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Literature DB >> 355849

Lambda phage promoter used to enhance expression of a plasmid-cloned gene.

Abstract

A 50-fold (or greater) increase in the production of phage 21 repressor was obtained by construction of a plasmid in which the 21cI (repressor) gene could be transcribed from lambdaPL. The enhancement due to increased 21cI gene copy number and transcription from lambdaPL were at least five-fold and ten-fold, respectively. The plasmid was constructed in vitro by recombination of EcoRI-generated DNA fragments. The use of the DNA fragment containing lambdaPL in obtaining expression of cloned genes is discussed.

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Year: 1978 PMID： 355849 DOI： 10.1007/bf00267410

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

28 in total

1. [On a thermosensitive repression system in the Escherichia coli lambda bacteriophage].

Authors: R SUSSMAN; F JACOB
Journal: C R Hebd Seances Acad Sci Date: 1962-02-19

2. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

Authors: P A Sharp; B Sugden; J Sambrook
Journal: Biochemistry Date: 1973-07-31 Impact factor: 3.162

3. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.

Authors: D B Clewell
Journal: J Bacteriol Date: 1972-05 Impact factor: 3.490

4. Control of short leftward transcripts from the immunity and ori regions in induced coliphage lambda.

Authors: S Hayes; W Szybalski
Journal: Mol Gen Genet Date: 1973-11-22

5. Mutations of coliphage lambda affecting the expression of replicative functions O and P.

Authors: P Brachet; H Eisen; A Rambach
Journal: Mol Gen Genet Date: 1970

6. Regulation of repressor expression in lambda.

Authors: H Eisen; P Brachet; L Pereira da Silva; F Jacob
Journal: Proc Natl Acad Sci U S A Date: 1970-07 Impact factor: 11.205

7. Positive control of endolysin synthesis in vitro by the gene N protein of phage lambda.

Authors: J Greenblatt
Journal: Proc Natl Acad Sci U S A Date: 1972-12 Impact factor: 11.205

8. Overproduction of phage lambda repressor under control of the lac promotor of Escherichia coli.

Authors: B Gronenborn
Journal: Mol Gen Genet Date: 1976-11-17

9. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.

Authors: S N Cohen; A C Chang; L Hsu
Journal: Proc Natl Acad Sci U S A Date: 1972-08 Impact factor: 11.205

10. Release of polarity in Escherichia coli by gene N of phage lambda: termination and antitermination of transcription.

Authors: S Adhya; M Gottesman; B De Crombrugghe
Journal: Proc Natl Acad Sci U S A Date: 1974-06 Impact factor: 11.205

7 in total

1. Origin and binding specificity of protein(s) coded for by Mu prophages.

Authors: W Schumann; C Westphal; E G Bade; L Holzer
Journal: Mol Gen Genet Date: 1979-06-07

2. Analysis of lambda receptor and beta-lactamase synthesis and export using cloned genes in a minicell system.

Authors: J M Clement; D Perrin; J Hedgpeth
Journal: Mol Gen Genet Date: 1982

3. Cloning and functional characterization of the plasmid-encoded hemolysin determinant of Escherichia coli.

Authors: W Goebel; J Hedgpeth
Journal: J Bacteriol Date: 1982-09 Impact factor: 3.490

4. DNA sequence encoding the NH2-terminal peptide involved in transport of lambda receptor, an Escherichia coli secretory protein.

Authors: J Hedgpeth; J M Clement; C Marchal; D Perrin; M Hofnung
Journal: Proc Natl Acad Sci U S A Date: 1980-05 Impact factor: 11.205