Literature DB >> 7663145

Determination of heteronuclear three-bond J-coupling constants in peptides by a simple heteronuclear relayed E.COSY experiment.

J M Schmidt1, R R Ernst, S Aimoto, M Kainosho.   

Abstract

A simple heteronuclear relayed E.COSY pulse sequence with a minimum number of pulses is proposed for the quantitative determination of heteronuclear three-bond J-coupling constants in uniformly 13C-enriched polypeptide samples. Numerous heteronuclear three-bond coupling constants, including 3JHNC, 3JHNC beta, 3JH beta C, and 3JH alpha C gamma, can be determined for each residue from a single heteronuclear relayed E.COSY spectrum. Couplings relevant for stereospecific assignments as well as for the determination of dihedral angles in the amino acid backbone and in side chains are obtained. The method is demonstrated on the uniformly 13C-enriched decapeptide antamanide (-Val1-Pro2-Pro3-Ala4-Phe5-Phe6-Pro7-Pro8-Phe9-Phe1 0-).

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Year:  1995        PMID： 7663145     DOI： 10.1007/bf00417495

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  14 in total

1.  Measurement of two-bond JCOH alpha coupling constants in proteins uniformly enriched with 13C.

Authors:  G W Vuister; A Bax
Journal:  J Biomol NMR       Date:  1992-07       Impact factor: 2.835

2.  Determination of H(N),H (α) and H (N),C' coupling constants in (13)C, (15)N-labeled proteins.

Authors:  R Weisemann; H Rüterjans; H Schwalbe; J Schleucher; W Bermel; C Griesinger
Journal:  J Biomol NMR       Date:  1994-03       Impact factor: 2.835

3.  Tertiary templates for proteins. Use of packing criteria in the enumeration of allowed sequences for different structural classes.

Authors:  J W Ponder; F M Richards
Journal:  J Mol Biol       Date:  1987-02-20       Impact factor: 5.469

4.  Conformation of amino acid side-chains in proteins.

Authors:  J Janin; S Wodak
Journal:  J Mol Biol       Date:  1978-11-05       Impact factor: 5.469

5.  A powerful method of sequential proton resonance assignment in proteins using relayed 15N-1H multiple quantum coherence spectroscopy.

Authors:  A M Gronenborn; A Bax; P T Wingfield; G M Clore
Journal:  FEBS Lett       Date:  1989-01-16       Impact factor: 4.124

6.  Conformation of valine side chains in ribonuclease T1 determined by NMR studies of homonuclear and heteronuclear 3J coupling constants.

Authors:  Y Karimi-Nejad; J M Schmidt; H Rüterjans; H Schwalbe; C Greisinger
Journal:  Biochemistry       Date:  1994-05-10       Impact factor: 3.162

7.  Measurement of 15N-13C J couplings in staphylococcal nuclease.

Authors:  F Delaglio; D A Torchia; A Bax
Journal:  J Biomol NMR       Date:  1991-11       Impact factor: 2.835

8.  Multi-conformational peptide dynamics derived from NMR data: a new search algorithm and its application to antamanide.

Authors:  R Brüschweiler; M Blackledge; R R Ernst
Journal:  J Biomol NMR       Date:  1991-05       Impact factor: 2.835

9.  Determination of heteronuclear long-range couplings to heteronuclei in natural abundance by two- and three-dimensional NMR spectroscopy.

Authors:  P Schmieder; M Kurz; H Kessler
Journal:  J Biomol NMR       Date:  1991-11       Impact factor: 2.835

10.  HNCCH-TOCSY, a triple resonance experiment for the correlation of backbone 13C alpha and 15N resonances with aliphatic side-chain proton resonances and for measuring vicinal 13CO,1H beta coupling constants in proteins.

Authors:  R Weisemann; F Löhr; H Rüterjans
Journal:  J Biomol NMR       Date:  1994-07       Impact factor: 2.835
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  4 in total

1.  Self-consistent 3J coupling analysis for the joint calibration of Karplus coefficients and evaluation of torsion angles.

Authors:  J M Schmidt; M Blümel; F Löhr; H Rüterjans
Journal:  J Biomol NMR       Date:  1999-05       Impact factor: 2.835

2.  Alternative E.COSY techniques for the measurement of 3J(C (i) (') (-1),C (i) (beta) ) and (3) J(H (i) (N) ,C (i) (beta) ) coupling constants in proteins.

Authors:  F Löhr; H Rüterjans
Journal:  J Biomol NMR       Date:  1999-03       Impact factor: 2.835

3.  Heteronuclear relayed E.COSY revisited: determination of 3J(H(alpha),C(gamma)) couplings in Asx and aromatic residues in proteins.

Authors:  F Löhr; C Pérez; R Köhler; H Rüterjans; J M Schmidt
Journal:  J Biomol NMR       Date:  2000-09       Impact factor: 2.835

4.  Heteronuclear relayed E.COSY applied to the determination of accurate 3J(HN,C') and 3J(H beta,C') coupling constants in desulfovibrio vulgaris flavodoxin.

Authors:  J M Schmidt; F Löhr; H Rüterjans
Journal:  J Biomol NMR       Date:  1996-03       Impact factor: 2.835
  4 in total

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