| Literature DB >> 1512178 |
S Fitter1, M Heuzenroeder, C J Thomas.
Abstract
Development of a routine detection assay for Listeria monocytogenes in foods that uses the polymerase chain reaction (PCR) and enrichment cultures was investigated. Oligonucleotide primers were chosen to amplify a 3' region of L. monocytogenes hlyA gene spanning a conserved HindIII site. PCR detection sensitivity for L. monocytogenes in dilutions of pure enrichment cultures was between 50 and 500 colony forming units. A short enrichment period before PCR amplification allowed detection of the organisms in a range of complex foods contaminated with 10(4) cfu/g. Detection sensitivity for the assay in the presence of chicken skin and soft cheese was determined at 10-100 cfu/g. Utilization of enrichment cultures and PCR allowed identification of the organism within 24 h or 2 days.Entities:
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Year: 1992 PMID： 1512178 DOI： 10.1111/j.1365-2672.1992.tb04968.x
Source DB: PubMed Journal: J Appl Bacteriol ISSN： 0021-8847