Literature DB >> 9077

Studies on the amino acid-incorporating activity of native rat liver rough membrane and that reconstituted in vitro.

N De Groot, I J Yuli, H Czosnek, Y Shiklosh, A A Hochberg.   

Abstract

The amino acid-incorporating activities of free polyribosomes, rough membranes and rough membranes reconstituted in vitro, derived from rat liver, were compared. The amino acid-incorporating activity of the two membrane fractions were very similar in their response towards changes in pH, Mg2+ concentration and temperature, but differed from the response of the amino acid-incorporating activity of free polyribosomes. Free polyribosomes irreversibly lost part of their amino acid-incorporating capacity after they had become bound to rough membrane, from which the original ribosomes had been removed. Ribonuclease activity present in the membrane fraction may be responsible for this loss.

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Year:  1976        PMID： 9077      PMCID： PMC1163932          DOI： 10.1042/bj1580023

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  15 in total

1.  The binding of polysomes to smooth membranes of rat liver promoted by steroid hormones and extracts from either rough endoplasmic reticulum or from polysomes of the opposite sex.

Authors:  A Roobol; B R. Rabin
Journal:  FEBS Lett       Date:  1971-04-30       Impact factor: 4.124

2.  Functions of polyribosomes attached to membranes of animal cells.

Authors:  P N. Campbell
Journal:  FEBS Lett       Date:  1970-03-16       Impact factor: 4.124

3.  The separation of rat liver endoplasmic reticulum membrane proteins by two dimensional polyacrylamide gel electrophoresis.

Authors:  H H Czosnek; A A Hochberg
Journal:  Mol Biol Rep       Date:  1975-03       Impact factor: 2.316

4.  The in vitro reconstitution of a functional rough membrane active in protein synthesis.

Authors:  A A Hochberg; H H Czosnek; T Shine; N De Groot
Journal:  Mol Biol Rep       Date:  1975-03       Impact factor: 2.316

5.  Structure of rough, smooth, stripped and reconstituted rough membranes derived from rat liver as visualized by the freeze fracture technique.

Authors:  A A Hochberg; H H Czosnek; Y Reichler; I Ohad; N De Groot
Journal:  Mol Biol Rep       Date:  1975-12       Impact factor: 2.316

6.  Peptidyl-tRNA. XII. Behaviour of peptidyl-tRNA-Phe from Escherichia coli on methylated albumin silicic acid column.

Authors:  E Ziv; N De Groot; Y Lapidot
Journal:  Biochim Biophys Acta       Date:  1971-01-01

7.  Ribosomal-membrane interaction: in vitro binding of ribosomes to microsomal membranes.

Authors:  N Borgese; W Mok; G Kreibich; D D Sabatini
Journal:  J Mol Biol       Date:  1974-09-25       Impact factor: 5.469

8.  The comparison of rat liver rough endoplasmic reticulum membrane proteins before and after in vitro removal of its bound ribosomes.

Authors:  H H Czosnek; N de Groot; A A Hochberg
Journal:  Mol Biol Rep       Date:  1975-07       Impact factor: 2.316

9.  Polyribosomal attachment to rat liver and hepatoma endoplasmic reticulum in vitro. A method for its study.

Authors:  W L Ragland; T K Shires; H C Pitot
Journal:  Biochem J       Date:  1971-01       Impact factor: 3.857

10.  Ribosome-membrane interaction. Nondestructive disassembly of rat liver rough microsomes into ribosomal and membranous components.

Authors:  M R Adelman; D D Sabatini; G Blobel
Journal:  J Cell Biol       Date:  1973-01       Impact factor: 10.539
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  2 in total

1.  The binding of monoribosomes, oligoribosomes and polyribosomes to reticular membranes from rat liver.

Authors:  J A Fielder; H M Dani; D Ridge; B R Rabin
Journal:  Biochem J       Date:  1978-04-15       Impact factor: 3.857

2.  Conjugation of [125I]ubiquitin to cellular proteins in permeabilized mammalian cells: comparison of mitotic and interphase cells.

Authors:  B Raboy; H A Parag; R G Kulka
Journal:  EMBO J       Date:  1986-05       Impact factor: 11.598
  2 in total

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