| Literature DB >> 7761468 |
W Cai1, H Aburatani, V P Stanton, D E Housman, Y K Wang, D C Schwartz.
Abstract
We have developed a surface mounting technology for the rapid construction of ordered restriction maps from individual DNA molecules. Optical restriction maps constructed from yeast artificial chromosome DNA molecules mounted on specially derivatized glass surfaces are accurate and reproducible, and the technology is amenable to automation. The mounting procedures described here should also be useful for fluorescence in situ hybridization studies. We believe these improvements to optical mapping will further stimulate the development of nonelectrophoretic approaches to genome analysis.Entities:
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Year: 1995 PMID: 7761468 PMCID: PMC41869 DOI: 10.1073/pnas.92.11.5164
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN: 0027-8424 Impact factor: 11.205