Literature DB >> 3980445

High-frequency transformation of Brevibacterium lactofermentum protoplasts by plasmid DNA.

R I Santamaria, J A Gil, J F Martin.   

Abstract

An efficient polyethylene glycol-assisted method for transformation of Brevibacterium lactofermentum protoplasts that uses plasmid vectors has been developed. Two small plasmids, pUL330 (5.2 kilobases) and pUL340 (5.8 kilobases), both containing the kanamycin resistance gene from transposon Tn5 and the replication origin of the natural plasmid pBL1 of B. lactofermentum, were selected as vectors. Supercoiled forms of the plasmids yielded a 100-fold higher transformation frequency than did linear forms. The optimal transformation frequency was achieved with 10 ng of DNA in 1 ml of transformation buffer. Higher concentrations of plasmid DNA resulted in a decrease in transformation frequency per microgram of DNA. Optimal transformation was obtained with 25 to 35% polyethylene glycol 6000. Under optimal conditions, 10(6) transformants per microgram of DNA were obtained.

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Year:  1985        PMID： 3980445      PMCID： PMC219017          DOI： 10.1128/jb.162.1.463-467.1985

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  7 in total

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Authors:  K F Chater; D A Hopwood; T Kieser; C J Thompson
Journal:  Curr Top Microbiol Immunol       Date:  1982       Impact factor: 4.291

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Authors:  M J Bibb; J M Ward; D A Hopwood
Journal:  Nature       Date:  1978-07-27       Impact factor: 49.962

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Authors:  D A Hopwood
Journal:  Annu Rev Microbiol       Date:  1981       Impact factor: 15.500

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Authors:  T A Trautner; H C Spatz
Journal:  Curr Top Microbiol Immunol       Date:  1973       Impact factor: 4.291

5.  High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA.

Authors:  S Chang; S N Cohen
Journal:  Mol Gen Genet       Date:  1979-01-05

6.  Polyethylene glycol-assisted transfection of Streptomyces protoplasts.

Authors:  J E Suarez; K F Chater
Journal:  J Bacteriol       Date:  1980-04       Impact factor: 3.490

7.  Plasmid transformation in Bacillus subtilis: effects of insertion of Bacillus subtilis DNA into plasmid pC194.

Authors:  U Canosi; A Iglesias; T A Trautner
Journal:  Mol Gen Genet       Date:  1981
  7 in total
  24 in total

1.  D-Pantothenate synthesis in Corynebacterium glutamicum and use of panBC and genes encoding L-valine synthesis for D-pantothenate overproduction.

Authors:  H Sahm; L Eggeling
Journal:  Appl Environ Microbiol       Date:  1999-05       Impact factor: 4.792

2.  The bleomycin resistance gene of transposon Tn5 is an excellent marker for transformation of corynebacteria.

Authors:  C Guerrero; L M Mateos; M Malumbres; J F Martín
Journal:  Appl Microbiol Biotechnol       Date:  1992-03       Impact factor: 4.813

3.  Efficient Transformation of the Cephamycin C Producer Nocardia lactamdurans and Development of Shuttle and Promoter-Probe Cloning Vectors.

Authors:  C V Kumar; J J Coque; J F Martín
Journal:  Appl Environ Microbiol       Date:  1994-11       Impact factor: 4.792

4.  Phosphorylation of a novel cytoskeletal protein (RsmP) regulates rod-shaped morphology in Corynebacterium glutamicum.

Authors:  Maria Fiuza; Michal Letek; Jade Leiba; Almudena F Villadangos; José Vaquera; Isabelle Zanella-Cléon; Luís M Mateos; Virginie Molle; José A Gil
Journal:  J Biol Chem       Date:  2010-07-09       Impact factor: 5.157

5.  Genetic transfers in Brevibacterium sp.

Authors:  V Rytír; A Sroglová; I Holubová; M Konícková-Radochová; J Konícek
Journal:  Folia Microbiol (Praha)       Date:  1986       Impact factor: 2.099

6.  Transcriptional analysis and regulatory signals of the hom-thrB cluster of Brevibacterium lactofermentum.

Authors:  L M Mateos; A Pisabarro; M Pátek; M Malumbres; C Guerrero; B J Eikmanns; H Sahm; J F Martín
Journal:  J Bacteriol       Date:  1994-12       Impact factor: 3.490

7.  Characterization of a region of plasmid pBL1 of Brevibacterium lactofermentum involved in replication via the rolling circle model.

Authors:  C Fernandez-Gonzalez; R F Cadenas; M F Noirot-Gros; J F Martin; J A Gil
Journal:  J Bacteriol       Date:  1994-06       Impact factor: 3.490

8.  DivIVA is required for polar growth in the MreB-lacking rod-shaped actinomycete Corynebacterium glutamicum.

Authors:  Michal Letek; Efrén Ordóñez; José Vaquera; William Margolin; Klas Flärdh; Luis M Mateos; José A Gil
Journal:  J Bacteriol       Date:  2008-02-22       Impact factor: 3.490

9.  Integration of narrow-host-range vectors from Escherichia coli into the genomes of amino acid-producing corynebacteria after intergeneric conjugation.

Authors:  L M Mateos; A Schäfer; J Kalinowski; J F Martin; A Pühler
Journal:  J Bacteriol       Date:  1996-10       Impact factor: 3.490

10.  A gene encoding arginyl-tRNA synthetase is located in the upstream region of the lysA gene in Brevibacterium lactofermentum: regulation of argS-lysA cluster expression by arginine.

Authors:  J A Oguiza; M Malumbres; G Eriani; A Pisabarro; L M Mateos; F Martin; J F Martín
Journal:  J Bacteriol       Date:  1993-11       Impact factor: 3.490
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