Literature DB >> 1366502

High efficiency transformation of intact yeast cells by electric field pulses.

E Meilhoc1, J M Masson, J Teissié.   

Abstract

We have developed an efficient method that electrically introduces DNA into intact yeast cells. Saccharomyces cerevisiae was used as a model in order to optimize the transformation protocol. Transformation efficiencies of 10(7) transformants/micrograms of plasmid DNA were obtained with a square wave electric pulse of 2.7 kV/cm during 15 milliseconds. The technique is simple and rapid. Even small quantities of DNA (100 pg) can be used to transform 10(8) cells. Important parameters are the pulse field strength and duration. Pretreatment of the yeast cells in the early phase of exponential growth with dithiothreitol increases transformation efficiency. The method has been successfully applied to various strains of S. cerevisiae as well as to other types of yeast.

Entities:  

Mesh：

Substances：

Year:  1990        PMID： 1366502     DOI： 10.1038/nbt0390-223

Source DB:  PubMed          Journal:  Biotechnology (N Y)        ISSN： 0733-222X


  43 in total

1.  Electroporation and electrophoretic DNA transfer into cells. The effect of DNA interaction with electropores.

Authors:  S I Sukharev; V A Klenchin; S M Serov; L V Chernomordik
Journal:  Biophys J       Date:  1992-11       Impact factor: 4.033

2.  Shuffled antibody libraries created by in vivo homologous recombination and yeast surface display.

Authors:  Jeffrey S Swers; Brenda A Kellogg; K Dane Wittrup
Journal:  Nucleic Acids Res       Date:  2004-02-20       Impact factor: 16.971

3.  Electroporation by using bipolar oscillating electric field: an improved method for DNA transfection of NIH 3T3 cells.

Authors:  E Tekle; R D Astumian; P B Chock
Journal:  Proc Natl Acad Sci U S A       Date:  1991-05-15       Impact factor: 11.205

4.  Transformation of Kluyveromyces lactis by Electroporation.

Authors:  M Sánchez; F J Iglesias; C Santamaría; A Domínguez
Journal:  Appl Environ Microbiol       Date:  1993-07       Impact factor: 4.792

5.  One-step transformation of yeast in stationary phase.

Authors:  D C Chen; B C Yang; T T Kuo
Journal:  Curr Genet       Date:  1992-01       Impact factor: 3.886

6.  High efficiency transformation of Schizosaccharomyces pombe by electroporation.

Authors:  H L Prentice
Journal:  Nucleic Acids Res       Date:  1992-02-11       Impact factor: 16.971

7.  Two functionally distinct domains generated by in vivo cleavage of Nup145p: a novel biogenesis pathway for nucleoporins.

Authors:  M T Teixeira; S Siniossoglou; S Podtelejnikov; J C Bénichou; M Mann; B Dujon; E Hurt; E Fabre
Journal:  EMBO J       Date:  1997-08-15       Impact factor: 11.598

8.  Parameters affecting lithium acetate-mediated transformation of Saccharomyces cerevisiae and development of a rapid and simplified procedure.

Authors:  R Soni; J P Carmichael; J A Murray
Journal:  Curr Genet       Date:  1993-11       Impact factor: 3.886

9.  Calcium-mediated DNA adsorption to yeast cells and kinetics of cell transformation by electroporation.

Authors:  E Neumann; S Kakorin; I Tsoneva; B Nikolova; T Tomov
Journal:  Biophys J       Date:  1996-08       Impact factor: 4.033

10.  The hexokinase gene is required for transcriptional regulation of the glucose transporter gene RAG1 in Kluyveromyces lactis.

Authors:  C Prior; P Mamessier; H Fukuhara; X J Chen; M Wesolowski-Louvel
Journal:  Mol Cell Biol       Date:  1993-07       Impact factor: 4.272
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.