Literature DB >> 8376351

A method for selection of mutations at the tdk locus in Escherichia coli.

W C Summers1, P Raksin.   

Abstract

Mutants of Escherichia coli which are resistant to 5-fluorodeoxyuridine all have mutations which map at a single locus at 27.5 min on the genetic map of E. coli. Extracts prepared from each mutant were deficient in thymidine kinase activity measured in vitro. Simple selective conditions which allowed detection of one mutant in the presence of 10(7) wild-type bacteria were found. These results show that loss of thymidine kinase activity is the usual mechanism for 5-fluorodeoxyuridine resistance and that all such mutations occur at the locus previously designated tdk.

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Year:  1993        PMID： 8376351      PMCID： PMC206689          DOI： 10.1128/jb.175.18.6049-6051.1993

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  10 in total

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Journal:  J Biol Chem       Date:  1956-01       Impact factor: 5.157

2.  [The biosynthesis of beta-galactosidase (lactase) in Escherichia coli; the specificity of induction].

Authors:  J MONOD; G COHEN-BAZIRE; M COHN
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3.  Purification and crystallization of thymidine kinase from herpes simplex virus type 1.

Authors:  M R Sanderson; P S Freemont; H M Murthy; J F Krane; W C Summers; T A Steitz
Journal:  J Mol Biol       Date:  1988-08-20       Impact factor: 5.469

4.  Chemically induced mutagenesis in a shuttle vector with a low-background mutant frequency.

Authors:  N R Drinkwater; D K Klinedinst
Journal:  Proc Natl Acad Sci U S A       Date:  1986-05       Impact factor: 11.205

5.  Location on the chromosome of Salmonella typhimurium of genes governing pyrimidine metabolism. II. Uridine kinase, cytosine deaminase and thymidine kinase.

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Journal:  Mol Gen Genet       Date:  1972

6.  A deoxythymidine kinase-deficient mutant of Escherichia coli. I. Isolation and some properties.

Authors:  S Hiraga; K Igarashi; T Yura
Journal:  Biochim Biophys Acta       Date:  1967-08-22

7.  Metabolism of pyrimidines and pyrimidine nucleosides by Salmonella typhimurium.

Authors:  C F Beck; J L Ingraham; J Neuhard; E Thomassen
Journal:  J Bacteriol       Date:  1972-04       Impact factor: 3.490

8.  A deoxythymidine kinase deficient mutant of Escherichia coli. II. Mapping and transduction studies with phage phi 80.

Authors:  K Igarashi; S Hiraga; T Yura
Journal:  Genetics       Date:  1967-11       Impact factor: 4.562

9.  A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli.

Authors:  M Singer; T A Baker; G Schnitzler; S M Deischel; M Goel; W Dove; K J Jaacks; A D Grossman; J W Erickson; C A Gross
Journal:  Microbiol Rev       Date:  1989-03

10.  Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes.

Authors:  F W Studier; B A Moffatt
Journal:  J Mol Biol       Date:  1986-05-05       Impact factor: 5.469
  10 in total
  14 in total

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Authors:  Samantha S Orchard; Heidi Goodrich-Blair
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2.  Streptococcus gordonii strains resistant to fluorodeoxyuridine contain mutations in the thymidine kinase gene and are deficient in thymidine kinase activity.

Authors:  C A Franke; T M Bolman; S A Ottum; K F Jones; D E Hruby
Journal:  Antimicrob Agents Chemother       Date:  2000-03       Impact factor: 5.191

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Authors:  M E Black; T G Newcomb; H M Wilson; L A Loeb
Journal:  Proc Natl Acad Sci U S A       Date:  1996-04-16       Impact factor: 11.205

4.  Substrate specificity of Epstein-Barr virus thymidine kinase.

Authors:  P P Tung; W C Summers
Journal:  Antimicrob Agents Chemother       Date:  1994-09       Impact factor: 5.191

5.  Quantitative determination of uracil residues in Escherichia coli DNA: Contribution of ung, dug, and dut genes to uracil avoidance.

Authors:  Sibghat-Ullah Lari; Cheng-Yao Chen; Béata G Vertéssy; Jeff Morré; Samuel E Bennett
Journal:  DNA Repair (Amst)       Date:  2006-08-14

6.  A rapid phenotypic assay for detection of acyclovir-resistant varicella-zoster virus with mutations in the thymidine kinase open reading frame.

Authors:  R Sahli; G Andrei; C Estrade; R Snoeck; P R Meylan
Journal:  Antimicrob Agents Chemother       Date:  2000-04       Impact factor: 5.191

7.  Escherichia coli DNA polymerase IV contributes to spontaneous mutagenesis at coding sequences but not microsatellite alleles.

Authors:  Kimberly D Jacob; Kristin A Eckert
Journal:  Mutat Res       Date:  2007-03-02       Impact factor: 2.433

8.  The phtC-phtD locus equips Legionella pneumophila for thymidine salvage and replication in macrophages.

Authors:  Maris V Fonseca; John-Demian Sauer; Sebastien Crepin; Brenda Byrne; Michele S Swanson
Journal:  Infect Immun       Date:  2013-12-02       Impact factor: 3.441

9.  A nucleoside kinase as a dual selector for genetic switches and circuits.

Authors:  Yohei Tashiro; Hiroki Fukutomi; Kei Terakubo; Kyoichi Saito; Daisuke Umeno
Journal:  Nucleic Acids Res       Date:  2010-11-09       Impact factor: 16.971

10.  Extension of lifespan in C. elegans by naphthoquinones that act through stress hormesis mechanisms.

Authors:  Piper R Hunt; Tae Gen Son; Mark A Wilson; Quian-Sheng Yu; William H Wood; Yongqing Zhang; Kevin G Becker; Nigel H Greig; Mark P Mattson; Simonetta Camandola; Catherine A Wolkow
Journal:  PLoS One       Date:  2011-07-13       Impact factor: 3.240
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