Literature DB >> 1128514

The separation of rat liver endoplasmic reticulum membrane proteins by two dimensional polyacrylamide gel electrophoresis.

H H Czosnek, A A Hochberg.   

Abstract

The separation of rat liver endoplasmic reticulum membrane proteins by two dimensional polyacrylamide gel electrophoresis is described. By this method, the proteins of the rough membrane ribosomes could be separated from the other rough membrane proteins. Both rough and smooth membrane fractions contain at least 30 defined membranal proteins. The electro-phoretic patterns of rough and smooth membrane proteins are clearly different.

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Year:  1975        PMID: 1128514     DOI: 10.1007/bf00357293

Source DB:  PubMed          Journal:  Mol Biol Rep        ISSN: 0301-4851            Impact factor:   2.316


  16 in total

1.  Physical characteristics of proteins of the electron transfer system and interpretation of the structure of the mitochondrion.

Authors:  R S CRIDDLE; R M BOCK; D E GREEN; H TISDALE
Journal:  Biochemistry       Date:  1962-09       Impact factor: 3.162

2.  A comparison of the proteins of rat skeletal muscle and liver ribosomes by two-dimensional polyacrylamide gel electrophoresis. Observations on the partition of proteins between ribosomal subunits and a description of two acidic proteins in the large subunit.

Authors:  C C Sherton; I G Wool
Journal:  J Biol Chem       Date:  1974-04-10       Impact factor: 5.157

3.  A method for changing the pH of gel strips in the two-dimensional gel electrophoresis of ribosomal proteins.

Authors:  S Avital; D Elson
Journal:  Anal Biochem       Date:  1974-01       Impact factor: 3.365

4.  Determination of the number of proteins in liver ribosomes and ribosomal subunits by two-dimensional polyacrylamide gel electrophoresis.

Authors:  C C Sherton; I G Wool
Journal:  J Biol Chem       Date:  1972-07-25       Impact factor: 5.157

5.  Role for steroid hormones in the interaction of ribosomes with the endoplasmic membranes of rat liver.

Authors:  G H Sunshine; D J Williams; B R Rabin
Journal:  Nat New Biol       Date:  1971-03-31

6.  Comparison of rat liver mitochondrial and microsomal membrane proteins.

Authors:  C A Schnaitman
Journal:  Proc Natl Acad Sci U S A       Date:  1969-06       Impact factor: 11.205

7.  Heterogeneity of reticulocyte ribosomes.

Authors:  E R Burka; S I Bulova
Journal:  Biochem Biophys Res Commun       Date:  1971-03-05       Impact factor: 3.575

8.  Ribosome-membrane interaction. Nondestructive disassembly of rat liver rough microsomes into ribosomal and membranous components.

Authors:  M R Adelman; D D Sabatini; G Blobel
Journal:  J Cell Biol       Date:  1973-01       Impact factor: 10.539

9.  Biogenesis of endoplasmic reticulum membranes. I. Structural and chemical differentiation in developing rat hepatocyte.

Authors:  G Dallner; P Siekevitz; G E Palade
Journal:  J Cell Biol       Date:  1966-07       Impact factor: 10.539

10.  Biogenesis of endoplasmic reticulum membranes. II. Synthesis of constitutive microsomal enzymes in developing rat hepatocyte.

Authors:  G Dallner; P Siekevitz; G E Palade
Journal:  J Cell Biol       Date:  1966-07       Impact factor: 10.539

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  4 in total

1.  Studies on the amino acid-incorporating activity of native rat liver rough membrane and that reconstituted in vitro.

Authors:  N De Groot; I J Yuli; H Czosnek; Y Shiklosh; A A Hochberg
Journal:  Biochem J       Date:  1976-07-15       Impact factor: 3.857

2.  Structure of rough, smooth, stripped and reconstituted rough membranes derived from rat liver as visualized by the freeze fracture technique.

Authors:  A A Hochberg; H H Czosnek; Y Reichler; I Ohad; N De Groot
Journal:  Mol Biol Rep       Date:  1975-12       Impact factor: 2.316

3.  The comparison of rat liver rough endoplasmic reticulum membrane proteins before and after in vitro removal of its bound ribosomes.

Authors:  H H Czosnek; N de Groot; A A Hochberg
Journal:  Mol Biol Rep       Date:  1975-07       Impact factor: 2.316

4.  Effect of ethionine on the rough endoplasmic reticulum from male and female rat liver.

Authors:  H H Czosnek; A Ascarelli; N De Groot; M Hergenhahn; A A Hochberg
Journal:  Mol Biol Rep       Date:  1977-12       Impact factor: 2.316

  4 in total

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