polymerase chain reaction for human picornaviruses.

Authors:  T Hyypiä; P Auvinen; M Maaronen
Journal:  J Gen Virol       Date:  1989-12       Impact factor: 3.891

3.  Minimal homology requirements for PCR primers.

Authors:  R Sommer; D Tautz
Journal:  Nucleic Acids Res       Date:  1989-08-25       Impact factor: 16.971

4.  Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.

Authors:  P Chomczynski; N Sacchi
Journal:  Anal Biochem       Date:  1987-04       Impact factor: 3.365

5.  Nonisotopic oligomeric probes for the human enteroviruses.

Authors:  H A Rotbart; P S Eastman; J L Ruth; K K Hirata; M J Levin
Journal:  J Clin Microbiol       Date:  1988-12       Impact factor: 5.948

6.  Limiting dilution assays for the determination of immunocompetent cell frequencies. I. Data analysis.

Authors:  C Taswell
Journal:  J Immunol       Date:  1981-04       Impact factor: 5.422

7.  The complete nucleotide sequence of coxsackievirus A21.

Authors:  P J Hughes; C North; P D Minor; G Stanway
Journal:  J Gen Virol       Date:  1989-11       Impact factor: 3.891

8.  The nucleotide sequence of coxsackievirus A9; implications for receptor binding and enterovirus classification.

Authors:  K H Chang; P Auvinen; T Hyypiä; G Stanway
Journal:  J Gen Virol       Date:  1989-12       Impact factor: 3.891

9.  Enteroviral RNA sequences detected by polymerase chain reaction in muscle of patients with postviral fatigue syndrome.

Authors:  J W Gow; W M Behan; G B Clements; C Woodall; M Riding; P O Behan
Journal:  BMJ       Date:  1991-03-23

10.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205
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  21 in total

1.  Detection and rapid differentiation of human enteroviruses following genomic amplification.

Authors:  M M Kuan
Journal:  J Clin Microbiol       Date:  1997-10       Impact factor: 5.948

2.  Antiviral effects of aqueous extract from Spatholobus suberectus Dunn. against coxsackievirus B3 in mice.

Authors:  Ji Pang; Jin-peng Guo; Min Jin; Zhi-qiang Chen; Xin-wei Wang; Jun-Wen Li
Journal:  Chin J Integr Med       Date:  2011-06-29       Impact factor: 1.978

3.  Enteroviral pharyngitis diagnosed by reverse transcriptase-polymerase chain reaction.

Authors:  M Sharland; J Hodgson; E G Davies; J Booth; S Jeffery
Journal:  Arch Dis Child       Date:  1996-05       Impact factor: 3.791

4.  Rapid diagnosis of enterovirus infection by a new one-step reverse transcription-PCR assay.

Authors:  H H Kessler; B Santner; H Rabenau; A Berger; A Vince; C Lewinski; B Weber; K Pierer; D Stuenzner; E Marth; H W Doerr
Journal:  J Clin Microbiol       Date:  1997-04       Impact factor: 5.948

5.  Surveillance of poliomyelitis in Northern Italy: Results of acute flaccid paralysis surveillance and environmental surveillance, 2012-2015.

Authors:  Laura Pellegrinelli; Laura Bubba; Valeria Primache; Elena Pariani; Andrea Battistone; Roberto Delogu; Stefano Fiore; Sandro Binda
Journal:  Hum Vaccin Immunother       Date:  2016-12-08       Impact factor: 3.452

6.  Nested PCR for rapid detection of mumps virus in cerebrospinal fluid from patients with neurological diseases.

Authors:  G P Poggio; C Rodriguez; D Cisterna; M C Freire; J Cello
Journal:  J Clin Microbiol       Date:  2000-01       Impact factor: 5.948

7.  Coxsackievirus B3-induced myocarditis. Characterization of stable attenuated variants that protect against infection with the cardiovirulent wild-type strain.

Authors:  H Zhang; P Morgan-Capner; N Latif; Y A Pandolfino; W Fan; M J Dunn; L C Archard
Journal:  Am J Pathol       Date:  1997-06       Impact factor: 4.307

8.  Evaluation of an enterovirus group-specific anti-VP1 monoclonal antibody, 5-D8/1, in comparison with neutralization and PCR for rapid identification of enteroviruses in cell culture.

Authors:  A Trabelsi; F Grattard; M Nejmeddine; M Aouni; T Bourlet; B Pozzetto
Journal:  J Clin Microbiol       Date:  1995-09       Impact factor: 5.948

9.  Absence of linkage between idiopathic dilated cardiomyopathy and candidate genes involved in the immune function in a large Italian pedigree.

Authors:  M Krajinovic; L Mestroni; G M Severini; B Pinamonti; F Camerini; A Falaschi; M Giacca
Journal:  J Med Genet       Date:  1994-10       Impact factor: 6.318

10.  Evaluation of a commercially available reverse transcription-PCR assay for diagnosis of enteroviral infection in archival and prospectively collected cerebrospinal fluid specimens.

Authors:  F Pozo; I Casas; A Tenorio; G Trallero; J M Echevarria
Journal:  J Clin Microbiol       Date:  1998-06       Impact factor: 5.948
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