Literature DB >> 8787418

Pyrimidine biosynthesis genes (pyrE and pyrF) of an extreme thermophile, Thermus thermophilus.

A Yamagishi1, T Tanimoto, T Suzuki, T Oshima.   

Abstract

We have isolated uracil auxotrophic mutants of an extreme thermophile, Thermus thermophilus. A part of the pyrimidine biosynthetic operon including genes for orotate phosphoribosyltransferase (pyrE) and for orotidine-5'-monophosphate decarboxylase (pyrF) was cloned and sequenced. The pyrE gene can be a bidirectional marker for the gene manipulation system of the thermophile.

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Year:  1996        PMID： 8787418      PMCID： PMC167999          DOI： 10.1128/aem.62.6.2191-2194.1996

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  26 in total

1.  Sequence of the cloned pyr4 gene of Trichoderma reesei and its use as a homologous selectable marker for transformation.

Authors:  J L Smith; F T Bayliss; M Ward
Journal:  Curr Genet       Date:  1991-01       Impact factor: 3.886

2.  Recombination-Deficient Mutants of an Extreme Thermophile, Thermus thermophilus.

Authors:  T Takada; S Akanuma; T Kotsuka; M Tamakoshi; A Yamagishi; T Oshima
Journal:  Appl Environ Microbiol       Date:  1993-08       Impact factor: 4.792

3.  Positive screening and transformation of ura5 mutants in the fungus Podospora anserina: characterization of the transformants.

Authors:  V Razanamparany; J Bégueret
Journal:  Curr Genet       Date:  1986       Impact factor: 3.886

4.  Positive selection for Dictyostelium discoideum mutants lacking UMP synthase activity based on resistance to 5-fluoroorotic acid.

Authors:  D Kalpaxis; I Zündorf; H Werner; N Reindl; E Boy-Marcotte; M Jacquet; T Dingermann
Journal:  Mol Gen Genet       Date:  1991-03

5.  Isolation of a thermostable enzyme variant by cloning and selection in a thermophile.

Authors:  H Liao; T McKenzie; R Hageman
Journal:  Proc Natl Acad Sci U S A       Date:  1986-02       Impact factor: 11.205

6.  Isolation and molecular analysis of the orotidine-5'-phosphate decarboxylase gene (pyrG) of Phycomyces blakesleeanus.

Authors:  J M Díaz-Mínguez; E A Iturriaga; E P Benito; L M Corrochano; A P Eslava
Journal:  Mol Gen Genet       Date:  1990-11

7.  The PYR1 gene of the plant pathogenic fungus Colletotrichum graminicola: selection by intraspecific complementation and sequence analysis.

Authors:  J B Rasmussen; D G Panaccione; G C Fang; R M Hanau
Journal:  Mol Gen Genet       Date:  1992-10

8.  Genetic transformation of the extreme thermophile Thermus thermophilus and of other Thermus spp.

Authors:  Y Koyama; T Hoshino; N Tomizuka; K Furukawa
Journal:  J Bacteriol       Date:  1986-04       Impact factor: 3.490

9.  Development of plasmid cloning vectors for Thermus thermophilus HB8: expression of a heterologous, plasmid-borne kanamycin nucleotidyltransferase gene.

Authors:  M W Mather; J A Fee
Journal:  Appl Environ Microbiol       Date:  1992-01       Impact factor: 4.792

10.  Cloning of the structural gene for orotidine 5'-phosphate carboxylase of Neurospora crassa by expression in Escherichia coli.

Authors:  F P Buxton; A Radford
Journal:  Mol Gen Genet       Date:  1983
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  11 in total

1.  Thermostable repair enzyme for oxidative DNA damage from extremely thermophilic bacterium, Thermus thermophilus HB8.

Authors:  T Mikawa; R Kato; M Sugahara; S Kuramitsu
Journal:  Nucleic Acids Res       Date:  1998-02-15       Impact factor: 16.971

2.  Overexpression, purification and characterization of RecJ protein from Thermus thermophilus HB8 and its core domain.

Authors:  A Yamagata; R Masui; Y Kakuta; S Kuramitsu; K Fukuyama
Journal:  Nucleic Acids Res       Date:  2001-11-15       Impact factor: 16.971

3.  Structure and expression of a pyrimidine gene cluster from the extreme thermophile Thermus strain ZO5.

Authors:  M Van de Casteele; P Chen; M Roovers; C Legrain; N Glansdorff
Journal:  J Bacteriol       Date:  1997-06       Impact factor: 3.490

4.  A new Thermus-Escherichia coli shuttle integration vector system.

Authors:  M Tamakoshi; M Uchida; K Tanabe; S Fukuyama; A Yamagishi; T Oshima
Journal:  J Bacteriol       Date:  1997-08       Impact factor: 3.490

5.  Development of pyrF-based genetic system for targeted gene deletion in Clostridium thermocellum and creation of a pta mutant.

Authors:  Shital A Tripathi; Daniel G Olson; D Aaron Argyros; Bethany B Miller; Trisha F Barrett; Daniel M Murphy; Jesse D McCool; Anne K Warner; Vineet B Rajgarhia; Lee R Lynd; David A Hogsett; Nicky C Caiazza
Journal:  Appl Environ Microbiol       Date:  2010-08-06       Impact factor: 4.792

6.  Characterization of three putative Lon proteases of Thermus thermophilus HB27 and use of their defective mutants as hosts for production of heterologous proteins.

Authors:  Tomoko Maehara; Takayuki Hoshino; Akira Nakamura
Journal:  Extremophiles       Date:  2007-12-22       Impact factor: 2.395

7.  Orotate phosphoribosyltransferase from Corynebacterium ammoniagenes lacking a conserved lysine.

Authors:  Xing Wang; Cuiqing Ma; Xiuwen Wang; Ping Xu
Journal:  J Bacteriol       Date:  2007-10-05       Impact factor: 3.490

8.  The rate and character of spontaneous mutation in Thermus thermophilus.

Authors:  Reena R Mackwan; Geraldine T Carver; Grace E Kissling; John W Drake; Dennis W Grogan
Journal:  Genetics       Date:  2008-08-24       Impact factor: 4.562

9.  Establishment of uracil auxotrophic dikaryotic strains of Lentinula edodes by crossbreeding.

Authors:  Chenli Zhou; Liping Xi; Wenjun Mao; Jianing Wan; Yan Li; Ying Wang; Dapeng Bao
Journal:  Breed Sci       Date:  2017-03-17       Impact factor: 2.086

10.  Occurrence of randomly recombined functional 16S rRNA genes in Thermus thermophilus suggests genetic interoperability and promiscuity of bacterial 16S rRNAs.

Authors:  Kentaro Miyazaki; Natsuki Tomariguchi
Journal:  Sci Rep       Date:  2019-08-02       Impact factor: 4.379
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