Literature DB >> 6196780

High-resolution chromosomal localization of human genes for amylase, proopiomelanocortin, somatostatin, and a DNA fragment (D3S1) by in situ hybridization.

B U Zabel, S L Naylor, A Y Sakaguchi, G I Bell, T B Shows.   

Abstract

The method of in situ hybridization has become a significant technique for specific-site chromosome mapping. We show that the resolution of in situ hybridization can be increased by hybridizing the probe to stretched prometaphase chromosomes with high-resolution banding obtained after 5-bromodeoxyuridine treatment of the cells and with a Hoechst 33258/Giemsa chromosome-staining method. Using this procedure, we assigned to specific chromosome sites three cloned genes and one DNA polymorphism: amylase gene (AMY) to 1p21; proopiomelanocortin gene (POMC) to 2p23, somatostatin gene (SST) to 3q28, and a single copy DNA segment (D3S1) to 3q12.

Entities:  

Mesh：

Substances：

Year:  1983        PMID： 6196780      PMCID： PMC390100          DOI： 10.1073/pnas.80.22.6932

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  18 in total

1.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

2.  Chromosomal localization of a single copy gene by in situ hybridization--human beta globin genes on the short arm of chromosome 11.

Authors:  S Malcolm; P Barton; C Murphy; M A Ferguson-Smith
Journal:  Ann Hum Genet       Date:  1981-05       Impact factor: 1.670

3.  Human immunoglobulin heavy chain genes map to a region of translocations in malignant B lymphocytes.

Authors:  I R Kirsch; C C Morton; K Nakahara; P Leder
Journal:  Science       Date:  1982-04-16       Impact factor: 47.728

4.  Localization of the human insulin gene to the distal end of the short arm of chromosome 11.

Authors:  M E Harper; A Ullrich; G F Saunders
Journal:  Proc Natl Acad Sci U S A       Date:  1981-07       Impact factor: 11.205

5.  Localization of a unique gene by direct hybridization in situ.

Authors:  D S Gerhard; E S Kawasaki; F C Bancroft; P Szabo
Journal:  Proc Natl Acad Sci U S A       Date:  1981-06       Impact factor: 11.205

6.  Differential Giemsa staining of sister chromatids and the study of chromatid exchanges without autoradiography.

Authors:  S Wolff; P Perry
Journal:  Chromosoma       Date:  1974       Impact factor: 4.316

7.  Localization of single copy DNA sequences of G-banded human chromosomes by in situ hybridization.

Authors:  M E Harper; G F Saunders
Journal:  Chromosoma       Date:  1981       Impact factor: 4.316

8.  Simple differential Giemsa staining of sister chromatids after treatment with photosensitive dyes and exposure to light and the mechanism of staining.

Authors:  K Goto; T Akematsu; H Shimazu; T Sugiyama
Journal:  Chromosoma       Date:  1975-12-10       Impact factor: 4.316

9.  High resolution R- and G-banding on the same preparation.

Authors:  B Dutrillaux; E Viegas-Pequignot
Journal:  Hum Genet       Date:  1981       Impact factor: 4.132

10.  The proopiocortin (adrenocorticotropin/beta-lipoprotein) gene is located on chromosome 2 in humans.

Authors:  D Owerbach; W J Rutter; J L Roberts; P Whitfeld; J Shine; P H Seeburg; T B Shows
Journal:  Somatic Cell Genet       Date:  1981-05
View more
  86 in total

1.  Variation in gene copy number and polymorphism of the human salivary amylase isoenzyme system in Caucasians.

Authors:  R A Bank; E H Hettema; M A Muijs; G Pals; F Arwert; D I Boomsma; J C Pronk
Journal:  Hum Genet       Date:  1992-05       Impact factor: 4.132

Review 2.  Comparative map for mice and humans.

Authors:  J H Nadeau; M T Davisson; D P Doolittle; P Grant; A L Hillyard; M R Kosowsky; T H Roderick
Journal:  Mamm Genome       Date:  1992       Impact factor: 2.957

Review 3.  Mouse chromosome 12.

Authors:  P D'Eustachio
Journal:  Mamm Genome       Date:  1992       Impact factor: 2.957

4.  Mapping small DNA sequences by fluorescence in situ hybridization directly on banded metaphase chromosomes.

Authors:  Y S Fan; L M Davis; T B Shows
Journal:  Proc Natl Acad Sci U S A       Date:  1990-08       Impact factor: 11.205

5.  Assignment of human tracheobronchial mucin gene(s) to 11p15 and a tracheobronchial mucin-related sequence to chromosome 13.

Authors:  V C Nguyen; J P Aubert; M S Gross; N Porchet; P Degand; J Frézal
Journal:  Hum Genet       Date:  1990-12       Impact factor: 4.132

6.  Array-CGH and quantitative PCR genetic analysis in a case with bilateral hypoplasia of pulmonary arteries and lungs and simultaneous unilateral renal agenesis.

Authors:  Kais Hussein; Doris Steinemann; Henrike Scholz; Ralf Menkhaus; Henning Feist; Hans Kreipe
Journal:  Int J Clin Exp Pathol       Date:  2010-08-18

7.  Mapping of the mouse anonymous DNA fragment, D16Ros2, to human chromosome 3.

Authors:  A K Ratty; R Eddy; T B Shows; K W Gross
Journal:  Mamm Genome       Date:  1992       Impact factor: 2.957

8.  Localization of the pig gene ESD to chromosome 13 by in situ hybridization.

Authors:  A Graphodatsky; V Filippov; L Biltueva; N Astachova; E Violetta; T Lushnikova; A O Ruvinsky
Journal:  Mamm Genome       Date:  1992       Impact factor: 2.957

9.  Localization of the cell surface antigen CD7 by chromosomal in situ hybridization.

Authors:  E Baker; M S Sandrin; O M Garson; G R Sutherland; I F McKenzie; L M Webber
Journal:  Immunogenetics       Date:  1990       Impact factor: 2.846

10.  Localisation of the gene coding for the haemopoietic stem cell antigen CD34 to chromosome 1q32.

Authors:  S M Howell; H V Molgaard; M F Greaves; N K Spurr
Journal:  Hum Genet       Date:  1991-09       Impact factor: 4.132
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.