| Literature DB >> 16332890 |
Cristinel Sandu1, Calin-Bogdan Chiribau, Paula Sachelaru, Roderich Brandsch.
Abstract
The first inducible Arthrobacter overexpression system, based on the promoter/operator and the repressor of the 6-D-hydroxynicotine oxidase gene of Arthrobacter nicotinovorans, is described here. Nicotine-dependent overproduction and affinity purification of recombinant proteins are presented. The system will allow the production of complex enzymes and genetic complementation studies in Arthrobacter species.Entities:
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Year: 2005 PMID: 16332890 PMCID: PMC1317448 DOI: 10.1128/AEM.71.12.8920-8924.2005
Source DB: PubMed Journal: Appl Environ Microbiol ISSN: 0099-2240 Impact factor: 4.792