Literature DB >> 2852677

Nonisotopic oligomeric probes for the human enteroviruses.

H A Rotbart1, P S Eastman, J L Ruth, K K Hirata, M J Levin.   

Abstract

Oligonucleotide probes were prepared from highly conserved regions of human enteroviral genomes. These reagents were labeled with either 32P or alkaline phosphatase and were successfully used in blot assays to detect a wide variety of human enteroviruses, proving the potential utility of oligomeric sequences as pan-enteroviral probes. The availability of nonisotopic probes will ultimately make a hybridization assay for enteroviruses easier, shorter, and more adaptable for routine diagnostic laboratories.

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Year:  1988        PMID： 2852677      PMCID： PMC266971          DOI： 10.1128/jcm.26.12.2669-2671.1988

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  12 in total

1.  Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.

Authors:  K B Mullis; F A Faloona
Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

2.  Molecular cloning and sequence determination of the genomic regions encoding protease and genome-linked protein of three picornaviruses.

Authors:  G Werner; B Rosenwirth; E Bauer; J M Seifert; F J Werner; J Besemer
Journal:  J Virol       Date:  1986-03       Impact factor: 5.103

3.  RNA target loss during solid phase hybridization of body fluids--a quantitative study.

Authors:  H A Rotbart; M J Levin; N L Murphy; M J Abzug
Journal:  Mol Cell Probes       Date:  1987-12       Impact factor: 2.365

4.  Use of subgenomic poliovirus DNA hybridization probes to detect the major subgroups of enteroviruses.

Authors:  H A Rotbart; M J Levin; L P Villarreal
Journal:  J Clin Microbiol       Date:  1984-12       Impact factor: 5.948

5.  Enzyme-linked immunosorbent assay for the detection and identification of coxsackie B antigen in tissue cultures and clinical specimens.

Authors:  R H Yolken; V Torsch
Journal:  J Med Virol       Date:  1980       Impact factor: 2.327

6.  Coxsackievirus B3: primary structure of the 5' non-coding and capsid protein-coding regions of the genome.

Authors:  S Tracy; H L Liu; N M Chapman
Journal:  Virus Res       Date:  1985-10       Impact factor: 3.303

7.  Factors involved in enzyme-linked immunoassay of viruses and evaluation of the method for identification of enteroviruses.

Authors:  J E Herrmann; R M Hendry; M F Collins
Journal:  J Clin Microbiol       Date:  1979-08       Impact factor: 5.948

8.  Preparation of oligodeoxynucleotide-alkaline phosphatase conjugates and their use as hybridization probes.

Authors:  E Jablonski; E W Moomaw; R H Tullis; J L Ruth
Journal:  Nucleic Acids Res       Date:  1986-08-11       Impact factor: 16.971

9.  The clinical relevance of 'CSF viral culture'. A two-year experience with aseptic meningitis in Rochester, NY.

Authors:  T Chonmaitree; M A Menegus; K R Powell
Journal:  JAMA       Date:  1982-04-02       Impact factor: 56.272

10.  Enzyme-linked immunosorbent assay for detection and identification of coxsackieviruses A.

Authors:  R H Yolken; V M Torsch
Journal:  Infect Immun       Date:  1981-02       Impact factor: 3.441
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  9 in total

1.  Detection of enteroviral RNA in idiopathic dilated cardiomyopathy and other human cardiac tissues.

Authors:  L M Weiss; X F Liu; K L Chang; M E Billingham
Journal:  J Clin Invest       Date:  1992-07       Impact factor: 14.808

2.  A structural model for the genome of echovirus 22.

Authors:  L A Seal; R M Jamison
Journal:  Arch Virol       Date:  1990       Impact factor: 2.574

Review 3.  Nucleic acid detection systems for enteroviruses.

Authors:  H A Rotbart
Journal:  Clin Microbiol Rev       Date:  1991-04       Impact factor: 26.132

4.  Enhancement of the AMPLICOR enterovirus PCR test with a coprecipitant.

Authors:  E W Taggart; C L Byington; D R Hillyard; J E Robison; K C Carroll
Journal:  J Clin Microbiol       Date:  1998-11       Impact factor: 5.948

5.  Molecular detection and identification of enteroviruses using enzymatic amplification and nucleic acid hybridization.

Authors:  N M Chapman; S Tracy; C J Gauntt; U Fortmueller
Journal:  J Clin Microbiol       Date:  1990-05       Impact factor: 5.948

6.  Enzymatic RNA amplification of the enteroviruses.

Authors:  H A Rotbart
Journal:  J Clin Microbiol       Date:  1990-03       Impact factor: 5.948

7.  Evaluation of an enterovirus group-specific anti-VP1 monoclonal antibody, 5-D8/1, in comparison with neutralization and PCR for rapid identification of enteroviruses in cell culture.

Authors:  A Trabelsi; F Grattard; M Nejmeddine; M Aouni; T Bourlet; B Pozzetto
Journal:  J Clin Microbiol       Date:  1995-09       Impact factor: 5.948

8.  Quantitation of enteroviral RNA by competitive polymerase chain reaction.

Authors:  T A Martino; M J Sole; L Z Penn; C C Liew; P Liu
Journal:  J Clin Microbiol       Date:  1993-10       Impact factor: 5.948

9.  Nested polymerase chain reaction for high-sensitivity detection of enteroviral RNA in biological samples.

Authors:  G M Severini; L Mestroni; A Falaschi; F Camerini; M Giacca
Journal:  J Clin Microbiol       Date:  1993-05       Impact factor: 5.948
  9 in total

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