Literature DB >> 823530

Selection and some properties of recombinant clones of lambda bacteriophage containing genes of Drosophila melanogaster.

Y V Ilyin, N A Tchurikov.   

Abstract

The lambdagt clones containing fragments of the Drosophila melanogaster genome were prepared and characterized by hybridization of their DNA with (I) lambdagt-cRNA; (2) lambdaC-cRNA; (3) Dm-cRNA; (4) the mRNA of D.melanogaster culture cells and (5) the stable cytoplasmic poly (A) RNA from the same source. The technique for a simple selection of hybrid clones is described. The hybridization with mRNA allows one to select the clones containing structural genes of D.melanogaster. It was found that in all cases when the clone contains the structural gene it also contains the reiterated base sequences of the D.melanogaster genome. Several clones containing D. melanogaster DNA fragments with a size of (2-4)x1O6 daltons hybridizing with a relatively large portion of mRNA were selected for further analysis.

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Year:  1976        PMID： 823530      PMCID： PMC343066          DOI： 10.1093/nar/3.8.2115

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  13 in total

Review 1.  DNA of Drosophila chromosomes.

Authors:  C D Laird
Journal:  Annu Rev Genet       Date:  1973       Impact factor: 16.830

2.  Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

Authors:  P A Sharp; B Sugden; J Sambrook
Journal:  Biochemistry       Date:  1973-07-31       Impact factor: 3.162

3.  Cleavage of DNA by R 1 restriction endonuclease generates cohesive ends.

Authors:  J E Mertz; R W Davis
Journal:  Proc Natl Acad Sci U S A       Date:  1972-11       Impact factor: 11.205

4.  On the structural organization of the transcriptional unit in animal chromosomes.

Authors:  G P Georgiev; A J Varshavsky; A P Ryskov; R B Church
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1974

5.  Calcium-dependent bacteriophage DNA infection.

Authors:  M Mandel; A Higa
Journal:  J Mol Biol       Date:  1970-10-14       Impact factor: 5.469

6.  Restriction of nonglucosylated T-even bacteriophages by prophage P1.

Authors:  H R Revel; C P Georgopoulos
Journal:  Virology       Date:  1969-09       Impact factor: 3.616

7.  In vitro construction of bacteriophage lambda carrying segments of the Escherichia coli chromosome: selection of hybrids containing the gene for DNA ligase.

Authors:  J R Cameron; S M Panasenko; I R Lehman; R W Davis
Journal:  Proc Natl Acad Sci U S A       Date:  1975-09       Impact factor: 11.205

8.  A system for mapping DNA sequences in the chromosomes of Drosophila melanogaster.

Authors:  P C Wensink; D J Finnegan; J E Donelson; D S Hogness
Journal:  Cell       Date:  1974-12       Impact factor: 41.582

9.  Interspersion of repetitive and nonrepetitive DNA sequences in the Drosophila melanogaster genome.

Authors:  J E Manning; C W Schmid; N Davidson
Journal:  Cell       Date:  1975-02       Impact factor: 41.582

10.  Replication and transcription of eukaryotic DNA in Escherichia coli.

Authors:  J F Morrow; S N Cohen; A C Chang; H W Boyer; H M Goodman; R B Helling
Journal:  Proc Natl Acad Sci U S A       Date:  1974-05       Impact factor: 11.205
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  8 in total

1.  Screening of cloned recombinant DNA in bacteria by in situ colony hybridization.

Authors:  B Cami; P Kourilsky
Journal:  Nucleic Acids Res       Date:  1978-07       Impact factor: 16.971

2.  Increase in the number of histone genes in case of their deficiency in Drosophila melanogaster.

Authors:  A I Chernyshev; V N Bashkirov; B A Leibovitch; R B Khesin
Journal:  Mol Gen Genet       Date:  1980

3.  Circular copies of mobile dispersed genetic elements in cultured Drosophila melanogaster cells.

Authors:  Y V Ilyin; N G Schuppe; N V Lyubomirskaya; T V Gorelova; I R Arkhipova
Journal:  Nucleic Acids Res       Date:  1984-10-11       Impact factor: 16.971

4.  Reiterated genes with varying location in intercalary heterochromatin regions of Drosophila melanogaster polytene chromosomes.

Authors:  E V Ananiev; V A Gvozdev; V Ilyin Yu; N A Tchurikov; G P Georgiev
Journal:  Chromosoma       Date:  1978-12-21       Impact factor: 4.316

5.  Transposition of mobile genetic elements in interspecific hybrids of Drosophila.

Authors:  M B Evgen'ev; G N Yenikolopov; N I Peunova; Y V Ilyin
Journal:  Chromosoma       Date:  1982       Impact factor: 4.316

6.  Isolation and characterization of a new family of mobile dispersed genetic elements, mdg3, in Drosophila melanogaster.

Authors:  Y V Ilyin; V G Chmeliauskaite; E V Ananiev; G P Georgiev
Journal:  Chromosoma       Date:  1980       Impact factor: 4.316

7.  Double-stranded sequences in RNA of Drosophila melanogaster: relation to mobile dispersed genes.

Authors:  Y V Ilyin; V G Chmeliauskaite; G P Georgiev
Journal:  Nucleic Acids Res       Date:  1980-08-11       Impact factor: 16.971

8.  Reverse transcription of Drosophila mobile dispersed genetic element RNAs: detection of intermediate forms.

Authors:  I R Arkhipova; T V Gorelova; Y V Ilyin; N G Schuppe
Journal:  Nucleic Acids Res       Date:  1984-10-11       Impact factor: 16.971
  8 in total

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