Literature DB >> 8358827

High efficiency transformation of Kluyveromyces marxianus by a replicative plasmid.

F Iborra1.   

Abstract

Kluyveromyces marxianus can be transformed with an efficiency of 10(5) transformants/microgram of DNA by a replicative plasmid using electroporation. In order to obtain this efficiency, we isolated ura- mutants cells which can be complemented by the URA3 gene from Saccharomyces cerevisiae. The URA3 gene and KARS2, a replicative origin from Kluyveromyces lactis which functions in K. marxianus, were ligated together in a plasmid which can be used as a vector to transform this strain.

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Year:  1993        PMID： 8358827     DOI： 10.1007/bf00324685

Source DB:  PubMed          Journal:  Curr Genet        ISSN： 0172-8083            Impact factor:   3.886


  11 in total

1.  Coordination of the synthesis of the enzymes in the pyrimidine pathway of E. coli.

Authors:  J R BECKWITH; A B PARDEE; R AUSTRIAN; F JACOB
Journal:  J Mol Biol       Date:  1962-12       Impact factor: 5.469

2.  High-efficiency transformation of yeast by electroporation.

Authors:  D M Becker; L Guarente
Journal:  Methods Enzymol       Date:  1991       Impact factor: 1.600

3.  High efficiency transformation of Schizosaccharomyces pombe by electroporation.

Authors:  H L Prentice
Journal:  Nucleic Acids Res       Date:  1992-02-11       Impact factor: 16.971

4.  High frequency of yeast transformation by plasmids carrying part or entire 2-micron yeast plasmid.

Authors:  C Gerbaud; P Fournier; H Blanc; M Aigle; H Heslot; M Guerineau
Journal:  Gene       Date:  1979-03       Impact factor: 3.688

5.  Transformation of Kluyveromyces fragilis.

Authors:  S Das; E Kellermann; C P Hollenberg
Journal:  J Bacteriol       Date:  1984-06       Impact factor: 3.490

6.  Cloning and expression of the structural gene for beta-glucosidase of Kluyveromyces fragilis in Escherichia coli and Saccharomyces cerevisiae.

Authors:  A Raynal; M Guerineau
Journal:  Mol Gen Genet       Date:  1984

7.  Regulation of pyrimidine biosynthesis in Saccharomyces cerevisiae.

Authors:  F Lacroute
Journal:  J Bacteriol       Date:  1968-03       Impact factor: 3.490

8.  High efficiency transformation of intact yeast cells by electric field pulses.

Authors:  E Meilhoc; J M Masson; J Teissié
Journal:  Biotechnology (N Y)       Date:  1990-03

9.  A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance.

Authors:  J D Boeke; F LaCroute; G R Fink
Journal:  Mol Gen Genet       Date:  1984

10.  A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

Authors:  R S Sikorski; P Hieter
Journal:  Genetics       Date:  1989-05       Impact factor: 4.562
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  3 in total

1.  High-efficiency electrotransformation of the yeast Schwanniomyces occidentalis.

Authors:  P Costaglioli; E Meilhoc; J M Masson
Journal:  Curr Genet       Date:  1994-12       Impact factor: 3.886

2.  High-temperature ethanol fermentation and transformation with linear DNA in the thermotolerant yeast Kluyveromyces marxianus DMKU3-1042.

Authors:  Sanom Nonklang; Babiker M A Abdel-Banat; Kamonchai Cha-aim; Nareerat Moonjai; Hisashi Hoshida; Savitree Limtong; Mamoru Yamada; Rinji Akada
Journal:  Appl Environ Microbiol       Date:  2008-10-17       Impact factor: 4.792

3.  Thermal adaptability of Kluyveromyces marxianus in recombinant protein production.

Authors:  Stefano Raimondi; Elena Zanni; Alberto Amaretti; Claudio Palleschi; Daniela Uccelletti; Maddalena Rossi
Journal:  Microb Cell Fact       Date:  2013-04-15       Impact factor: 5.328
  3 in total

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