Literature DB >> 995647

Isolation of native DNA fragments containing structural genes at the beginning, in the middle or at the end of the coding strand.

G N Yenikolopov, A P Ryskov, T Nitta, G P Georgiev.   

Abstract

A method for the isolation of the DNA fragments containing structural genes at the beginning, in the middle and at the end of the coding strand is described. It involves mechanical or enzymatic shearing, exonuclease treatment, hybridization with mRNA and subsequent retention of DNA-mRNA complex on poly(U)-Sepharose. Hybridization of the DNA fragments with mRNA takes place with high specificity and isolated material is enriched in structural genes. Applications of this method for gene isolation are discussed.

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Year:  1976        PMID： 995647      PMCID： PMC343119          DOI： 10.1093/nar/3.10.2645

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  14 in total

1.  Studies on nucleic acid reassociation kinetics: reactivity of single-stranded tails in DNA-DNA renaturation.

Authors:  M J Smith; R J Britten; E H Davidson
Journal:  Proc Natl Acad Sci U S A       Date:  1975-12       Impact factor: 11.205

2.  SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA.

Authors:  F W STUDIER
Journal:  J Mol Biol       Date:  1965-02       Impact factor: 5.469

3.  A new method for the isolation of deoxyribonucleic acids; evidence on the nature of bonds between deoxyribonucleic acid and protein.

Authors:  K S KIRBY
Journal:  Biochem J       Date:  1957-07       Impact factor: 3.857

4.  Direct demonstration of a complementarity between mRNA and double-stranded sequences of pre-mRNA.

Authors:  A P Ryskov; O V Tokarskaya; G P Georgiev
Journal:  Mol Biol Rep       Date:  1976-04       Impact factor: 2.316

Review 5.  Methods of gene isolation.

Authors:  D D Brown; R Stern
Journal:  Annu Rev Biochem       Date:  1974       Impact factor: 23.643

6.  A measurement of the sequence complexity of polysomal messenger RNA in sea urchin embryos.

Authors:  G A Galau; R J Britten; E H Davidson
Journal:  Cell       Date:  1974-05       Impact factor: 41.582

7.  Three abundance classes in HeLa cell messenger RNA.

Authors:  J O Bishop; J G Morton; M Rosbash; M Richardson
Journal:  Nature       Date:  1974-07-19       Impact factor: 49.962

8.  Regulation of lambda exonuclease. I. Properties of lambda exonuclease purified from lysogens of lambda T11 and wild type.

Authors:  C M Radding
Journal:  J Mol Biol       Date:  1966-07       Impact factor: 5.469

9.  Complexity of cytoplasmic RNA in different mouse tissues measured by hybridization of polyadenylated RNA to complementary DNA.

Authors:  G U Ryffel; B J McCarthy
Journal:  Biochemistry       Date:  1975-04-08       Impact factor: 3.162

10.  Cyclization of eucaryotic deoxyribonucleic acid fragments.

Authors:  C A Thomas; B A Hamkalo; D N Misra; C S Lee
Journal:  J Mol Biol       Date:  1970-08       Impact factor: 5.469
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