Literature DB >> 7764381

SbvI restriction endonuclease from Streptococcus bovis.

I Vanat1, P Pristas, E Kutejov a, J Júdová, A Godány, P Javorský.   

Abstract

Restriction endonuclease SbvI, an isoschizomer of HaeIII, has been isolated from rumen amylolytic bacterium Streptococcus bovis II/1. SbvI was purified from cell extract by phosphocellulose chromatography and heparin-Sepharose chromatography. The recognition sequence of SbvI was identified by digestion of pBR322, pUC9 and lambda-DNA and comparing the cleavage patterns obtained with computer-derived data. SbvI recognizes the 4-bp palindrome, 5'-GGCC-3' and cleaves DNA after the second G in the sequence, producing blunt ends.

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Year:  1993        PMID: 7764381     DOI: 10.1111/j.1472-765x.1993.tb01471.x

Source DB:  PubMed          Journal:  Lett Appl Microbiol        ISSN: 0266-8254            Impact factor:   2.858


  3 in total

1.  Lack of surface receptors not restriction-modification system determines F4 phage resistance in Streptococcus bovis II/1.

Authors:  I Styriak; P Pristas; P Javorský
Journal:  Folia Microbiol (Praha)       Date:  1998       Impact factor: 2.099

2.  Restriction endonucleases from Selenomonas ruminantium which recognize and cleave 5'-AT/TAAT-3'.

Authors:  P Pristas; I Vanat; A Godany; P Javorský
Journal:  Arch Microbiol       Date:  1994       Impact factor: 2.552

3.  Characterization of a complex restriction-modification system detected in Staphylococcus aureus and Streptococcus agalactiae strains isolated from infections of domestic animals.

Authors:  A Godány; G Bukovská; J Farkasovská; Z Brnáková; A Dmitriev; E Tkáciková; T Ayele; I Mikula
Journal:  Folia Microbiol (Praha)       Date:  2004       Impact factor: 2.099

  3 in total

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