Literature DB >> 7765770

Construction of a new host-vector system in Arthrobacter sp. and cloning of the lipase gene.

M Morikawa1, H Daido, S Pongpobpibool, T Imanaka.   

Abstract

Arthrobacter sp. strain MIS38 was transformed with a shuttle vector containing the kanamycin resistant gene kan (derived from Tn5) by an electroporation method. This shuttle vector is from Brevibacterium lactofermentum and Escherichia coli, pULRS8. The following optimal condition of electroporation was determined. A square wave pulse of 1 kV/cm electric field strength for 0.5 ms duration yielded 3 x 10(5) transformants/micrograms plasmid DNA. The number of transformants increased with the amount of DNA over the range 0.01-5 micrograms. This host-vector system was then used successfully to clone and express a lipase gene from Arthrobacter sp. strain MIS38 into both Arthrobacter sp. MIS38 and E. coli JM109.

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Year:  1994        PMID： 7765770     DOI： 10.1007/BF00902732

Source DB:  PubMed          Journal:  Appl Microbiol Biotechnol        ISSN： 0175-7598            Impact factor:   4.813


  7 in total

1.  High efficiency transformation of E. coli by high voltage electroporation.

Authors:  W J Dower; J F Miller; C W Ragsdale
Journal:  Nucleic Acids Res       Date:  1988-07-11       Impact factor: 16.971

2.  Electroporation for the efficient transfection of mammalian cells with DNA.

Authors:  G Chu; H Hayakawa; P Berg
Journal:  Nucleic Acids Res       Date:  1987-02-11       Impact factor: 16.971

3.  Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.

Authors:  C Yanisch-Perron; J Vieira; J Messing
Journal:  Gene       Date:  1985       Impact factor: 3.688

4.  A new lipopeptide biosurfactant produced by Arthrobacter sp. strain MIS38.

Authors:  M Morikawa; H Daido; T Takao; S Murata; Y Shimonishi; T Imanaka
Journal:  J Bacteriol       Date:  1993-10       Impact factor: 3.490

5.  High-frequency transformation of Brevibacterium lactofermentum protoplasts by plasmid DNA.

Authors:  R I Santamaria; J A Gil; J F Martin
Journal:  J Bacteriol       Date:  1985-04       Impact factor: 3.490

6.  Cloning vector system for Corynebacterium glutamicum.

Authors:  M Yoshihama; K Higashiro; E A Rao; M Akedo; W G Shanabruch; M T Follettie; G C Walker; A J Sinskey
Journal:  J Bacteriol       Date:  1985-05       Impact factor: 3.490

7.  Cloning of the genes for penicillinase, penP and penI, of Bacillus licheniformis in some vector plasmids and their expression in Escherichia coli, Bacillus subtilis, and Bacillus licheniformis.

Authors:  T Imanaka; T Tanaka; H Tsunekawa; S Aiba
Journal:  J Bacteriol       Date:  1981-09       Impact factor: 3.490
  7 in total
  3 in total

1.  Plasmids for nicotine-dependent and -independent gene expression in Arthrobacter nicotinovorans and other arthrobacter species.

Authors:  Cristinel Sandu; Calin-Bogdan Chiribau; Paula Sachelaru; Roderich Brandsch
Journal:  Appl Environ Microbiol       Date:  2005-12       Impact factor: 4.792

2.  Characterization of a cryptic plasmid from a Greenland ice core Arthrobacter isolate and construction of a shuttle vector that replicates in psychrophilic high G+C Gram-positive recipients.

Authors:  Vanya Miteva; Sarah Lantz; Jean Brenchley
Journal:  Extremophiles       Date:  2008-03-12       Impact factor: 2.395

3.  Gene cloning, heterologous expression, and partial characterization of a novel cold-adapted subfamily I.3 lipase from Pseudomonas fluorescence KE38.

Authors:  Fulya Karakaş; Alper Arslanoğlu
Journal:  Sci Rep       Date:  2020-12-16       Impact factor: 4.379
  3 in total

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