Literature DB >> 3036169

[Substrate specificity of restriction endonuclease VspI].

S Kh Degtiarev, V E Repin, N I Rechkunova, V E Chizhikov, E G Malygin.   

Abstract

The recognition sequence and cleavage point of restriction endonuclease VspI have been determined as 5'-AT decreases TAAT. This enzyme is not isoschizomer of any known restriction endonucleases. DNA pBR322 contains a single VspI recognition sequence in position 3539. Therefore this enzyme may be used for cloning DNA in the VspI site in AmpR-gene of pBR322.

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Year:  1987        PMID： 3036169

Source DB:  PubMed          Journal:  Bioorg Khim        ISSN： 0132-3423


  5 in total

1.  Restriction enzymes and their isoschizomers.

Authors:  R J Roberts
Journal:  Nucleic Acids Res       Date:  1990-04-25       Impact factor: 16.971

2.  Restriction enzymes and their isoschizomers.

Authors:  R J Roberts
Journal:  Nucleic Acids Res       Date:  1989       Impact factor: 16.971

3.  AseI, a restriction endonuclease from Aquaspirillum serpens which recognizes 5'AT--TAAT3'.

Authors:  C Polisson; R D Morgan
Journal:  Nucleic Acids Res       Date:  1988-11-11       Impact factor: 16.971

4.  Restriction enzymes and their isoschizomers.

Authors:  R J Roberts
Journal:  Nucleic Acids Res       Date:  1988       Impact factor: 16.971

5.  Restriction endonucleases from Selenomonas ruminantium which recognize and cleave 5'-AT/TAAT-3'.

Authors:  P Pristas; I Vanat; A Godany; P Javorský
Journal:  Arch Microbiol       Date:  1994       Impact factor: 2.552
  5 in total

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