| Literature DB >> 1103146 |
J R Cameron, S M Panasenko, I R Lehman, R W Davis.
Abstract
DNA from lambdagt-lambdaB bacteriophage was cleaved with EcoRI endonuclease and fragments from EcoRI-digested E. coli DNA were inserted. This DNA was used to infect E. coli, and phages containing the gene for DNA ligase were isolated by genetic selection. Two different hybrids were found with the same E. coli segment inserted in opposite orientations. Both hybrids produced similar levels of ligase as measured in crude extracts of infected cells.Entities:
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Year: 1975 PMID: 1103146 PMCID: PMC433004 DOI: 10.1073/pnas.72.9.3416
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN: 0027-8424 Impact factor: 11.205