Literature DB >> 8135524

Recognition of chimeric small-subunit ribosomal DNAs composed of genes from uncultivated microorganisms.

E D Kopczynski1, M M Bateson, D M Ward.   

Abstract

When PCR was used to recover small-subunit (SSU) rRNA genes from a hot spring cyanobacterial mat community, chimeric SSU rRNA sequences which exhibited little or no secondary structural abnormality were recovered. They were revealed as chimeras of SSU rRNA genes of uncultivated species through separate phylogenetic analysis of short sequence domains.

Keywords:  NASA Discipline Exobiology; Non-NASA Center

Mesh:

Substances:

Year:  1994        PMID: 8135524      PMCID: PMC201378          DOI: 10.1128/aem.60.2.746-748.1994

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN: 0099-2240            Impact factor:   4.792


  14 in total

1.  Novel major archaebacterial group from marine plankton.

Authors:  J A Fuhrman; K McCallum; A A Davis
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2.  Chimeric cDNA clones: a novel PCR artifact.

Authors:  R H Brakenhoff; J G Schoenmakers; N H Lubsen
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3.  Occurrence of novel groups of the domain Bacteria as revealed by analysis of genetic material isolated from an Australian terrestrial environment.

Authors:  W Liesack; E Stackebrandt
Journal:  J Bacteriol       Date:  1992-08       Impact factor: 3.490

4.  16S rRNA sequences reveal numerous uncultured microorganisms in a natural community.

Authors:  D M Ward; R Weller; M M Bateson
Journal:  Nature       Date:  1990-05-03       Impact factor: 49.962

5.  DNA damage promotes jumping between templates during enzymatic amplification.

Authors:  S Pääbo; D M Irwin; A C Wilson
Journal:  J Biol Chem       Date:  1990-03-15       Impact factor: 5.157

6.  The Ribosomal Database Project.

Authors:  G J Olsen; R Overbeek; N Larsen; T L Marsh; M J McCaughey; M A Maciukenas; W M Kuan; T J Macke; Y Xing; C R Woese
Journal:  Nucleic Acids Res       Date:  1992-05-11       Impact factor: 16.971

7.  DNA recombination during PCR.

Authors:  A Meyerhans; J P Vartanian; S Wain-Hobson
Journal:  Nucleic Acids Res       Date:  1990-04-11       Impact factor: 16.971

8.  Hybrid DNA artifact from PCR of closely related target sequences.

Authors:  A R Shuldiner; A Nirula; J Roth
Journal:  Nucleic Acids Res       Date:  1989-06-12       Impact factor: 16.971

9.  The ribosomal database project.

Authors:  N Larsen; G J Olsen; B L Maidak; M J McCaughey; R Overbeek; T J Macke; T L Marsh; C R Woese
Journal:  Nucleic Acids Res       Date:  1993-07-01       Impact factor: 16.971

10.  Genetic diversity in Sargasso Sea bacterioplankton.

Authors:  S J Giovannoni; T B Britschgi; C L Moyer; K G Field
Journal:  Nature       Date:  1990-05-03       Impact factor: 49.962

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  66 in total

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Authors:  U Nübel; M M Bateson; M T Madigan; M Kühl; D M Ward
Journal:  Appl Environ Microbiol       Date:  2001-09       Impact factor: 4.792

5.  Enrichment culture and microscopy conceal diverse thermophilic Synechococcus populations in a single hot spring microbial mat habitat.

Authors:  M J Ferris; A L Ruff-Roberts; E D Kopczynski; M M Bateson; D M Ward
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9.  Succession of bacterial community structure along the Changjiang River determined by denaturing gradient gel electrophoresis and clone library analysis.

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10.  A 2-year assessment of the main environmental factors driving the free-living bacterial community structure in Lake Bourget (France).

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