Literature DB >> 3029703

Electroporation for the efficient transfection of mammalian cells with DNA.

G Chu, H Hayakawa, P Berg.   

Abstract

A simple and reproducible procedure for the introduction of DNA into mammalian cells by electroporation is described. The parameters involving the cells, the DNA, and the electric field are investigated. The procedure has been applied to a broad range of animal cells. It is capable of transforming more than 1% of the viable cells to the stable expression of a selectable marker.

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Year:  1987        PMID： 3029703      PMCID： PMC340526          DOI： 10.1093/nar/15.3.1311

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  28 in total

1.  Defective simian virus 40 genomes: isolation and growth of individual clones.

Authors:  J E Mertz; P Berg
Journal:  Virology       Date:  1974-11       Impact factor: 3.616

2.  A new technique for the assay of infectivity of human adenovirus 5 DNA.

Authors:  F L Graham; A J van der Eb
Journal:  Virology       Date:  1973-04       Impact factor: 3.616

3.  SV40 DNA transfection of cells in suspension: analysis of efficiency of transcription and translation of T-antigen.

Authors:  G Chu; P A Sharp
Journal:  Gene       Date:  1981-03       Impact factor: 3.688

4.  Mapping temperature-sensitive and host-range mutations of adenovirus type 5 by marker rescue.

Authors:  E Frost; J Williams
Journal:  Virology       Date:  1978-11       Impact factor: 3.616

5.  Voltage-induced pore formation and hemolysis of human erythrocytes.

Authors:  K Kinosita; T Y Tsong
Journal:  Biochim Biophys Acta       Date:  1977-12-01

6.  Human growth hormone as a reporter gene in regulation studies employing transient gene expression.

Authors:  R F Selden; K B Howie; M E Rowe; H M Goodman; D D Moore
Journal:  Mol Cell Biol       Date:  1986-09       Impact factor: 4.272

7.  Coordinate overproduction of orotate phosphoribosyltransferase and orotidine-5'-phosphate decarboxylase in hamster cells resistant to pyrazofurin and 6-azauridine.

Authors:  D P Suttle; G R Stark
Journal:  J Biol Chem       Date:  1979-06-10       Impact factor: 5.157

8.  Direct transfer of cloned genes from bacteria to mammalian cells.

Authors:  W Schaffner
Journal:  Proc Natl Acad Sci U S A       Date:  1980-04       Impact factor: 11.205

9.  An improved technique for obtaining enhanced infectivity with herpes simplex virus type 1 DNA.

Authors:  N D Stow; N M Wilkie
Journal:  J Gen Virol       Date:  1976-12       Impact factor: 3.891

10.  Phage particle-mediated gene transfer to cultured mammalian cells.

Authors:  M Ishiura; S Hirose; T Uchida; Y Hamada; Y Suzuki; Y Okada
Journal:  Mol Cell Biol       Date:  1982-06       Impact factor: 4.272
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  250 in total

1.  Membrane-anchored metalloprotease MDC9 has an alpha-secretase activity responsible for processing the amyloid precursor protein.

Authors:  H Koike; S Tomioka; H Sorimachi; T C Saido; K Maruyama; A Okuyama; A Fujisawa-Sehara; S Ohno; K Suzuki; S Ishiura
Journal:  Biochem J       Date:  1999-10-15       Impact factor: 3.857

2.  CD147 is tightly associated with lactate transporters MCT1 and MCT4 and facilitates their cell surface expression.

Authors:  P Kirk; M C Wilson; C Heddle; M H Brown; A N Barclay; A P Halestrap
Journal:  EMBO J       Date:  2000-08-01       Impact factor: 11.598

3.  Consequences of gene targeting procedures for behavioural responses and morphological development of newborn mice.

Authors:  M van der Meer; V Baumans; F M Hofhuis; B Olivier; B F van Zutphen
Journal:  Transgenic Res       Date:  2001-10       Impact factor: 2.788

4.  Regulation of the Raf-1 kinase domain by phosphorylation and 14-3-3 association.

Authors:  M T Yip-Schneider; W Miao; A Lin; D S Barnard; G Tzivion; M S Marshall
Journal:  Biochem J       Date:  2000-10-01       Impact factor: 3.857

5.  Efficient DNA transfection in neuronal and astrocytic cell lines.

Authors:  C Ghosh; W Song; D K Lahiri
Journal:  Mol Biol Rep       Date:  2000-06       Impact factor: 2.316

6.  Development of murine embryos following electroporation.

Authors:  C A Schmotzer; M E Dunlap-Brown; S P Butler; W H Velander; F C Gwazdauskas
Journal:  J Assist Reprod Genet       Date:  2003-04       Impact factor: 3.412

7.  Electroporation and electrophoretic DNA transfer into cells. The effect of DNA interaction with electropores.

Authors:  S I Sukharev; V A Klenchin; S M Serov; L V Chernomordik
Journal:  Biophys J       Date:  1992-11       Impact factor: 4.033

8.  Transfected rat islet tumour cells express mouse major histocompatibility complex class I antigens functionally. Applicable as "pseudo-syngeneic" targets in the multiple low-dose streptozotocin diabetes model.

Authors:  P Serup; J Schøller
Journal:  Diabetologia       Date:  1989-07       Impact factor: 10.122

9.  Specific gene suppression by engineered ribozymes in monkey cells.

Authors:  F H Cameron; P A Jennings
Journal:  Proc Natl Acad Sci U S A       Date:  1989-12       Impact factor: 11.205

10.  An arginine to glutamine mutation in residue 109 of human ornithine transcarbamylase completely abolishes enzymatic activity in Cos1 cells.

Authors:  J T Lee; R L Nussbaum
Journal:  J Clin Invest       Date:  1989-12       Impact factor: 14.808
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