Literature DB >> 796661

Overproduction of phage lambda repressor under control of the lac promotor of Escherichia coli.

B Gronenborn.   

Abstract

The gene coding for bacteriophage Lambda repressor (cI gene) has been fused to the lac operon of Escherichia coli. In some of the fusions Lambda repressor synthesis can be controlled by the lac operator and promoter. Upon induction of the lac operon the amount of Lambda repressor is increased by a factor of 7 over that found in a single lysogen. In combination with the polarity suppressor suA the induction factor rises to 20. Transducing phages of one fusion were constructed. After thermal induction of this phage the final level of Lambda repressor was enhanced by a factor of 150.

Entities:  

Mesh：

Substances：

Year:  1976        PMID： 796661     DOI： 10.1007/bf00332898

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  32 in total

1.  [Not Available].

Authors:  F JACOB; E L WOLLMAN
Journal:  Ann Inst Pasteur (Paris)       Date:  1954-12

2.  Genetic regulatory mechanisms in the synthesis of proteins.

Authors:  F JACOB; J MONOD
Journal:  J Mol Biol       Date:  1961-06       Impact factor: 5.469

3.  Sequence of the OR operator of phage lambda.

Authors:  V Pirrotta
Journal:  Nature       Date:  1975-03-13       Impact factor: 49.962

4.  Fusions of the lac and trp Regions of the Escherichia coli Chromosome.

Authors:  J H Miller; W S Reznikoff; A E Silverstone; K Ippen; E R Signer; J R Beckwith
Journal:  J Bacteriol       Date:  1970-12       Impact factor: 3.490

5.  A role for the product of gene suA in restoration of polarity in vitro.

Authors:  W Wetekam; R Ehring
Journal:  Mol Gen Genet       Date:  1973-08-28

6.  [Existence in Escherichia coli K 12 of a common regulation of the biosynthesis of bacteriophage receptors and maltose metabolism].

Authors:  M Schwartz
Journal:  Ann Inst Pasteur (Paris)       Date:  1967-11

Review 7.  Episomes.

Authors:  J Scaife
Journal:  Annu Rev Microbiol       Date:  1967       Impact factor: 15.500

8.  Control of short leftward transcripts from the immunity and ori regions in induced coliphage lambda.

Authors:  S Hayes; W Szybalski
Journal:  Mol Gen Genet       Date:  1973-11-22

9.  New mutations in the S cistron of bacteriophage lambda affecting host cell lysis.

Authors:  A R Goldberg; M Howe
Journal:  Virology       Date:  1969-05       Impact factor: 3.616

10.  Regulation of repressor expression in lambda.

Authors:  H Eisen; P Brachet; L Pereira da Silva; F Jacob
Journal:  Proc Natl Acad Sci U S A       Date:  1970-07       Impact factor: 11.205
View more
  21 in total

1.  DnaA dependent replication of plasmid R1 occurs in the presence of point mutations that disrupt the dnaA box of oriR.

Authors:  S Ortega-Jiménez; R Giraldo-Suárez; M E Fernández-Tresguerres; A Berzal-Herranz; R Díaz-Orejas
Journal:  Nucleic Acids Res       Date:  1992-05-25       Impact factor: 16.971

2.  EcoK selection vectors for shotgun cloning into M13 and deletion mutagenesis.

Authors:  M M Waye; M E Verhoeyen; P T Jones; G Winter
Journal:  Nucleic Acids Res       Date:  1985-12-09       Impact factor: 16.971

3.  Killing of Escherichia coli cells modulated by components of the stability system ParD of plasmid R1.

Authors:  A Bravo; S Ortega; G de Torrontegui; R Díaz
Journal:  Mol Gen Genet       Date:  1988-12

4.  Identification and Mutational Analysis of Escherichia coli Sorbitol-Enhanced Glucose-Repressed srlA Promoter Expressed in LB Medium by Using Homologous Recombination and One-Round PCR Products.

Authors:  Mikiko Nakamura; Junya Aihara; Hisashi Hoshida; Rinji Akada
Journal:  Mol Biotechnol       Date:  2018-12       Impact factor: 2.695

5.  Isolation and characterization of a conditional replication mutant of the antibiotic resistance factor R1 affected in the gene of the replication protein repA.

Authors:  S Ortega; G de Torrontegui; R Díaz
Journal:  Mol Gen Genet       Date:  1989-05

6.  Lambda phage promoter used to enhance expression of a plasmid-cloned gene.

Authors:  J Hedgpeth; M Ballivet; H Eisen
Journal:  Mol Gen Genet       Date:  1978-07-11

7.  Filamentous coliphage M13 as a cloning vehicle: insertion of a HindII fragment of the lac regulatory region in M13 replicative form in vitro.

Authors:  J Messing; B Gronenborn; B Müller-Hill; P Hans Hopschneider
Journal:  Proc Natl Acad Sci U S A       Date:  1977-09       Impact factor: 11.205

8.  Structural bases of a long-stretched deletion: completing the lambda plac5 DNA primary structure.

Authors:  G V Shpakovski; A A Akhrem
Journal:  Nucleic Acids Res       Date:  1988-11-11       Impact factor: 16.971

9.  Chimeric transcriptional activators generated in vivo from VnfA and AnfA of Azotobacter vinelandii: N-terminal domain of AnfA is responsible for dependence on nitrogenase Fe protein.

Authors:  E Frise; A Green; M Drummond
Journal:  J Bacteriol       Date:  1994-11       Impact factor: 3.490

Review 10.  Strategies for achieving high-level expression of genes in Escherichia coli.

Authors:  S C Makrides
Journal:  Microbiol Rev       Date:  1996-09
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.