Literature DB >> 7682879

Protein dynamics studied by rotating frame 15N spin relaxation times.

T Szyperski1, P Luginbühl, G Otting, P Güntert, K Wüthrich.   

Abstract

Conformational rate processes in aqueous solutions of uniformly 15N-labeled pancreatic trypsin inhibitor (BPTI) at 36 degrees C were investigated by measuring the rotating frame relaxation times of the backbone 15N spins as a function of the spin-lock power. Two different intramolecular exchange processes were identified. A first local rate process involved the residues Cys38 and Arg39, had a correlation time of about 1.3 ms, and was related to isomerization of the chirality of the disulfide bond Cys14-Cys38. A second, faster motional mode was superimposed on the disulfide bond isomerization and was tentatively attributed to local segmental motions in the polypeptide sequence -Cys14-Ala15-Lys16-. The correlation time for the overall rotational tumbling of the protein was found to be 2 ns, using the assumption that relaxation is dominated by dipolar coupling and chemical shift anisotropy modulated by isotropic molecular reorientation.

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Year:  1993        PMID： 7682879     DOI： 10.1007/bf00178259

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  11 in total

1.  Efficient analysis of protein 2D NMR spectra using the software package EASY.

Authors:  C Eccles; P Güntert; M Billeter; K Wüthrich
Journal:  J Biomol NMR       Date:  1991-07       Impact factor: 2.835

2.  Backbone dynamics of calcium-loaded calbindin D9k studied by two-dimensional proton-detected 15N NMR spectroscopy.

Authors:  J Kördel; N J Skelton; M Akke; A G Palmer; W J Chazin
Journal:  Biochemistry       Date:  1992-05-26       Impact factor: 3.162

3.  Protein structures in solution by nuclear magnetic resonance and distance geometry. The polypeptide fold of the basic pancreatic trypsin inhibitor determined using two different algorithms, DISGEO and DISMAN.

Authors:  G Wagner; W Braun; T F Havel; T Schaumann; N Go; K Wüthrich
Journal:  J Mol Biol       Date:  1987-08-05       Impact factor: 5.469

4.  Carbon-13 nuclear magnetic resonance relaxation studies of internal mobility of the polypeptide chain in basic pancreatic trypsin inhibitor and a selectively reduced analogue.

Authors:  R Richarz; K Nagayama; K Wüthrich
Journal:  Biochemistry       Date:  1980-11-11       Impact factor: 3.162

5.  Analysis of the backbone dynamics of interleukin-1 beta using two-dimensional inverse detected heteronuclear 15N-1H NMR spectroscopy.

Authors:  G M Clore; P C Driscoll; P T Wingfield; A M Gronenborn
Journal:  Biochemistry       Date:  1990-08-14       Impact factor: 3.162

6.  Sequential resonance assignments in protein 1H nuclear magnetic resonance spectra. Basic pancreatic trypsin inhibitor.

Authors:  G Wagner; K Wüthrich
Journal:  J Mol Biol       Date:  1982-03-05       Impact factor: 5.469

7.  Application of phase sensitive two-dimensional correlated spectroscopy (COSY) for measurements of 1H-1H spin-spin coupling constants in proteins.

Authors:  D Marion; K Wüthrich
Journal:  Biochem Biophys Res Commun       Date:  1983-06-29       Impact factor: 3.575

8.  Backbone dynamics of proteins as studied by 15N inverse detected heteronuclear NMR spectroscopy: application to staphylococcal nuclease.

Authors:  L E Kay; D A Torchia; A Bax
Journal:  Biochemistry       Date:  1989-11-14       Impact factor: 3.162

9.  Fast internal main-chain dynamics of human ubiquitin.

Authors:  D M Schneider; M J Dellwo; A J Wand
Journal:  Biochemistry       Date:  1992-04-14       Impact factor: 3.162

10.  Backbone dynamics of the Bacillus subtilis glucose permease IIA domain determined from 15N NMR relaxation measurements.

Authors:  M J Stone; W J Fairbrother; A G Palmer; J Reizer; M H Saier; P E Wright
Journal:  Biochemistry       Date:  1992-05-12       Impact factor: 3.162
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  66 in total

1.  Estimating the time scale of chemical exchange of proteins from measurements of transverse relaxation rates in solution.

Authors:  R Ishima; D A Torchia
Journal:  J Biomol NMR       Date:  1999-08       Impact factor: 2.835

2.  Separating the contributions to 15N transverse relaxation in a fibronectin type III domain.

Authors:  A E Meekhof; S M Freund
Journal:  J Biomol NMR       Date:  1999-05       Impact factor: 2.835

3.  Comparison of the solution conformation and dynamics of antifreeze glycoproteins from Antarctic fish.

Authors:  A N Lane; L M Hays; N Tsvetkova; R E Feeney; L M Crowe; J H Crowe
Journal:  Biophys J       Date:  2000-06       Impact factor: 4.033

4.  Microsecond time scale dynamics in the RXR DNA-binding domain from a combination of spin-echo and off-resonance rotating frame relaxation measurements.

Authors:  F A Mulder; P J van Tilborg; R Kaptein; R Boelens
Journal:  J Biomol NMR       Date:  1999-03       Impact factor: 2.835

5.  Solution structure and backbone dynamics of an omega-conotoxin precursor.

Authors:  D P Goldenberg; R E Koehn; D E Gilbert; G Wagner
Journal:  Protein Sci       Date:  2001-03       Impact factor: 6.725

6.  Protein dynamics in supercooled water: the search for slow motional modes.

Authors:  Jeffrey L Mills; Thomas Szyperski
Journal:  J Biomol NMR       Date:  2002-05       Impact factor: 2.835

7.  Reduced-dimensionality NMR spectroscopy for high-throughput protein resonance assignment.

Authors:  Thomas Szyperski; Deok C Yeh; Dinesh K Sukumaran; Hunter N B Moseley; Gaetano T Montelione
Journal:  Proc Natl Acad Sci U S A       Date:  2002-06-11       Impact factor: 11.205

8.  Off-resonance R1rho relaxation outside of the fast exchange limit: an experimental study of a cavity mutant of T4 lysozyme.

Authors:  Dmitry M Korzhnev; Vladislav Yu Orekhov; Frederick W Dahlquist; Lewis E Kay
Journal:  J Biomol NMR       Date:  2003-05       Impact factor: 2.835

9.  Rapid analysis of protein backbone resonance assignments using cryogenic probes, a distributed Linux-based computing architecture, and an integrated set of spectral analysis tools.

Authors:  Daniel Monleón; Kimberly Colson; Hunter N B Moseley; Clemens Anklin; Robert Oswald; Thomas Szyperski; Gaetano T Montelione
Journal:  J Struct Funct Genomics       Date:  2002

10.  Resonance assignments for the 21 kDa engineered fluorescein-binding lipocalin FluA.

Authors:  Gaohua Liu; Jeffrey L Mills; Tracy A Hess; Seho Kim; Jack J Skalicky; Dinesh K Sukumaran; Eriks Kupce; Arne Skerra; Thomas Szyperski
Journal:  J Biomol NMR       Date:  2003-10       Impact factor: 2.835
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