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Literature DB >> 6254733

Isolation and characterization of a new family of mobile dispersed genetic elements, mdg3, in Drosophila melanogaster.

Y V Ilyin, V G Chmeliauskaite, E V Ananiev, G P Georgiev.

Abstract

The properties of a new family of mobile dispersed genetic elements, mdg3, are described. Mdg3 is represented by a DNA fragment of about 5.5 kb long which is framed with two repetitive sequences 300-500 base pairs in length. Virtually the whole region of mdg3 including the repetitive sequences is transcribed and, as the result, 26S poly(A)+RNA is formed. The repetitive sequence is present at least at the 3'-end of a major 26S transcript. Both strands of mdg3 are transcribed, but one direction is predominant. Besides 26S RNA, a minor transcript, 15S poly(A)+RNA, can be observed. 15S RNA dose not contain sequences corresponding to the middle of mdg3. Possibly it is formed from 26S RNA by means of splicing. About 15 copies of mdg3 are present in the genome of embryonic cells and polytene chromosomes. Different copies of mdg3 are identical but surrounded with different DNA sequences. In culture cells, the gene is amplified and the number of copies is increased approximately 13 times. Mdg3 is localized in 15-17 sites on different chromosomes of D. melanogaster. Its location varies from one animal to another. The properties and possible nature of mobile dispersed genetic elements are discussed.

Entities: Species

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Year: 1980 PMID： 6254733 DOI： 10.1007/bf00292421

Source DB: PubMed Journal: Chromosoma ISSN： 0009-5915 Impact factor: 4.316

32 in total

1. A freeze-squeeze method for recovering long DNA from agarose gels.

Authors: R W Thuring; J P Sanders; P Borst
Journal: Anal Biochem Date: 1975-05-26 Impact factor: 3.365

2. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors: P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal: J Mol Biol Date: 1977-06-15 Impact factor: 5.469

3. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

Authors: P A Sharp; B Sugden; J Sambrook
Journal: Biochemistry Date: 1973-07-31 Impact factor: 3.162

4. Double-stranded regions in heterogeneous nuclear RNA from Hela cells.

Authors: W Jelinek; J E Darnell
Journal: Proc Natl Acad Sci U S A Date: 1972-09 Impact factor: 11.205

5. A membrane-filter technique for the detection of complementary DNA.

Authors: D T Denhardt
Journal: Biochem Biophys Res Commun Date: 1966-06-13 Impact factor: 3.575

6. Transposition of elements of the 412, copia and 297 dispersed repeated gene families in Drosophila.

Authors: S S Potter; W J Brorein; P Dunsmuir; G M Rubin
Journal: Cell Date: 1979-06 Impact factor: 41.582

7. Selection and some properties of recombinant clones of lambda bacteriophage containing genes of Drosophila melanogaster.

Authors: Y V Ilyin; N A Tchurikov
Journal: Nucleic Acids Res Date: 1976-08 Impact factor: 16.971

8. Evidence for transposition of dispersed repetitive DNA families in yeast.

Authors: J R Cameron; E Y Loh; R W Davis
Journal: Cell Date: 1979-04 Impact factor: 41.582

9. The transposable element Mdg3 in Drosophila melanogaster is flanked with the perfect direct and mismatched inverted repeats.

Authors: A A Bayev; A S Krayev; N V Lyubomirskaya; Y V Ilyin; K G Skryabin; G P Georgiev
Journal: Nucleic Acids Res Date: 1980-08-11 Impact factor: 16.971

10. Molecular studies on interspersed repetitive and unique sequences in the region of the complementation group uncoordinated on the X chromosome of Drosophila melanogaster.

Authors: M J Healy; R J Russell; G L Miklos
Journal: Mol Gen Genet Date: 1988-07

Isolation and characterization of a new family of mobile dispersed genetic elements, mdg3, in Drosophila melanogaster.

1. A freeze-squeeze method for recovering long DNA from agarose gels.

2. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

3. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

4. Double-stranded regions in heterogeneous nuclear RNA from Hela cells.

5. A membrane-filter technique for the detection of complementary DNA.

6. Transposition of elements of the 412, copia and 297 dispersed repeated gene families in Drosophila.

7. Selection and some properties of recombinant clones of lambda bacteriophage containing genes of Drosophila melanogaster.

8. Evidence for transposition of dispersed repetitive DNA families in yeast.

9. The transposable element Mdg3 in Drosophila melanogaster is flanked with the perfect direct and mismatched inverted repeats.

10. Double-stranded sequences in RNA of Drosophila melanogaster: relation to mobile dispersed genes.

1. Two non-gypsy rudimentary mutations and their suppression by mutations of suppressor of Hairy-wing in Drosophila.

2. Molecular analysis of the lethal(1)B214 region at the base of the X chromosome of Drosophila melanogaster.

3. 1731, a new retrotransposon with hormone modulated expression.

4. The 5' termini of RNAs encoded by the transposable element copia.

5. Transpositions of mobile dispersed genes in Drosophila melanogaster and fitness of stocks.

6. Mobile dispersed genetic elements and their possible relation to carcinogenesis.

7. Localization of tRNA genes of Drosophila melanogaster by in situ hybridization.

8. Identification of chromosome inheritance modifiers in Drosophila melanogaster.

9. Transposition of mobile genetic elements in interspecific hybrids of Drosophila.

10. Molecular studies on interspersed repetitive and unique sequences in the region of the complementation group uncoordinated on the X chromosome of Drosophila melanogaster.