Literature DB >> 7972003

Isolation of the yeast histone octamer.

Y Lorch1, R D Kornberg.   

Abstract

Procedures for the extraction and purification of the yeast histone octamer are described. Either mechanical disruption, yielding chromatin fragments, or spheroplast formation with subsequent nuclear isolation was employed. A hexahistidine tag was inserted in the N-terminal region of histone H2B, permitting resolution of the histone octamer from high-salt extracts of nuclei or chromatin to near homogeneity. The histone octamer purified in this way was fully active in reconstitution of nucleosomes.

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Year:  1994        PMID： 7972003      PMCID： PMC45160          DOI： 10.1073/pnas.91.23.11032

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  27 in total

1.  Higher-order structure of Saccharomyces cerevisiae chromatin.

Authors:  P T Lowary; J Widom
Journal:  Proc Natl Acad Sci U S A       Date:  1989-11       Impact factor: 11.205

2.  Isolation and characterization of histones.

Authors:  C von Holt; W F Brandt; H J Greyling; G G Lindsey; J D Retief; J D Rodrigues; S Schwager; B T Sewell
Journal:  Methods Enzymol       Date:  1989       Impact factor: 1.600

3.  Preparation of nucleosomes and chromatin.

Authors:  R D Kornberg; J W LaPointe; Y Lorch
Journal:  Methods Enzymol       Date:  1989       Impact factor: 1.600

Review 4.  The study of histone--histone associations by chemical cross-linking.

Authors:  J O Thomas; R D Kornberg
Journal:  Methods Cell Biol       Date:  1978       Impact factor: 1.441

5.  Nucleosomes inhibit the initiation of transcription but allow chain elongation with the displacement of histones.

Authors:  Y Lorch; J W LaPointe; R D Kornberg
Journal:  Cell       Date:  1987-04-24       Impact factor: 41.582

6.  Delta sequences and double symmetry in a yeast chromosomal replicator region.

Authors:  G Tschumper; J Carbon
Journal:  J Mol Biol       Date:  1982-04-05       Impact factor: 5.469

7.  GAL4 protein: purification, association with GAL80 protein, and conserved domain structure.

Authors:  D I Chasman; R D Kornberg
Journal:  Mol Cell Biol       Date:  1990-06       Impact factor: 4.272

8.  Histone H2B repression causes cell-cycle-specific arrest in yeast: effects on chromosomal segregation, replication, and transcription.

Authors:  M Han; M Chang; U J Kim; M Grunstein
Journal:  Cell       Date:  1987-02-27       Impact factor: 41.582

9.  Five SWI/SNF gene products are components of a large multisubunit complex required for transcriptional enhancement.

Authors:  C L Peterson; A Dingwall; M P Scott
Journal:  Proc Natl Acad Sci U S A       Date:  1994-04-12       Impact factor: 11.205

10.  Yeast histone H2B containing large amino terminus deletions can function in vivo.

Authors:  J W Wallis; M Rykowski; M Grunstein
Journal:  Cell       Date:  1983-12       Impact factor: 41.582
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  5 in total

1.  General method for rapid purification of native chromatin fragments.

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Journal:  J Biol Chem       Date:  2018-05-24       Impact factor: 5.157

2.  Evidence for a mediator cycle at the initiation of transcription.

Authors:  J Q Svejstrup; Y Li; J Fellows; A Gnatt; S Bjorklund; R D Kornberg
Journal:  Proc Natl Acad Sci U S A       Date:  1997-06-10       Impact factor: 11.205

3.  Interactions of Isw2 chromatin remodeling complex with nucleosomal arrays: analyses using recombinant yeast histones and immobilized templates.

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4.  FACT, the Bur kinase pathway, and the histone co-repressor HirC have overlapping nucleosome-related roles in yeast transcription elongation.

Authors:  Jennifer R Stevens; Allyson F O'Donnell; Troy E Perry; Jeremy J R Benjamin; Christine A Barnes; Gerald C Johnston; Richard A Singer
Journal:  PLoS One       Date:  2011-10-12       Impact factor: 3.240

5.  Robust methods for purification of histones from cultured mammalian cells with the preservation of their native modifications.

Authors:  Pedro Rodriguez-Collazo; Sanford H Leuba; Jordanka Zlatanova
Journal:  Nucleic Acids Res       Date:  2009-05-13       Impact factor: 16.971
  5 in total

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