Literature DB >> 808702

The comparison of rat liver rough endoplasmic reticulum membrane proteins before and after in vitro removal of its bound ribosomes.

H H Czosnek, N de Groot, A A Hochberg.   

Abstract

The comparison of the proteins of rat liver rough membrane after stripping with EDTA or KCl-puromycin by two dimensional gel electrophoresis is described. By stripping the membrane with EDTA, most of the basic ribosomal proteins are still attached to the membrane; in contrast to the EDTA stripping method, treatment with KCl-puromycin removes most of the ribosomal proteins and does not remove any of the membranal proteins.

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Year:  1975        PMID： 808702     DOI： 10.1007/BF00357541

Source DB:  PubMed          Journal:  Mol Biol Rep        ISSN： 0301-4851            Impact factor:   2.316


  12 in total

1.  The binding of polysomes to smooth membranes of rat liver promoted by steroid hormones and extracts from either rough endoplasmic reticulum or from polysomes of the opposite sex.

Authors:  A Roobol; B R. Rabin
Journal:  FEBS Lett       Date:  1971-04-30       Impact factor: 4.124

2.  The separation of rat liver endoplasmic reticulum membrane proteins by two dimensional polyacrylamide gel electrophoresis.

Authors:  H H Czosnek; A A Hochberg
Journal:  Mol Biol Rep       Date:  1975-03       Impact factor: 2.316

3.  A comparative study of the molecular structures of the plasma membranes and the smooth and the rough endoplasmic-reticulum membranes from rat liver.

Authors:  F Morin; S Tay; H Simpkins
Journal:  Biochem J       Date:  1972-09       Impact factor: 3.857

4.  Ribosomal-membrane interaction: in vitro binding of ribosomes to microsomal membranes.

Authors:  N Borgese; W Mok; G Kreibich; D D Sabatini
Journal:  J Mol Biol       Date:  1974-09-25       Impact factor: 5.469

5.  Preferential synthesis of ferritin and albumin by different populations of liver polysomes.

Authors:  S J Hicks; J W Drysdale; H N Munro
Journal:  Science       Date:  1969-05-02       Impact factor: 47.728

6.  Biosynthesis of serum proteins and ferritin by free and attached ribosomes of rat liver.

Authors:  C M Redman
Journal:  J Biol Chem       Date:  1969-08-25       Impact factor: 5.157

7.  Binding of rat liver and hepatoma polyribosomes to stripped rough endoplasmic reticulum in vitro. Biological or an artifact?

Authors:  A A Hochberg; F W Stratman; R N Zahlten; H P Morris; H A Lardy
Journal:  Biochem J       Date:  1972-11       Impact factor: 3.857

8.  An improved cell fractionation procedure for the preparation of rat liver membrane-bound ribosomes.

Authors:  M R Adelman; G Blobel; D D Sabatini
Journal:  J Cell Biol       Date:  1973-01       Impact factor: 10.539

9.  Isolation and properties of polyribosomes and fragments of the endoplasmic reticulum from rat liver.

Authors:  H Bloemendal; W S Bont; M de Vries; E L Benedetti
Journal:  Biochem J       Date:  1967-04       Impact factor: 3.857

10.  Ribosome-membrane interaction. Nondestructive disassembly of rat liver rough microsomes into ribosomal and membranous components.

Authors:  M R Adelman; D D Sabatini; G Blobel
Journal:  J Cell Biol       Date:  1973-01       Impact factor: 10.539
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  1 in total

1.  Studies on the amino acid-incorporating activity of native rat liver rough membrane and that reconstituted in vitro.

Authors:  N De Groot; I J Yuli; H Czosnek; Y Shiklosh; A A Hochberg
Journal:  Biochem J       Date:  1976-07-15       Impact factor: 3.857
  1 in total

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