Literature DB >> 1889821

Generation of novel sequence tagged sites (STSs) from discrete chromosomal regions using Alu-PCR.

C G Cole1, P N Goodfellow, M Bobrow, D R Bentley.   

Abstract

Human DNA segments from discrete chromosomal regions were generated by utilizing Alu-element-based polymerase chain reaction (Alu-PCR) of an irradiation-fusion hybrid containing approximately 10 to 15 Mb of human DNA. Following cloning into a plasmid vector, a subset of the clones was used to generate sequence tagged sites (STSs) de novo. By means of a panel of hybrids containing portions of the human X chromosome, the STSs were shown to localize to two chromosomal regions, Xq24-Xq26 and Xcen-Xq13, reflecting the presence in the irradiation-fusion hybrid of two human chromosome fragments. These results demonstrate that high densities of STSs can be rapidly and efficiently generated from defined regions of the human genome using Alu-PCR.

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Year:  1991        PMID： 1889821     DOI： 10.1016/0888-7543(91)90468-t

Source DB:  PubMed          Journal:  Genomics        ISSN： 0888-7543            Impact factor:   5.736


  14 in total

1.  High-resolution landmark framework for the sequence-ready mapping of Xq23-q26.1.

Authors:  H E Steingruber; A Dunham; A J Coffey; S M Clegg; G R Howell; G L Maslen; C E Scott; R Gwilliam; P J Hunt; E C Sotheran; E J Huckle; S E Hunt; P Dhami; C Soderlund; M A Leversha; D R Bentley; M T Ross
Journal:  Genome Res       Date:  1999-08       Impact factor: 9.043

2.  Artiodactyl retroposons: association with microsatellites and use in SINEmorph detection by PCR.

Authors:  J Kaukinen; S L Varvio
Journal:  Nucleic Acids Res       Date:  1992-06-25       Impact factor: 16.971

3.  A two-dimensional YAC pooling strategy for library screening via STS and Alu-PCR methods.

Authors:  C T Amemiya; M J Alegria-Hartman; C Aslanidis; C Chen; J Nikolic; J C Gingrich; P J de Jong
Journal:  Nucleic Acids Res       Date:  1992-05-25       Impact factor: 16.971

4.  An Alu polymorphism intragenic to the TP53 gene.

Authors:  P A Futreal; J C Barrett; R W Wiseman
Journal:  Nucleic Acids Res       Date:  1991-12-25       Impact factor: 16.971

5.  Sequence-tagged-site-facilitated PCR for barley genome mapping.

Authors:  S Tragoonrung; V Kanazin; P M Hayes; T K Blake
Journal:  Theor Appl Genet       Date:  1992-09       Impact factor: 5.699

6.  A radiation hybrid map spanning the entire human X chromosome integrating YACs, genes, and STS markers.

Authors:  J Kumlien; A Grigoriev; H Roest Crollius; M Ross; P N Goodfellow; H Lehrach
Journal:  Mamm Genome       Date:  1996-10       Impact factor: 2.957

7.  Androgen insensitivity with mental retardation: a contiguous gene syndrome?

Authors:  H R Davies; I A Hughes; M O Savage; C A Quigley; M Trifiro; L Pinsky; T R Brown; M N Patterson
Journal:  J Med Genet       Date:  1997-02       Impact factor: 6.318

8.  Generation of sequence-tagged sites from Xp22.3 by isolating common Alu-PCR products of radiation hybrids retaining overlapping human X chromosome fragments.

Authors:  I A Glass; M Passage; L Bernatowicz; E C Salido; T Mohandas; P H Yen; L J Shapiro
Journal:  Hum Genet       Date:  1996-05       Impact factor: 4.132

9.  Primed in situ (PRINS) labelling with Alu and satellite primers for rapid characterization of human chromosomes in hybrid cell lines.

Authors:  P Coullin; B Andréo; J P Charlieu; J J Candelier; F Pellestor
Journal:  Chromosome Res       Date:  1997-08       Impact factor: 5.239

Review 10.  Irradiation and fusion gene transfer.

Authors:  M A Walter; P N Goodfellow
Journal:  Mol Biotechnol       Date:  1995-04       Impact factor: 2.695
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