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Literature DB >> 7635839

Molecular cloning of the pyrE gene from the extreme thermophile Thermus flavus.

V Vonstein¹, S P Johnson, H Yu, M J Casadaban, N C Pagratis, J M Weber, D C Demirjian.

Abstract

Mutants of the extreme thermophile Thermus flavus in the pyrimidine biosynthetic pathway (Pyr-) were isolated by resistance to 5-fluoroorotic acid. The pyrE gene, which codes for the orotate phosphoribosyltransferase, was cloned by recombination with one of the isolated Pyr- T. flavus mutant strains. It was subcloned by complementation of an Escherichia coli pyrE mutant strain and was sequenced. The deduced polypeptide sequence extends over 183 amino acids. Several independent Pyr- mutations were mapped within the pyrE locus by recombination with fragments of the cloned gene.

Entities: Chemical Species

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Year: 1995 PMID： 7635839 PMCID： PMC177211 DOI： 10.1128/jb.177.15.4540-4543.1995

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

27 in total

1. Purification and characterization of a repressible alkaline phosphatase from Thermus aquaticus.

Authors: M F Yeh; J M Trela
Journal: J Biol Chem Date: 1976-05-25 Impact factor: 5.157

2. Characterization of an operon encoding succinyl-CoA synthetase and malate dehydrogenase from Thermus flavus AT-62 and its expression in Escherichia coli.

Authors: M Nishiyama; S Horinouchi; T Beppu
Journal: Mol Gen Genet Date: 1991-04

3. Screening for thermostable mutant of kanamycin nucleotidyltransferase by the use of a transformation system for a thermophile, Bacillus stearothermophilus.

Authors: M Matsumura; S Aiba
Journal: J Biol Chem Date: 1985-12-05 Impact factor: 5.157

4. Beta-galactosidase gene fusions for analyzing gene expression in escherichia coli and yeast.

Authors: M J Casadaban; A Martinez-Arias; S K Shapira; J Chou
Journal: Methods Enzymol Date: 1983 Impact factor: 1.600

5. Isolation of a thermostable enzyme variant by cloning and selection in a thermophile.

Authors: H Liao; T McKenzie; R Hageman
Journal: Proc Natl Acad Sci U S A Date: 1986-02 Impact factor: 11.205

6. Xylose (glucose) isomerase gene from the thermophile Thermus thermophilus: cloning, sequencing, and comparison with other thermostable xylose isomerases.

Authors: K Dekker; H Yamagata; K Sakaguchi; S Udaka
Journal: J Bacteriol Date: 1991-05 Impact factor: 3.490

Molecular cloning of the pyrE gene from the extreme thermophile Thermus flavus.

1. Purification and characterization of a repressible alkaline phosphatase from Thermus aquaticus.

2. Characterization of an operon encoding succinyl-CoA synthetase and malate dehydrogenase from Thermus flavus AT-62 and its expression in Escherichia coli.

3. Screening for thermostable mutant of kanamycin nucleotidyltransferase by the use of a transformation system for a thermophile, Bacillus stearothermophilus.

4. Beta-galactosidase gene fusions for analyzing gene expression in escherichia coli and yeast.

5. Isolation of a thermostable enzyme variant by cloning and selection in a thermophile.

6. Xylose (glucose) isomerase gene from the thermophile Thermus thermophilus: cloning, sequencing, and comparison with other thermostable xylose isomerases.

7. A plasmid vector for an extreme thermophile, Thermus thermophilus.

8. DNA sequencing with chain-terminating inhibitors.

9. Genetic transformation of the extreme thermophile Thermus thermophilus and of other Thermus spp.

10. A chromosome integration system for stable gene transfer into Thermus flavus.

1. Remembering Malcolm J. Casadaban.

2. Structure and expression of a pyrimidine gene cluster from the extreme thermophile Thermus strain ZO5.

3. Pyrimidine biosynthesis genes (pyrE and pyrF) of an extreme thermophile, Thermus thermophilus.

4. Orotate phosphoribosyltransferase from Corynebacterium ammoniagenes lacking a conserved lysine.