Literature DB >> 7686265

The single cell gel electrophoresis assay (comet assay): a European review.

V J McKelvey-Martin1, M H Green, P Schmezer, B L Pool-Zobel, M P De Méo, A Collins.   

Abstract

The single cell gel electrophoresis (SCGE) assay is a rapid, simple, visual and sensitive technique for measuring DNA breakage in individual mammalian cells. Here we review the development of the SCGE assay (with particular reference to the alkaline version), existing protocols for the detection and analysis of comets, the relevant underlying principles determining the behaviour of DNA, and the potential applications of the technique.

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Year:  1993        PMID： 7686265     DOI： 10.1016/0027-5107(93)90207-v

Source DB:  PubMed          Journal:  Mutat Res        ISSN： 0027-5107            Impact factor:   2.433


  74 in total

1.  Updating quality control assays in the assisted reproductive technologies laboratory with a cryopreserved hamster oocyte DNA cytogenotoxic assay.

Authors:  P J Chan; J H Calinisan; J U Corselli; W C Patton; A King
Journal:  J Assist Reprod Genet       Date:  2001-03       Impact factor: 3.412

2.  Cryopreservation of human cumulus cells for co-cultures and assessment of DNA damage after thawing using the comet assay.

Authors:  E M Lindley; J D Jacobson; J Corselli; A King; P J Chan
Journal:  J Assist Reprod Genet       Date:  2001-10       Impact factor: 3.412

3.  Cytoprotective effect of hyaluronic acid and hydroxypropyl methylcellulose against DNA damage induced by thimerosal in Chang conjunctival cells.

Authors:  Juan Ye; Huina Zhang; Han Wu; Changjun Wang; Xin Shi; Jiajun Xie; Jinjing He; Jun Yang
Journal:  Graefes Arch Clin Exp Ophthalmol       Date:  2012-06-24       Impact factor: 3.117

4.  DNA damage in male gonad cells of Green mussel (Perna viridis) upon exposure to tobacco products.

Authors:  Anutosh Ganguly; Usha Goswami
Journal:  Ecotoxicology       Date:  2006-05-04       Impact factor: 2.823

5.  Genotoxicity and cytotoxicity of glass ionomer cements on Chinese hamster ovary (CHO) cells.

Authors:  Daniel Araki Ribeiro; Mariangela Esther Alencar Marques; Daisy Maria Favero Salvadori
Journal:  J Mater Sci Mater Med       Date:  2006-06       Impact factor: 3.896

6.  No increased DNA damage in peripheral lymphocytes of sewage workers as evaluated by alkaline single cell gel electrophoresis.

Authors:  L Friis; H Vaghef; C Edling; B Hellman
Journal:  Occup Environ Med       Date:  1997-07       Impact factor: 4.402

7.  Assessment of oxidative, inflammatory, and fibrinolytic biomarkers and DNA strand breakage in hypercholesterolemia.

Authors:  Renata da Silva Pereira; Etiane Tatsch; Guilherme Vargas Bochi; Helena Kober; Thiago Duarte; Greice Franciele Feyh dos Santos Montagner; José Edson Paz da Silva; Marta Maria Medeiros Frescura Duarte; Ivana Beatrice Mânica da Cruz; Rafael Noal Moresco
Journal:  Inflammation       Date:  2013-08       Impact factor: 4.092

8.  Streptozotocin-resistant BRIN-BD11 cells possess wide spectrum of toxin tolerance and enhanced insulin-secretory capacity.

Authors:  Hui-Kang Liu; Jane T McCluskey; Neville H McClenghan; Peter R Flatt
Journal:  Endocrine       Date:  2007-09-29       Impact factor: 3.633

9.  Involvement of ATM/ATR-p38 MAPK cascade in MNNG induced G1-S arrest.

Authors:  Ke-Qing Zhu; Suo-Jiang Zhang
Journal:  World J Gastroenterol       Date:  2003-09       Impact factor: 5.742

10.  Biopure MTAD Induces DNA Damage but Not Cellular Death: An In Vitro Study.

Authors:  Juliana Soares Roter Marins; Luciana Moura Sassone; Daniel Araki Ribeiro
Journal:  Eur J Dent       Date:  2009-10
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