Literature DB >> 7861968

Immunochemical quantitation of bromodeoxyuridine: application to cell-cycle kinetics.

F Dolbeare1, J R Selden.   

Abstract

We have described several laboratory procedures for the immunochemical staining of the halopyrimidines, BrdUrd and IdUrd, in cell suspensions for flow cytometry and a method for staining histological sections on slides. Halogenated pyrimidine quantitation allows cell-cycle parameters, including total cell-cycle time, phase durations, and growth fraction to be determined. We have presented some flow cytometric data to demonstrate the use of these methods in determining bivariate BrdUrd/DNA histograms with CHO cells and in kinetic studies with the brown Norway rat myeloid leukemia model.

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Year:  1994        PMID： 7861968

Source DB:  PubMed          Journal:  Methods Cell Biol        ISSN： 0091-679X            Impact factor:   1.441


  15 in total

1.  Spatial patterns of DNA replication, protein synthesis, and oxygen concentration within bacterial biofilms reveal diverse physiological states.

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Review 2.  Cytometry of ATM activation and histone H2AX phosphorylation to estimate extent of DNA damage induced by exogenous agents.

Authors:  Toshiki Tanaka; Xuan Huang; H Dorota Halicka; Hong Zhao; Frank Traganos; Anthony P Albino; Wei Dai; Zbigniew Darzynkiewicz
Journal:  Cytometry A       Date:  2007-09       Impact factor: 4.355

3.  Scleractinian coral cell proliferation is reduced in primary culture of suspended multicellular aggregates compared to polyps.

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Journal:  Cytotechnology       Date:  2013-06-12       Impact factor: 2.058

4.  Detection of DNA strand breaks in apoptotic cells by flow- and image-cytometry.

Authors:  Zbigniew Darzynkiewicz; Hong Zhao
Journal:  Methods Mol Biol       Date:  2011

5.  Type III secretion-dependent cell cycle block caused in HeLa cells by enteropathogenic Escherichia coli O103.

Authors:  J P Nougayrède; M Boury; C Tasca; O Marchès; A Milon; E Oswald; J De Rycke
Journal:  Infect Immun       Date:  2001-11       Impact factor: 3.441

6.  Systems biology in cancer research: genomics to cellomics.

Authors:  Jackie L Stilwell; Yinghui Guan; Richard M Neve; Joe W Gray
Journal:  Methods Mol Biol       Date:  2007

Review 7.  Cytometry of DNA replication and RNA synthesis: Historical perspective and recent advances based on "click chemistry".

Authors:  Zbigniew Darzynkiewicz; Frank Traganos; Hong Zhao; H Dorota Halicka; Jiangwei Li
Journal:  Cytometry A       Date:  2011-03-18       Impact factor: 4.355

8.  Kinetics of the UV-induced DNA damage response in relation to cell cycle phase. Correlation with DNA replication.

Authors:  Hong Zhao; Frank Traganos; Zbigniew Darzynkiewicz
Journal:  Cytometry A       Date:  2010-03       Impact factor: 4.355

9.  Effects of aging on thyroarytenoid muscle regeneration.

Authors:  Kyungah Lee; Heidi Kletzien; Nadine P Connor; Edward Schultz; Connie S Chamberlain; Diane M Bless
Journal:  Laryngoscope       Date:  2012-09-10       Impact factor: 3.325

10.  Analysis of apoptosis by cytometry using TUNEL assay.

Authors:  Zbigniew Darzynkiewicz; Dariusz Galkowski; Hong Zhao
Journal:  Methods       Date:  2008-03       Impact factor: 3.608
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