Literature DB >> 7804006

The elimination of O-linked glycans from glycoproteins under non-reducing conditions.

C A Cooper1, N H Packer, J W Redmond.   

Abstract

A simple procedure is described for the elimination of O-linked glycans from bovine submaxillary mucin under non-reducing conditions, using triethylamine in aqueous hydrazine. The glycans were isolated as the hydrazones, which were converted to the reducing glycans by exchange with acetone in neutral aqueous solution. The glycan alditols obtained after reduction corresponded to those obtained by the reductive beta-elimination of O-glycans.

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Year:  1994        PMID： 7804006     DOI： 10.1007/bf00731156

Source DB:  PubMed          Journal:  Glycoconj J        ISSN： 0282-0080            Impact factor:   2.916


  12 in total

1.  Purification and properties of an endo-alpha-N-acetyl-D-galactosaminidase from Diplococcus pneumoniae.

Authors:  J Umemoto; V P Bhavanandan; E A Davidson
Journal:  J Biol Chem       Date:  1977-12-10       Impact factor: 5.157

2.  Hydrazinolysis of asparagine-linked sugar chains to produce free oligosaccharides.

Authors:  S Takasaki; T Mizuochi; A Kobata
Journal:  Methods Enzymol       Date:  1982       Impact factor: 1.600

3.  Action of alkali on bovine and ovine submaxillary mucins.

Authors:  M Bertolini; W Pigman
Journal:  J Biol Chem       Date:  1967-09-10       Impact factor: 5.157

4.  The effect of alkaline borohydride treatment on N-linked carbohydrates of glycoproteins.

Authors:  S P Argade; G D Daves; H Van Halbeek; J A Alhadeff
Journal:  Glycoconj J       Date:  1989       Impact factor: 2.916

5.  Mild alkaline borohydride treatment of glycoproteins-a method for liberating both N- and O-linked carbohydrate chains.

Authors:  S Ogata; K O Lloyd
Journal:  Anal Biochem       Date:  1982-01-15       Impact factor: 3.365

6.  Reductive cleavage of Xaa-proline peptide bonds by mild alkaline borohydride treatment employed to release O-glycosidically linked carbohydrate units of glycoproteins.

Authors:  M Shimamura; Y Inoue; S Inoue
Journal:  Arch Biochem Biophys       Date:  1984-08-01       Impact factor: 4.013

7.  STUDIES ON GLYCOPROTEINS. XI. THE O-GLYCOSIDIC LINKAGE OF N-ACETYLGALACTOSAMINE TO SERYL AND THREONYL RESIDUES IN OVINE SUBMAXILLARY GLAND GLYCOPROTEIN.

Authors:  R CARUBELLI; P BHAVANANDAN; A GOTTSCHALK
Journal:  Biochim Biophys Acta       Date:  1965-03-01

8.  Use of hydrazine to release in intact and unreduced form both N- and O-linked oligosaccharides from glycoproteins.

Authors:  T Patel; J Bruce; A Merry; C Bigge; M Wormald; A Jaques; R Parekh
Journal:  Biochemistry       Date:  1993-01-19       Impact factor: 3.162

9.  The effect of mild alkali and alkaline borohydride on the carbohydrate and peptide moieties of fetuin.

Authors:  E F Hounsell; N J Pickering; M S Stoll; A M Lawson; T Feizi
Journal:  Biochem Soc Trans       Date:  1984-08       Impact factor: 5.407

10.  Hydrazinolysis-N-reacetylation of glycopeptides and glycoproteins. Model studies using 2-acetamido-1-N-(L-aspart-4-oyl)-2-deoxy-beta-D-glucopyranosy lamine.

Authors:  B Bendiak; D A Cumming
Journal:  Carbohydr Res       Date:  1985-11-15       Impact factor: 2.104
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  5 in total

1.  Negative ion CID fragmentation of O-linked oligosaccharide aldoses--charge induced and charge remote fragmentation.

Authors:  Roisin A Doohan; Catherine A Hayes; Brendan Harhen; Niclas Göran Karlsson
Journal:  J Am Soc Mass Spectrom       Date:  2011-03-31       Impact factor: 3.109

2.  Probing mucin-type O-linked glycosylation in living animals.

Authors:  Danielle H Dube; Jennifer A Prescher; Chi N Quang; Carolyn R Bertozzi
Journal:  Proc Natl Acad Sci U S A       Date:  2006-03-20       Impact factor: 11.205

3.  LC-MS3 quantification of O-glycopeptides in human serum.

Authors:  Miloslav Sanda; Petr Pompach; Julius Benicky; Radoslav Goldman
Journal:  Electrophoresis       Date:  2013-07-24       Impact factor: 3.535

4.  LW-AFC Effects on N-glycan Profile in Senescence-Accelerated Mouse Prone 8 Strain, a Mouse Model of Alzheimer's Disease.

Authors:  Jianhui Wang; Xiaorui Cheng; Ju Zeng; Jiangbei Yuan; Zhongfu Wang; Wenxia Zhou; Yongxiang Zhang
Journal:  Aging Dis       Date:  2017-02-01       Impact factor: 6.745

5.  Application of the 50% Hydrazine Solution Method for O-Glycans Release, their Chemical Labeling, and HPLC Separation.

Authors:  D G Kisiel; I Radziejewska; A Gindzieński
Journal:  Toxicol Mech Methods       Date:  2008-06-23       Impact factor: 2.987
  5 in total

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