Literature DB >> 8300240

Amino acids in pneumolysin important for hemolytic activity identified by random mutagenesis.

J Hill1, P W Andrew, T J Mitchell.   

Abstract

The structural gene of pneumolysin was randomly mutagenized. A screen, developed to identify mutants with reduced hemolytic activity, identified substitutions within the cysteine-containing region and also at residues toward the N terminus of the toxin. These are the first reported changes within the N-terminal region of the toxin that affect cytolytic activity.

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Year:  1994        PMID： 8300240      PMCID： PMC186175          DOI： 10.1128/iai.62.2.757-758.1994

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  12 in total

1.  Expression of the pneumolysin gene in Escherichia coli: rapid purification and biological properties.

Authors:  T J Mitchell; J A Walker; F K Saunders; P W Andrew; G J Boulnois
Journal:  Biochim Biophys Acta       Date:  1989-01-23

2.  CLUSTAL V: improved software for multiple sequence alignment.

Authors:  D G Higgins; A J Bleasby; R Fuchs
Journal:  Comput Appl Biosci       Date:  1992-04

3.  Identification of the structural gene encoding the SH-activated hemolysin of Listeria monocytogenes: listeriolysin O is homologous to streptolysin O and pneumolysin.

Authors:  J Mengaud; J Chenevert; C Geoffroy; J L Gaillard; P Cossart
Journal:  Infect Immun       Date:  1987-12       Impact factor: 3.441

4.  Pneumolysin, the thiol-activated toxin of Streptococcus pneumoniae, does not require a thiol group for in vitro activity.

Authors:  F K Saunders; T J Mitchell; J A Walker; P W Andrew; G J Boulnois
Journal:  Infect Immun       Date:  1989-08       Impact factor: 3.441

5.  A new method for random mutagenesis of complete genes: enzymatic generation of mutant libraries in vitro.

Authors:  P M Lehtovaara; A K Koivula; J Bamford; J K Knowles
Journal:  Protein Eng       Date:  1988-04

6.  Nucleotide sequence of the streptolysin O (SLO) gene: structural homologies between SLO and other membrane-damaging, thiol-activated toxins.

Authors:  M A Kehoe; L Miller; J A Walker; G J Boulnois
Journal:  Infect Immun       Date:  1987-12       Impact factor: 3.441

7.  Site-directed mutagenesis of the hole-forming toxin aerolysin: studies on the roles of histidines in receptor binding and oligomerization of the monomer.

Authors:  M J Green; J T Buckley
Journal:  Biochemistry       Date:  1990-02-27       Impact factor: 3.162

8.  Properties of purified pneumococcal hemolysin.

Authors:  M K Johnson
Journal:  Infect Immun       Date:  1972-11       Impact factor: 3.441

9.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

10.  Attenuated mutants of the intracellular bacterium Listeria monocytogenes obtained by single amino acid substitutions in listeriolysin O.

Authors:  E Michel; K A Reich; R Favier; P Berche; P Cossart
Journal:  Mol Microbiol       Date:  1990-12       Impact factor: 3.501
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  5 in total

1.  THP-1 monocytes up-regulate intercellular adhesion molecule 1 in response to pneumolysin from Streptococcus pneumoniae.

Authors:  Justin Thornton; Larry S McDaniel
Journal:  Infect Immun       Date:  2005-10       Impact factor: 3.441

Review 2.  Pneumococcal virulence factors: structure and function.

Authors:  M J Jedrzejas
Journal:  Microbiol Mol Biol Rev       Date:  2001-06       Impact factor: 11.056

Review 3.  The role of pneumolysin in pneumococcal pneumonia and meningitis.

Authors:  R A Hirst; A Kadioglu; C O'callaghan; P W Andrew
Journal:  Clin Exp Immunol       Date:  2004-11       Impact factor: 4.330

Review 4.  Single nucleotide variations: biological impact and theoretical interpretation.

Authors:  Panagiotis Katsonis; Amanda Koire; Stephen Joseph Wilson; Teng-Kuei Hsu; Rhonald C Lua; Angela Dawn Wilkins; Olivier Lichtarge
Journal:  Protein Sci       Date:  2014-10-20       Impact factor: 6.725

5.  Formation of pre-pore complexes of pneumolysin is accompanied by a decrease in short-range order of lipid molecules throughout vesicle bilayers.

Authors:  Bayan H A Faraj; Liam Collard; Rachel Cliffe; Leanne A Blount; Rana Lonnen; Russell Wallis; Peter W Andrew; Andrew J Hudson
Journal:  Sci Rep       Date:  2020-03-12       Impact factor: 4.379
  5 in total

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