Literature DB >> 8043952

Efficient identification and regional positioning of YAC and cosmid clones to human chromosome 21 by radiation fusion hybrids.

J Kumlien1, T Labella, G Zehetner, R Vatcheva, D Nizetic, H Lehrach.   

Abstract

The use of integrated mapping strategies involving bacterial, yeast, and rodent cells as hosts simplifies the construction of maps, which combine long-range order, high resolution, and easy access to the cloned DNA. Radiation-fusion hybrids offer a specially powerful long-range mapping system for human chromosomes. We describe here techniques for establishing a radiation-fusion hybrid map of Chromosome (Chr) 21q and its integration with local information on YAC and cosmid positions.

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Year:  1994        PMID： 8043952     DOI： 10.1007/bf00356556

Source DB:  PubMed          Journal:  Mamm Genome        ISSN： 0938-8990            Impact factor:   2.957


  24 in total

1.  New method for mapping genes in human chromosomes.

Authors:  S J Goss; H Harris
Journal:  Nature       Date:  1975-06-26       Impact factor: 49.962

2.  Continuum of overlapping clones spanning the entire human chromosome 21q.

Authors:  I Chumakov; P Rigault; S Guillou; P Ougen; A Billaut; G Guasconi; P Gervy; I LeGall; P Soularue; L Grinas
Journal:  Nature       Date:  1992-10-01       Impact factor: 49.962

3.  Chromosomal assignment of human YAC clones by fluorescence in situ hybridization: use of single-yeast-colony PCR and multiple labeling.

Authors:  A Baldini; M Ross; D Nizetic; R Vatcheva; E A Lindsay; H Lehrach; M Siniscalco
Journal:  Genomics       Date:  1992-09       Impact factor: 5.736

4.  An improved bacterial colony lysis procedure enables direct DNA hybridisation using short (10, 11 bases) oligonucleotides to cosmids.

Authors:  D Nizetic; R Drmanac; H Lehrach
Journal:  Nucleic Acids Res       Date:  1991-01-11       Impact factor: 16.971

5.  Mapping irradiation hybrids to cosmid and yeast artificial chromosome libraries by direct hybridization of Alu-PCR products.

Authors:  A P Monaco; V M Lam; G Zehetner; G G Lennon; C Douglas; D Nizetic; P N Goodfellow; H Lehrach
Journal:  Nucleic Acids Res       Date:  1991-06-25       Impact factor: 16.971

6.  Yeast artificial chromosome libraries containing large inserts from mouse and human DNA.

Authors:  Z Larin; A P Monaco; H Lehrach
Journal:  Proc Natl Acad Sci U S A       Date:  1991-05-15       Impact factor: 11.205

7.  Sixty-five radiation hybrids for the short arm of human chromosome 6: their value as a mapping panel and as a source for rapid isolation of new probes using repeat element-mediated PCR.

Authors:  H Y Zoghbi; A E McCall; F LeBorgne-Demarquoy
Journal:  Genomics       Date:  1991-04       Impact factor: 5.736

8.  Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors.

Authors:  D T Burke; G F Carle; M V Olson
Journal:  Science       Date:  1987-05-15       Impact factor: 47.728

9.  Alu polymerase chain reaction: a method for rapid isolation of human-specific sequences from complex DNA sources.

Authors:  D L Nelson; S A Ledbetter; L Corbo; M F Victoria; R Ramírez-Solis; T D Webster; D H Ledbetter; C T Caskey
Journal:  Proc Natl Acad Sci U S A       Date:  1989-09       Impact factor: 11.205

10.  Generation of novel sequence tagged sites (STSs) from discrete chromosomal regions using Alu-PCR.

Authors:  C G Cole; P N Goodfellow; M Bobrow; D R Bentley
Journal:  Genomics       Date:  1991-07       Impact factor: 5.736
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  1 in total

1.  A radiation hybrid map spanning the entire human X chromosome integrating YACs, genes, and STS markers.

Authors:  J Kumlien; A Grigoriev; H Roest Crollius; M Ross; P N Goodfellow; H Lehrach
Journal:  Mamm Genome       Date:  1996-10       Impact factor: 2.957
  1 in total

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