Literature DB >> 8368859

Detection of Listeria monocytogenes with a nonisotopic polymerase chain reaction-coupled ligase chain reaction assay.

M Wiedmann1, F Barany, C A Batt.   

Abstract

A polymerase chain reaction (PCR)-coupled ligase chain reaction (LCR) assay for the specific detection of Listeria monocytogenes (M. Wiedmann, J. Czajka, F. Barany, and C. A. Batt, Appl. Environ. Microbiol. 58:3443-3447, 1992) has been modified for detection of the LCR products with a nonisotopic readout. When a chemiluminescent or a colorimetric substrate for the nonisotopic detection of the LCR products was used, the PCR-coupled LCR gave a sensitivity of 10 CFU of L. monocytogenes. The detection method with the chemiluminescent substrate Lumi-Phos 530 permitted detection of the LCR products in less than 3 h, so that the whole assay can be completed within 10 h.

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Year:  1993        PMID： 8368859      PMCID： PMC182352          DOI： 10.1128/aem.59.8.2743-2745.1993

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  19 in total

1.  The homologous and heterologous regions within the iap gene allow genus- and species-specific identification of Listeria spp. by polymerase chain reaction.

Authors:  A Bubert; S Köhler; W Goebel
Journal:  Appl Environ Microbiol       Date:  1992-08       Impact factor: 4.792

2.  Automated DNA diagnostics using an ELISA-based oligonucleotide ligation assay.

Authors:  D A Nickerson; R Kaiser; S Lappin; J Stewart; L Hood; U Landegren
Journal:  Proc Natl Acad Sci U S A       Date:  1990-11       Impact factor: 11.205

3.  Development of a 16S rRNA-based oligomer probe specific for Listeria monocytogenes.

Authors:  R F Wang; W W Cao; M G Johnson
Journal:  Appl Environ Microbiol       Date:  1991-12       Impact factor: 4.792

4.  Use of polymerase chain reaction for detection of Listeria monocytogenes in food.

Authors:  C Niederhauser; U Candrian; C Höfelein; M Jermini; H P Bühler; J Lüthy
Journal:  Appl Environ Microbiol       Date:  1992-05       Impact factor: 4.792

5.  Sensitive and specific detection of Listeria monocytogenes in milk and ground beef with the polymerase chain reaction.

Authors:  E J Thomas; R K King; J Burchak; V P Gannon
Journal:  Appl Environ Microbiol       Date:  1991-09       Impact factor: 4.792

6.  Differentiation of Listeria monocytogenes and Listeria innocua by 16S rRNA genes and intraspecies discrimination of Listeria monocytogenes strains by random amplified polymorphic DNA polymorphisms.

Authors:  J Czajka; N Bsat; M Piani; W Russ; K Sultana; M Wiedmann; R Whitaker; C A Batt
Journal:  Appl Environ Microbiol       Date:  1993-01       Impact factor: 4.792

7.  A combined PCR and selective enrichment method for rapid detection of Listeria monocytogenes.

Authors:  S Fitter; M Heuzenroeder; C J Thomas
Journal:  J Appl Bacteriol       Date:  1992-07

8.  Discrimination of Listeria monocytogenes from other Listeria species by ligase chain reaction.

Authors:  M Wiedmann; J Czajka; F Barany; C A Batt
Journal:  Appl Environ Microbiol       Date:  1992-11       Impact factor: 4.792

9.  Detection of Listeria species and Listeria monocytogenes using polymerase chain reaction.

Authors:  P M Border; J J Howard; G S Plastow; K W Siggens
Journal:  Lett Appl Microbiol       Date:  1990-09       Impact factor: 2.858

10.  16S rRNA-based probes and polymerase chain reaction method to detect Listeria monocytogenes cells added to foods.

Authors:  R F Wang; W W Cao; M G Johnson
Journal:  Appl Environ Microbiol       Date:  1992-09       Impact factor: 4.792
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  12 in total

1.  Detection of viable Listeria monocytogenes with a 5' nuclease PCR assay.

Authors:  D M Norton; C A Batt
Journal:  Appl Environ Microbiol       Date:  1999-05       Impact factor: 4.792

2.  A quantitative assay for assessing allelic proportions by iterative gap ligation.

Authors:  J Stewart; P Kozlowski; M Sowden; E Messing; H C Smith
Journal:  Nucleic Acids Res       Date:  1998-02-15       Impact factor: 16.971

3.  Combinatorial library diversity: probability assessment of library populations.

Authors:  B Ward; T Juehne
Journal:  Nucleic Acids Res       Date:  1998-02-15       Impact factor: 16.971

4.  Improving the fidelity of Thermus thermophilus DNA ligase.

Authors:  J Luo; D E Bergstrom; F Barany
Journal:  Nucleic Acids Res       Date:  1996-08-01       Impact factor: 16.971

Review 5.  Impact of molecular biology on the detection of foodborne pathogens.

Authors:  P Feng
Journal:  Mol Biotechnol       Date:  1997-06       Impact factor: 2.695

6.  Identification of Erwinia stewartii by a ligase chain reaction assay.

Authors:  W J Wilson; M Wiedmann; H R Dillard; C A Batt
Journal:  Appl Environ Microbiol       Date:  1994-01       Impact factor: 4.792

7.  Diagnosis and epidemiological association of Listeria monocytogenes strains in two outbreaks of listerial encephalitis in small ruminants.

Authors:  M Wiedmann; J Czajka; N Bsat; M Bodis; M C Smith; T J Divers; C A Batt
Journal:  J Clin Microbiol       Date:  1994-04       Impact factor: 5.948

8.  Detection of point mutations with a modified ligase chain reaction (Gap-LCR).

Authors:  K Abravaya; J J Carrino; S Muldoon; H H Lee
Journal:  Nucleic Acids Res       Date:  1995-02-25       Impact factor: 16.971

9.  Use of a fluorogenic probe in a PCR-based assay for the detection of Listeria monocytogenes.

Authors:  H A Bassler; S J Flood; K J Livak; J Marmaro; R Knorr; C A Batt
Journal:  Appl Environ Microbiol       Date:  1995-10       Impact factor: 4.792

10.  Exploring specific primers targeted against different genes for a multiplex PCR for detection of Listeria monocytogenes.

Authors:  Ashwani Kumar; Sunita Grover; Virender Kumar Batish
Journal:  3 Biotech       Date:  2014-05-21       Impact factor: 2.406
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