Literature DB >> 2062647

Mapping irradiation hybrids to cosmid and yeast artificial chromosome libraries by direct hybridization of Alu-PCR products.

A P Monaco1, V M Lam, G Zehetner, G G Lennon, C Douglas, D Nizetic, P N Goodfellow, H Lehrach.   

Abstract

A direct hybridization protocol is described for screening cosmid and yeast artificial chromosome libraries with pools of Alu-PCR products from somatic cell or irradiation hybrids. This method eliminates purification, cloning and analysis of each individual Alu-PCR product before library screening. A series of human X chromosome irradiation hybrids were mapped by this method, using a cosmid reference library for comparisons between overlapping hybrids to identify interesting clones for further analysis.

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Year:  1991        PMID： 2062647      PMCID： PMC328328          DOI： 10.1093/nar/19.12.3315

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  13 in total

1.  Construction, arraying, and high-density screening of large insert libraries of human chromosomes X and 21: their potential use as reference libraries.

Authors:  D Nizetić; G Zehetner; A P Monaco; L Gellen; B D Young; H Lehrach
Journal:  Proc Natl Acad Sci U S A       Date:  1991-04-15       Impact factor: 11.205

2.  A deletion hot spot in the Duchenne muscular dystrophy gene.

Authors:  M C Wapenaar; T Kievits; K A Hart; S Abbs; L A Blonden; J T den Dunnen; P M Grootscholten; E Bakker; C Verellen-Dumoulin; M Bobrow
Journal:  Genomics       Date:  1988-02       Impact factor: 5.736

3.  Rapid isolation of human chromosome-specific DNA probes from a somatic cell hybrid.

Authors:  F E Cotter; G M Hampton; S Nasipuri; W F Bodmer; B D Young
Journal:  Genomics       Date:  1990-06       Impact factor: 5.736

4.  Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors.

Authors:  D T Burke; G F Carle; M V Olson
Journal:  Science       Date:  1987-05-15       Impact factor: 47.728

5.  Rapid isolation of DNA probes within specific chromosome regions by interspersed repetitive sequence polymerase chain reaction.

Authors:  S A Ledbetter; D L Nelson; S T Warren; D H Ledbetter
Journal:  Genomics       Date:  1990-03       Impact factor: 5.736

6.  Cloning the gene for an inherited human disorder--chronic granulomatous disease--on the basis of its chromosomal location.

Authors:  B Royer-Pokora; L M Kunkel; A P Monaco; S C Goff; P E Newburger; R L Baehner; F S Cole; J T Curnutte; S H Orkin
Journal:  Nature       Date:  1986 Jul 3-9       Impact factor: 49.962

7.  A method for generating hybrids containing nonselected fragments of human chromosomes.

Authors:  F Benham; K Hart; J Crolla; M Bobrow; M Francavilla; P N Goodfellow
Journal:  Genomics       Date:  1989-05       Impact factor: 5.736

8.  Construction and screening of a genomic library specific for mouse chromosome 16.

Authors:  U Hochgeschwender; J G Sutcliffe; M B Brennan
Journal:  Proc Natl Acad Sci U S A       Date:  1989-11       Impact factor: 11.205

9.  "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.

Authors:  A P Feinberg; B Vogelstein
Journal:  Anal Biochem       Date:  1984-02       Impact factor: 3.365

10.  Segregation of the Huntington disease region of human chromosome 4 in a somatic cell hybrid.

Authors:  D R Cox; C A Pritchard; E Uglum; D Casher; J Kobori; R M Myers
Journal:  Genomics       Date:  1989-04       Impact factor: 5.736
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  8 in total

1.  Multiplexed sorting of libraries on libraries: a novel method for empirical protein design by affinity-driven phage enrichment on synthetic peptide arrays.

Authors:  Claus Hultschig; Ronald Frank
Journal:  Mol Divers       Date:  2004       Impact factor: 2.943

2.  A two-dimensional YAC pooling strategy for library screening via STS and Alu-PCR methods.

Authors:  C T Amemiya; M J Alegria-Hartman; C Aslanidis; C Chen; J Nikolic; J C Gingrich; P J de Jong
Journal:  Nucleic Acids Res       Date:  1992-05-25       Impact factor: 16.971

3.  A radiation hybrid map spanning the entire human X chromosome integrating YACs, genes, and STS markers.

Authors:  J Kumlien; A Grigoriev; H Roest Crollius; M Ross; P N Goodfellow; H Lehrach
Journal:  Mamm Genome       Date:  1996-10       Impact factor: 2.957

4.  Oat-maize chromosome addition lines: a new system for mapping the maize genome.

Authors:  E V Ananiev; O Riera-Lizarazu; H W Rines; R L Phillips
Journal:  Proc Natl Acad Sci U S A       Date:  1997-04-15       Impact factor: 11.205

Review 5.  Irradiation and fusion gene transfer.

Authors:  M A Walter; P N Goodfellow
Journal:  Mol Biotechnol       Date:  1995-04       Impact factor: 2.695

6.  Characterization of microsatellites from flow-sorted porcine chromosome 13.

Authors:  W Davies; B Høyheim; B Chaput; A L Archibald; G Frelat
Journal:  Mamm Genome       Date:  1994-11       Impact factor: 2.957

7.  Efficient identification and regional positioning of YAC and cosmid clones to human chromosome 21 by radiation fusion hybrids.

Authors:  J Kumlien; T Labella; G Zehetner; R Vatcheva; D Nizetic; H Lehrach
Journal:  Mamm Genome       Date:  1994-06       Impact factor: 2.957

8.  W (A or T) sequences as probes and primers suitable for genomic mapping and fingerprinting.

Authors:  R Drmanac; D Nizetic; G G Lennon; A Beitverda; H Lehrach
Journal:  Nucleic Acids Res       Date:  1991-11-11       Impact factor: 16.971
  8 in total

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