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Literature DB >> 16559103

Fusions of the lac and trp Regions of the Escherichia coli Chromosome.

J H Miller¹, W S Reznikoff, A E Silverstone, K Ippen, E R Signer, J R Beckwith.

Abstract

Two classes of strains were studied in which the lac operon is transposed to a chromosomal site close to the tonB and trp loci. The two classes differ in the orientation of the lac region on the chromosome. In both types of strains, tonB mutants were selected in which deletions removing the tonB locus also caused a fusion of the lac and trp regions. The study of the properties of such fusion strains provides information on the control of both the lac and trp operons.

Entities: Chemical Species

Year: 1970 PMID： 16559103 PMCID： PMC248287 DOI： 10.1128/jb.104.3.1273-1279.1970

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

25 in total

1. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

2. The promoter-operator region of the lac operon of Escherichia coli.

Authors: J H Miller; K Ippen; J G Scaife; J R Beckwith
Journal: J Mol Biol Date: 1968-12 Impact factor: 5.469

3. Direction of transcription of a regulatory gene in E. coli.

Authors: J H Miller; J Beckwith; B Muller-Hill
Journal: Nature Date: 1968-12-28 Impact factor: 49.962

4. The non-linear relationship between the enzyme activity and structural protein concentration of thiogalactoside transacetylase of E. coli.

Authors: C A Michels; D Zipser
Journal: Biochem Biophys Res Commun Date: 1969-02-21 Impact factor: 3.575

5. Polarity in the tryptophan operon of E. coli.

Authors: F Imamoto; J Ito; C Yanofsky
Journal: Cold Spring Harb Symp Quant Biol Date: 1966

6. Transposition of the Lac region of E. coli.

Authors: J R Beckwith; E R Signer; W Epstein
Journal: Cold Spring Harb Symp Quant Biol Date: 1966

Review 7. Revised linkage map of Escherichia coli.

Authors: A L Taylor; C D Trotter
Journal: Bacteriol Rev Date: 1967-12

8. New controlling element in the Lac operon of E. coli.

Authors: K Ippen; J H Miller; J Scaife; J Beckwith
Journal: Nature Date: 1968-03-02 Impact factor: 49.962

9. Consequences of deletion mutations joining two operons of opposite polarity.

Authors: M Levinthal; H Nikaido
Journal: J Mol Biol Date: 1969-06-28 Impact factor: 5.469

10. Direction of in vivo degradation of tryptophan messenger RNA--a correction.

Authors: D E Morse; R Mosteller; R F Baker; C Yanofsky
Journal: Nature Date: 1969-07-05 Impact factor: 49.962

32 in total

1. Fusion of the Escherichia coli lac genes to the ara promoter: a general technique using bacteriophage Mu-1 insertions.

Authors: M J Casadaban
Journal: Proc Natl Acad Sci U S A Date: 1975-03 Impact factor: 11.205

9. Production of a functional eukaryotic enzyme in Escherichia coli: cloning and expression of the yeast structural gene for imidazole-glycerolphosphate dehydratase (his3).

Authors: K Struhl; R W Davis
Journal: Proc Natl Acad Sci U S A Date: 1977-12 Impact factor: 11.205

10. Isolation and characterization of mutations creating high-efficiency transcription initiation signals within the trp operon of Escherichia coli.

Authors: A McPartland; R L Somerville
Journal: J Bacteriol Date: 1976-11 Impact factor: 3.490

Fusions of the lac and trp Regions of the Escherichia coli Chromosome.

1. Protein measurement with the Folin phenol reagent.

2. The promoter-operator region of the lac operon of Escherichia coli.

3. Direction of transcription of a regulatory gene in E. coli.

4. The non-linear relationship between the enzyme activity and structural protein concentration of thiogalactoside transacetylase of E. coli.

5. Polarity in the tryptophan operon of E. coli.

6. Transposition of the Lac region of E. coli.

Review 7. Revised linkage map of Escherichia coli.

8. New controlling element in the Lac operon of E. coli.

9. Consequences of deletion mutations joining two operons of opposite polarity.

10. Direction of in vivo degradation of tryptophan messenger RNA--a correction.

1. Fusion of the Escherichia coli lac genes to the ara promoter: a general technique using bacteriophage Mu-1 insertions.

Review 2. Uses of lac fusions for the study of biological problems.

3. A toolkit for DNA assembly, genome engineering and multicolor imaging for C. elegans.

4. Lambda placMu: a transposable derivative of bacteriophage lambda for creating lacZ protein fusions in a single step.

5. Positioning flagellar genes in Escherichia coli by deletion analysis.

6. Overproduction of phage lambda repressor under control of the lac promotor of Escherichia coli.

7. Isolating tryptophan regulatory mutants in Escherichia coli by using a trp-lac fusion strain.

8. Bacteriophage P1 as a vehicle for Mu mutagenesis of Salmonella typhimurium.

9. Production of a functional eukaryotic enzyme in Escherichia coli: cloning and expression of the yeast structural gene for imidazole-glycerolphosphate dehydratase (his3).

10. Isolation and characterization of mutations creating high-efficiency transcription initiation signals within the trp operon of Escherichia coli.