| Literature DB >> 8042908 |
P Pristas1, I Vanat, A Godany, P Javorský.
Abstract
Two natural isolates from fallow-deer rumen identified as Selenomonas ruminantium were found to produce a restriction endonuclease which we called Sru4DI. This enzyme was isolated from cell extracts by phosphocellulose chromatography. Analysis of the Sru4DI recognition site showed that Sru4DI recognizes the hexanucleotide sequence 5'-AT/TAAT-3' generating 5'dinucleotide protruding ends upon cleavage and thus is a true isoschizomer of vspI, a restriction enzyme isolated from Vibrio sp.Entities:
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Year: 1994 PMID: 8042908 DOI: 10.1007/bf00288956
Source DB: PubMed Journal: Arch Microbiol ISSN: 0302-8933 Impact factor: 2.552