Literature DB >> 8169232

Overexpression of genes of an extreme thermophile Thermus thermophilus, in Escherichia coli cells.

M Ishida1, T Oshima.   

Abstract

The 3-isopropylmalate dehydrogenase gene from an extreme thermophile, Thermus thermophilus, was not expressed in Escherichia coli unless a palindromic structure around the ribosome binding site was eliminated or a leader open reading frame was introduced into the upstream flanking region of the gene. This report suggests a way to increase the expression of this gene, with a high G+C content, in E. coli.

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Year:  1994        PMID: 8169232      PMCID: PMC205423          DOI: 10.1128/jb.176.9.2767-2770.1994

Source DB:  PubMed          Journal:  J Bacteriol        ISSN: 0021-9193            Impact factor:   3.490


  32 in total

1.  Characterization of an operon encoding succinyl-CoA synthetase and malate dehydrogenase from Thermus flavus AT-62 and its expression in Escherichia coli.

Authors:  M Nishiyama; S Horinouchi; T Beppu
Journal:  Mol Gen Genet       Date:  1991-04

2.  Molecular cloning of phosphofructokinase 1 gene from a thermophilic bacterium, Thermus thermophilus.

Authors:  J Xu; M Seki; K Denda; M Yoshida
Journal:  Biochem Biophys Res Commun       Date:  1991-05-15       Impact factor: 3.575

3.  Molecular cloning and nucleotide sequence of the gene encoding a H2O2-forming NADH oxidase from the extreme thermophilic Thermus thermophilus HB8 and its expression in Escherichia coli.

Authors:  H J Park; R Kreutzer; C O Reiser; M Sprinzl
Journal:  Eur J Biochem       Date:  1992-05-01

4.  Overexpression of the Thermus aquaticus B malate dehydrogenase-encoding gene in Escherichia coli.

Authors:  R M Alldread; D J Nicholls; T K Sundaram; M D Scawen; T Atkinson
Journal:  Gene       Date:  1992-05-01       Impact factor: 3.688

5.  Transformation and preservation of competent bacterial cells by freezing.

Authors:  D A Morrison
Journal:  Methods Enzymol       Date:  1979       Impact factor: 1.600

6.  Glutamyl-tRNA synthetase from Thermus thermophilus HB8. Molecular cloning of the gltX gene and crystallization of the overproduced protein.

Authors:  O Nureki; K Suzuki; M Hara-Yokoyama; T Kohno; H Matsuzawa; T Ohta; T Shimizu; K Morikawa; T Miyazawa; S Yokoyama
Journal:  Eur J Biochem       Date:  1992-03-01

7.  Molecular cloning of the isocitrate dehydrogenase gene of an extreme thermophile, Thermus thermophilus HB8.

Authors:  K Miyazaki; H Eguchi; A Yamagishi; T Wakagi; T Oshima
Journal:  Appl Environ Microbiol       Date:  1992-01       Impact factor: 4.792

8.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

9.  Expression, purification, and characterization of a recombinant ribonuclease H from Thermus thermophilus HB8.

Authors:  S Kanaya; M Itaya
Journal:  J Biol Chem       Date:  1992-05-15       Impact factor: 5.157

10.  Cloning and expression of the leucine gene from Thermus thermophilus in Escherichia coli.

Authors:  K Nagahari; T Koshikawa; K Sakaguchi
Journal:  Gene       Date:  1980-07       Impact factor: 3.688

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  5 in total

1.  Cloning of the gene encoding a novel thermostable alpha-galactosidase from Thermus brockianus ITI360.

Authors:  O Fridjonsson; H Watzlawick; A Gehweiler; T Rohrhirsch; R Mattes
Journal:  Appl Environ Microbiol       Date:  1999-09       Impact factor: 4.792

2.  Heterologous gene expression in Thermus thermophilus: beta-galactosidase, dibenzothiophene monooxygenase, PNB carboxy esterase, 2-aminobiphenyl-2,3-diol dioxygenase, and chloramphenicol acetyl transferase.

Authors:  Ho-Shin Park; Kevin J Kayser; Jung-Ho Kwak; John J Kilbane
Journal:  J Ind Microbiol Biotechnol       Date:  2004-05-12       Impact factor: 3.346

3.  A novel bicistronic vector for overexpressing Mycobacterium tuberculosis proteins in Escherichia coli.

Authors:  Yin Guo; Susan S Wallace; Viswanath Bandaru
Journal:  Protein Expr Purif       Date:  2008-12-30       Impact factor: 1.650

4.  Molecular cloning of the pyrE gene from the extreme thermophile Thermus flavus.

Authors:  V Vonstein; S P Johnson; H Yu; M J Casadaban; N C Pagratis; J M Weber; D C Demirjian
Journal:  J Bacteriol       Date:  1995-08       Impact factor: 3.490

5.  First Glycoside Hydrolase Family 2 Enzymes from Thermus antranikianii and Thermus brockianus with β-Glucosidase Activity.

Authors:  Carola Schröder; Saskia Blank; Garabed Antranikian
Journal:  Front Bioeng Biotechnol       Date:  2015-06-03
  5 in total

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