Literature DB >> 8159677

Five SWI/SNF gene products are components of a large multisubunit complex required for transcriptional enhancement.

C L Peterson1, A Dingwall, M P Scott.   

Abstract

The Saccharomyces cerevisiae SWI1, SWI2 (SNF2), SWI3, SNF5, and SNF6 gene products play a crucial role in the regulation of transcription. We provide here direct biochemical evidence that all five SWI/SNF polypeptides are components of a large multisubunit complex. These five polypeptides coelute from a gel-filtration column with an apparent molecular mass of approximately 2 MDa. The five SWI/SNF polypeptides do not copurify when extracts are prepared from swi- or snf- mutants. We show that SWI/SNF polypeptides also remain associated during an affinity-chromatography step followed by gel filtration. Assembly of the SWI/SNF complex is not disrupted by a mutation in the putative APT-binding site of SWI2, although this mutation eliminates SWI2 function.

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Year:  1994        PMID： 8159677      PMCID： PMC43482          DOI： 10.1073/pnas.91.8.2905

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  19 in total

1.  The SNF5 protein of Saccharomyces cerevisiae is a glutamine- and proline-rich transcriptional activator that affects expression of a broad spectrum of genes.

Authors:  B C Laurent; M A Treitel; M Carlson
Journal:  Mol Cell Biol       Date:  1990-11       Impact factor: 4.272

2.  Epitope tagging and protein surveillance.

Authors:  P A Kolodziej; R A Young
Journal:  Methods Enzymol       Date:  1991       Impact factor: 1.600

3.  SNF6 encodes a nuclear protein that is required for expression of many genes in Saccharomyces cerevisiae.

Authors:  F Estruch; M Carlson
Journal:  Mol Cell Biol       Date:  1990-06       Impact factor: 4.272

4.  Yeast Gal11 protein mediates the transcriptional activation signal of two different transacting factors, Gal4 and general regulatory factor I/repressor/activator site binding protein 1/translation upstream factor.

Authors:  M Nishizawa; Y Suzuki; Y Nogi; K Matsumoto; T Fukasawa
Journal:  Proc Natl Acad Sci U S A       Date:  1990-07       Impact factor: 11.205

5.  A multisubunit complex associated with the RNA polymerase II CTD and TATA-binding protein in yeast.

Authors:  C M Thompson; A J Koleske; D M Chao; R A Young
Journal:  Cell       Date:  1993-07-02       Impact factor: 41.582

6.  BRG1 contains a conserved domain of the SWI2/SNF2 family necessary for normal mitotic growth and transcription.

Authors:  P A Khavari; C L Peterson; J W Tamkun; D B Mendel; G R Crabtree
Journal:  Nature       Date:  1993-11-11       Impact factor: 49.962

7.  Functional interdependence of the yeast SNF2, SNF5, and SNF6 proteins in transcriptional activation.

Authors:  B C Laurent; M A Treitel; M Carlson
Journal:  Proc Natl Acad Sci U S A       Date:  1991-04-01       Impact factor: 11.205

8.  New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues.

Authors:  E Hochuli; H Döbeli; A Schacher
Journal:  J Chromatogr       Date:  1987-12-18

9.  The yeast ADR6 gene encodes homopolymeric amino acid sequences and a potential metal-binding domain.

Authors:  P J O'Hara; H Horowitz; G Eichinger; E T Young
Journal:  Nucleic Acids Res       Date:  1988-11-11       Impact factor: 16.971

10.  Altering the conserved nucleotide binding motif in the Salmonella typhimurium MutS mismatch repair protein affects both its ATPase and mismatch binding activities.

Authors:  L T Haber; G C Walker
Journal:  EMBO J       Date:  1991-09       Impact factor: 11.598
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  172 in total

1.  Cell cycle-regulated histone acetylation required for expression of the yeast HO gene.

Authors:  J E Krebs; M H Kuo; C D Allis; C L Peterson
Journal:  Genes Dev       Date:  1999-06-01       Impact factor: 11.361

2.  Fetal Alz-50 clone 1, a novel zinc finger protein, binds a specific DNA sequence and acts as a transcriptional regulator.

Authors:  K L Jordan-Sciutto; J M Dragich; J L Rhodes; R Bowser
Journal:  J Biol Chem       Date:  1999-12-03       Impact factor: 5.157

3.  Stability of a human SWI-SNF remodeled nucleosomal array.

Authors:  J R Guyon; G J Narlikar; E K Sullivan; R E Kingston
Journal:  Mol Cell Biol       Date:  2001-02       Impact factor: 4.272

4.  An in vitro system recapitulates chromatin remodeling at the PHO5 promoter.

Authors:  E S Haswell; E K O'Shea
Journal:  Mol Cell Biol       Date:  1999-04       Impact factor: 4.272

5.  Stable remodeling of tailless nucleosomes by the human SWI-SNF complex.

Authors:  J R Guyon; G J Narlikar; S Sif; R E Kingston
Journal:  Mol Cell Biol       Date:  1999-03       Impact factor: 4.272

6.  Tup1p represses Mcm1p transcriptional activation and chromatin remodeling of an a-cell-specific gene.

Authors:  I M Gavin; M P Kladde; R T Simpson
Journal:  EMBO J       Date:  2000-11-01       Impact factor: 11.598

7.  Set2 is a nucleosomal histone H3-selective methyltransferase that mediates transcriptional repression.

Authors:  Brian D Strahl; Patrick A Grant; Scott D Briggs; Zu-Wen Sun; James R Bone; Jennifer A Caldwell; Sahana Mollah; Richard G Cook; Jeffrey Shabanowitz; Donald F Hunt; C David Allis
Journal:  Mol Cell Biol       Date:  2002-03       Impact factor: 4.272

8.  Antagonistic remodelling by Swi-Snf and Tup1-Ssn6 of an extensive chromatin region forms the background for FLO1 gene regulation.

Authors:  A B Fleming; S Pennings
Journal:  EMBO J       Date:  2001-09-17       Impact factor: 11.598

9.  The nucleosome remodeling complex, Snf/Swi, is required for the maintenance of transcription in vivo and is partially redundant with the histone acetyltransferase, Gcn5.

Authors:  P Sudarsanam; Y Cao; L Wu; B C Laurent; F Winston
Journal:  EMBO J       Date:  1999-06-01       Impact factor: 11.598

10.  BRG1 requirement for long-range interaction of a locus control region with a downstream promoter.

Authors:  Shin-Il Kim; Scott J Bultman; Christine M Kiefer; Ann Dean; Emery H Bresnick
Journal:  Proc Natl Acad Sci U S A       Date:  2009-01-26       Impact factor: 11.205
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