Literature DB >> 2845104

Purification and crystallization of thymidine kinase from herpes simplex virus type 1.

M R Sanderson1, P S Freemont, H M Murthy, J F Krane, W C Summers, T A Steitz.   

Abstract

Thymidine kinase from herpes simplex virus type 1 (ATP:thymidine 5'-phosphotransferase; EC 2.7.1.21) has been purified from an overexpression system and crystallized against ammonium sulfate by using the hanging-drop technique. The tetragonal crystals are of space group P4122 or P4322, and have unit cell dimensions a = b = 84 A, c = 180 A.

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Year:  1988        PMID： 2845104     DOI： 10.1016/0022-2836(88)90569-4

Source DB:  PubMed          Journal:  J Mol Biol        ISSN： 0022-2836            Impact factor:   5.469


  3 in total

1.  Thymidine kinase mutants obtained by random sequence selection.

Authors:  K M Munir; D C French; L A Loeb
Journal:  Proc Natl Acad Sci U S A       Date:  1993-05-01       Impact factor: 11.205

2.  A method for selection of mutations at the tdk locus in Escherichia coli.

Authors:  W C Summers; P Raksin
Journal:  J Bacteriol       Date:  1993-09       Impact factor: 3.490

3.  3'-Amino thymidine affinity matrix for the purification of herpes simplex virus thymidine kinase.

Authors:  P P Tung; J Respass; W C Summers
Journal:  Yale J Biol Med       Date:  1996 Nov-Dec
  3 in total

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