Literature DB >> 8117092

Use of solid-phase PCR for enhanced detection of airborne microorganisms.

A J Alvarez1, M P Buttner, G A Toranzos, E A Dvorsky, A Toro, T B Heikes, L E Mertikas-Pifer, L D Stetzenbach.   

Abstract

The solid-phase PCR (SP-PCR) was compared with a culture-based technique for the detection of aerosolized Escherichia coli DH1. Results with SP-PCR showed an increase in detection sensitivity over that of culture methods. Therefore, SP-PCR may be useful for the detection of airborne microorganisms which may be nonculturable because of aerosolization or sampling stress.

Entities:  

Mesh：

Substances：

Year:  1994        PMID： 8117092      PMCID： PMC201317          DOI： 10.1128/aem.60.1.374-376.1994

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  8 in total

1.  Comparison of bioaerosol sampling methods in barns housing swine.

Authors:  P S Thorne; M S Kiekhaefer; P Whitten; K J Donham
Journal:  Appl Environ Microbiol       Date:  1992-08       Impact factor: 4.792

2.  Evaluation of Four Aerobiological Sampling Methods for the Retrieval of Aerosolized Pseudomonas syringae.

Authors:  M P Buttner; L D Stetzenbach
Journal:  Appl Environ Microbiol       Date:  1991-04       Impact factor: 4.792

3.  Aerial Dispersal and Epiphytic Survival of Pseudomonas syringae during a Pretest for the Release of Genetically Engineered Strains into the Environment.

Authors:  S E Lindow; G R Knudsen; R J Seidler; M V Walter; V W Lambou; P S Amy; D Schmedding; V Prince; S Hern
Journal:  Appl Environ Microbiol       Date:  1988-06       Impact factor: 4.792

4.  Solid-phase polymerase chain reaction: applications for direct detection of enteric pathogens in waters.

Authors:  G A Toranzos; A J Alvarez
Journal:  Can J Microbiol       Date:  1992-05       Impact factor: 2.419

5.  Membrane bound PCR.

Authors:  Y Kadokami; R V Lewis
Journal:  Nucleic Acids Res       Date:  1990-05-25       Impact factor: 16.971

6.  Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

Authors:  R K Saiki; D H Gelfand; S Stoffel; S J Scharf; R Higuchi; G T Horn; K B Mullis; H A Erlich
Journal:  Science       Date:  1988-01-29       Impact factor: 47.728

7.  Precision of the all-glass impinger and the andersen microbial impactor for air sampling in solid-waste handling facilities.

Authors:  L L Lembke; R N Kniseley; R C van Nostrand; M D Hale
Journal:  Appl Environ Microbiol       Date:  1981-08       Impact factor: 4.792

8.  Polymerase chain reaction detection of nonviable bacterial pathogens.

Authors:  K L Josephson; C P Gerba; I L Pepper
Journal:  Appl Environ Microbiol       Date:  1993-10       Impact factor: 4.792
  8 in total
  12 in total

1.  Enhanced detection of surface-associated bacteria in indoor environments by quantitative PCR.

Authors:  M P Buttner; P Cruz-Perez; L D Stetzenbach
Journal:  Appl Environ Microbiol       Date:  2001-06       Impact factor: 4.792

2.  Microbial structure and chemical components of aerosols caused by rotating brushes in a wastewater treatment plant.

Authors:  Yunping Han; Lin Li; Junxin Liu; Mengzhu Zhang
Journal:  Environ Sci Pollut Res Int       Date:  2012-04-17       Impact factor: 4.223

Review 3.  Inhibition and facilitation of nucleic acid amplification.

Authors:  I G Wilson
Journal:  Appl Environ Microbiol       Date:  1997-10       Impact factor: 4.792

4.  Effect of aerosolization on culturability and viability of gram-negative bacteria.

Authors:  J F Heidelberg; M Shahamat; M Levin; I Rahman; G Stelma; C Grim; R R Colwell
Journal:  Appl Environ Microbiol       Date:  1997-09       Impact factor: 4.792

5.  Improved method of detection and molecular typing of Borrelia burgdorferi sensu lato in clinical samples by polymerase chain reaction without DNA purification.

Authors:  N Rudenko; M Golovchenko; J Nemec; J Volkaert; N Mallátová; L Grubhoffer
Journal:  Folia Microbiol (Praha)       Date:  2005       Impact factor: 2.099

6.  Methods for integrated air sampling and dna analysis for detection of airborne fungal spores.

Authors:  R H Williams; E Ward; H A McCartney
Journal:  Appl Environ Microbiol       Date:  2001-06       Impact factor: 4.792

Review 7.  Paleomicrobiology: a Snapshot of Ancient Microbes and Approaches to Forensic Microbiology.

Authors:  Jessica I Rivera-Perez; Tasha M Santiago-Rodriguez; Gary A Toranzos
Journal:  Microbiol Spectr       Date:  2016-08

8.  PCR for bioaerosol monitoring: sensitivity and environmental interference.

Authors:  A J Alvarez; M P Buttner; L D Stetzenbach
Journal:  Appl Environ Microbiol       Date:  1995-10       Impact factor: 4.792

Review 9.  Review of bioaerosols in indoor environment with special reference to sampling, analysis and control mechanisms.

Authors:  Bipasha Ghosh; Himanshu Lal; Arun Srivastava
Journal:  Environ Int       Date:  2015-10-03       Impact factor: 9.621

10.  Development of an automated wet-cyclone system for rapid, continuous and enriched bioaerosol sampling and its application to real-time detection.

Authors:  Yu Sung Cho; Seung Chan Hong; Jeongan Choi; Jae Hee Jung
Journal:  Sens Actuators B Chem       Date:  2018-12-30       Impact factor: 7.460
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.