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Literature DB >> 8349571

Immunocytochemical analysis of poly-beta-hydroxybutyrate (PHB) synthase in Alcaligenes eutrophus H16: localization of the synthase enzyme at the surface of PHB granules.

T U Gerngross¹, P Reilly, J Stubbe, A J Sinskey, O P Peoples.

Abstract

Antibodies raised against the Alcaligenes eutrophus poly-beta-hydroxybutyrate (PHB) synthase polypeptide were used for immunocytochemical localization of the synthase enzyme in whole cells and purified PHB granules. The data presented demonstrate for the first time that the synthase enzyme is located on the surface of the PHB granule rather than being incorporated inside the granule during its formation. From these basic observations and data from the recent literature, a model of granule assembly is proposed.

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Year: 1993 PMID： 8349571 PMCID： PMC205001 DOI： 10.1128/jb.175.16.5289-5293.1993

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

22 in total

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21 in total

1. The Puzzling Conservation and Diversification of Lipid Droplets from Bacteria to Eukaryotes.

Authors: Josselin Lupette; Eric Maréchal
Journal: Results Probl Cell Differ Date: 2020

Review 2. Polyhydroxyalkanoate granules are complex subcellular organelles (carbonosomes).

Authors: Dieter Jendrossek
Journal: J Bacteriol Date: 2009-03-06 Impact factor: 3.490

3. Polyhydroxyalkanoate inclusion body-associated proteins and coding region in Bacillus megaterium.

Authors: G J McCool; M C Cannon
Journal: J Bacteriol Date: 1999-01 Impact factor: 3.490

4. Development of a transferable bimolecular fluorescence complementation system for the investigation of interactions between poly(3-hydroxybutyrate) granule-associated proteins in Gram-negative bacteria.

Authors: Daniel Pfeiffer; Dieter Jendrossek
Journal: Appl Environ Microbiol Date: 2013-02-22 Impact factor: 4.792

5. Purification of polyhydroxybutyrate synthase from its native organism, Ralstonia eutropha: implications for the initiation and elongation of polymer formation in vivo.

Authors: Mimi Cho; Christopher J Brigham; Anthony J Sinskey; JoAnne Stubbe
Journal: Biochemistry Date: 2012-03-07 Impact factor: 3.162

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Authors: Jiamin Tian; Anthony J Sinskey; Joanne Stubbe
Journal: J Bacteriol Date: 2005-06 Impact factor: 3.490

9. Analysis of transient polyhydroxybutyrate production in Wautersia eutropha H16 by quantitative Western analysis and transmission electron microscopy.

Authors: Jiamin Tian; Aimin He; Adam G Lawrence; Pinghua Liu; Nicki Watson; Anthony J Sinskey; Joanne Stubbe
Journal: J Bacteriol Date: 2005-06 Impact factor: 3.490

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Authors: Keiichi Uchino; Terumi Saito; Birgit Gebauer; Dieter Jendrossek
Journal: J Bacteriol Date: 2007-08-24 Impact factor: 3.490