Literature DB >> 4345164

An improved cell fractionation procedure for the preparation of rat liver membrane-bound ribosomes.

M R Adelman, G Blobel, D D Sabatini.   

Abstract

A cell fractionation procedure is described which allows the preparation from rat liver of a rough microsome population containing almost 50% of the membrane-bound ribosomes of the tissue. The fraction is not contaminated with free ribosomes or smooth microsomes, and, by various other criteria, is suitable for studies of ribosome-membrane interaction.

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Year:  1973        PMID： 4345164      PMCID： PMC2108827          DOI： 10.1083/jcb.56.1.191

Source DB:  PubMed          Journal:  J Cell Biol        ISSN： 0021-9525            Impact factor:   10.539


  22 in total

1.  A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.

Authors:  K BURTON
Journal:  Biochem J       Date:  1956-02       Impact factor: 3.857

2.  Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue.

Authors:  C DE DUVE; B C PRESSMAN; R GIANETTO; R WATTIAUX; F APPELMANS
Journal:  Biochem J       Date:  1955-08       Impact factor: 3.857

3.  Isolation of rough and smooth microsomes from rat liver by means of a commerically available centrifuge.

Authors:  A Bergstrand; G Dallner
Journal:  Anal Biochem       Date:  1969-06       Impact factor: 3.365

Review 4.  Subfractionation and composition of microsomal membranes: a review.

Authors:  G Dallner; L Ernster
Journal:  J Histochem Cytochem       Date:  1968-10       Impact factor: 2.479

5.  Agranular membranes in free polysome preparations and their possible interference in studies of protein biosynthesis.

Authors:  C N Murty; T Hallinan
Journal:  Biochem J       Date:  1969-04       Impact factor: 3.857

6.  Studies on free and membrane-bound ribosomes in rat liver. I. Distribution as related to total cellular RNA.

Authors:  G Blobel; V R Potter
Journal:  J Mol Biol       Date:  1967-06-14       Impact factor: 5.469

7.  Studies on free and membrane-bound ribosomes in rat liver. II. Interaction of ribosomes and membranes.

Authors:  G Blobel; V R Potter
Journal:  J Mol Biol       Date:  1967-06-14       Impact factor: 5.469

8.  Studies on the transfer of incomplete polypeptide chains across rat liver microsomal membranes in vitro.

Authors:  C M Redman
Journal:  J Biol Chem       Date:  1967-02-25       Impact factor: 5.157

9.  Electron microscopic examination of subcellular fractions. I. The preparation of representative samples from suspensions of particles.

Authors:  P Baudhuin; P Evrard; J Berthet
Journal:  J Cell Biol       Date:  1967-01       Impact factor: 10.539

10.  The large-scale separation of peroxisomes, mitochondria, and lysosomes from the livers of rats injected with triton WR-1339. Improved isolation procedures, automated analysis, biochemical and morphological properties of fractions.

Authors:  F Leighton; B Poole; H Beaufay; P Baudhuin; J W Coffey; S Fowler; C De Duve
Journal:  J Cell Biol       Date:  1968-05       Impact factor: 10.539
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  39 in total

1.  Labelling kinetics of RNA containg poly(A) in liver subcellular fractions.

Authors:  K Hemminki
Journal:  Mol Cell Biochem       Date:  1975-08-30       Impact factor: 3.396

2.  80 S ribosomes in Acetabularia major. Distribution and transportation within the cell.

Authors:  K Kloppstech; H G Schweiger
Journal:  Protoplasma       Date:  1975       Impact factor: 3.356

Review 3.  Compartmentation of the rough endoplasmic reticulum.

Authors:  I F Pryme
Journal:  Mol Cell Biochem       Date:  1986-06       Impact factor: 3.396

4.  Studies of poly(A+)-RNA in mouse hepatoma and cortisone-stimulated rat liver.

Authors:  I M Konstantinova; V A Kulichkova; V I Vorob'ev; E A Ratovitski; J D Shaposhnikov
Journal:  Mol Cell Biochem       Date:  1977-11-25       Impact factor: 3.396

5.  Assembly of viral membranes: nature of the association of vesicular stomatitis virus proteins to membranes.

Authors:  T G Morrison; C O McQuain
Journal:  J Virol       Date:  1978-04       Impact factor: 5.103

6.  The 170-kDa glucose-regulated stress protein is an endoplasmic reticulum protein that binds immunoglobulin.

Authors:  H Y Lin; P Masso-Welch; Y P Di; J W Cai; J W Shen; J R Subjeck
Journal:  Mol Biol Cell       Date:  1993-11       Impact factor: 4.138

7.  Release of poly A(+) messenger RNA from rat liver rough microsomes upon disassembly of bound polysomes.

Authors:  J Kruppa; D D Sabatini
Journal:  J Cell Biol       Date:  1977-08       Impact factor: 10.539

8.  Polyribosomes from Peas: V. An Attempt to Characterize the Total Free and Membrane-bound Polysomal Population.

Authors:  B A Larkins; E Davies
Journal:  Plant Physiol       Date:  1975-04       Impact factor: 8.340

9.  Cytochrome P450IID6 recognized by LKM1 antibody is not exposed on the surface of hepatocytes.

Authors:  A M Yamamoto; C Mura; C De Lemos-Chiarandini; R Krishnamoorthy; F Alvarez
Journal:  Clin Exp Immunol       Date:  1993-06       Impact factor: 4.330

10.  Tyrosyl protein kinases in normal rat liver: identification and partial characterization.

Authors:  T W Wong; A R Goldberg
Journal:  Proc Natl Acad Sci U S A       Date:  1983-05       Impact factor: 11.205
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