Literature DB >> 8186414

Genomic cloning and partial characterization of human chymotrypsinogen gene.

D X Hou1, K Ozawa, N Tomita, Y Maeda, T Hashiguchi, K Yokoyama, E Soeda.   

Abstract

Chymotrypsinogen is a principal precursor of pancreatic proteolytic enzymes. We previously isolated a cDNA clone for human prechymotrypsinogen from a human pancreatic cDNA library. In the present study, we used this cDNA sequences to isolate genomic DNA clones. Three overlapping cosmid clones spanning approximately 65-kb genomic sequences were isolated from a human cosmid library. The genomic DNA clones were characterized by restriction enzyme mapping and by hybridizing them to subfragments of the cDNA. The sequence tagged sites for human chymotrypsinogen gene were created by designing two oligonucleotides. Furthermore, the isolated genomic clones were confirmed to be localized on chromosome 16q23 by fluorescence in situ hybridization and G-banding analysis.

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Year:  1993        PMID： 8186414     DOI： 10.1007/BF01907983

Source DB:  PubMed          Journal:  Jpn J Hum Genet        ISSN： 0916-8478


  9 in total

1.  The two human chymotrypsinogens. Purification and characterization.

Authors:  A De Caro; C Figarella; O Guy
Journal:  Biochim Biophys Acta       Date:  1975-02-27

2.  High-resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones.

Authors:  P Lichter; C J Tang; K Call; G Hermanson; G A Evans; D Housman; D C Ward
Journal:  Science       Date:  1990-01-05       Impact factor: 47.728

3.  Analysis of gene structure and antigen determinants of DR2 antigens using DR gene transfer into mouse L cells.

Authors:  J Kawai; A Ando; T Sato; T Nakatsuji; K Tsuji; H Inoko
Journal:  J Immunol       Date:  1989-01-01       Impact factor: 5.422

4.  Molecular cloning and nucleotide sequence of human pancreatic prechymotrypsinogen cDNA.

Authors:  N Tomita; Y Izumoto; A Horii; S Doi; H Yokouchi; M Ogawa; T Mori; K Matsubara
Journal:  Biochem Biophys Res Commun       Date:  1989-01-31       Impact factor: 3.575

5.  Isolation and sequence of a rat chymotrypsin B gene.

Authors:  G I Bell; C Quinto; M Quiroga; P Valenzuela; C S Craik; W J Rutter
Journal:  J Biol Chem       Date:  1984-11-25       Impact factor: 5.157

6.  A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

Authors:  A P Feinberg; B Vogelstein
Journal:  Anal Biochem       Date:  1983-07-01       Impact factor: 3.365

7.  Chromosomal assignments of human genes for serine proteases trypsin, chymotrypsin B, and elastase.

Authors:  N K Honey; A Y Sakaguchi; C Quinto; R J MacDonald; G I Bell; C Craik; W J Rutter; S L Naylor
Journal:  Somat Cell Mol Genet       Date:  1984-07

8.  High-resolution chromosomal localization of human genes for amylase, proopiomelanocortin, somatostatin, and a DNA fragment (D3S1) by in situ hybridization.

Authors:  B U Zabel; S L Naylor; A Y Sakaguchi; G I Bell; T B Shows
Journal:  Proc Natl Acad Sci U S A       Date:  1983-11       Impact factor: 11.205

9.  Tyrosine aminotransferase and chymotrypsinogen B are linked to haptoglobin on human chromosome 16q: comparison of genetic and physical distances.

Authors:  E M Westphal; M Burmeister; T F Wienker; H Lehrach; K Bender; G Scherer
Journal:  Genomics       Date:  1987-12       Impact factor: 5.736
  9 in total
  1 in total

1.  Detection of the pancreas-specific gene in the peripheral blood of patients with pancreatic carcinoma.

Authors:  T Kuroki; T Tomioka; Y Tajima; K Inoue; Y Ikematsu; K Ichinose; J Furui; T Kanematsu
Journal:  Br J Cancer       Date:  1999-09       Impact factor: 7.640
  1 in total

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